- Research article
- Open Access
Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells
© Villena et al.; licensee BioMed Central Ltd. 2014
- Received: 6 May 2013
- Accepted: 7 May 2014
- Published: 16 May 2014
Previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized.
We aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer’s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. L. rhamnosus CRL1505 was able to induce IFN–α and –β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs.
It was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.
- Lactobacillus rhamnosus
- Antiviral immunity
- PIE cells
- Intestinal antigen presenting cells
Around 5.2 million children under five years old die yearly due to preventable infectious diseases like pneumonia and diarrhoea [1, 2]. Among these infectious diseases, viral gastrointestinal infections belong to the most frequent diseases suffered in childhood, especially in the developing world. Rotavirus, a RNA virus, is the most common cause of severe dehydrating diarrhoea in children worldwide [3, 4]. Although there is already a successful rotavirus vaccine in the market, the epidemic in the developing world is far from being controlled [4, 5]. Apart from being not affordable for low-income population groups, it has also been shown that protection induced by natural infection and vaccination is reduced in developing areas, where among other factors, children are infected at an early age and high viral challenge loads are usual . Moreover, Latin America in general and northern Argentina in particular, presents a significant population of malnourished children with its associated burden of otherwise preventable infectious diseases such as rotavirus infections .
Several studies have demonstrated that certain lactic acid bacteria (LAB) strains can exert their beneficial effect on the host through their immunomodulatory activity. In this sense, some studies have centred on whether immunoregulatory probiotic LAB (immunobiotics) might sufficiently stimulate the intestinal immune system to provide protection against viral infections. It was reported that probiotics can exerts some beneficial effects in rotavirus intestinal infections such as shortening the duration of diarrhoea, reducing the number of episodes, lessening rotavirus shedding, normalizing gut permeability and increasing the production of rotavirus-specific antibodies [7–9]. In an attempt to find low-cost alternatives for the prevention of infectious diseases we have developed a new probiotic yogurt, containing the immunobiotic strain Lactobacillus rhamnosus CRL1505, able to improve resistance against respiratory and intestinal infections. Our studies in animal models showed that the administration of L. rhamnosus CRL1505 significantly augmented the resistance of immunocompetent and immunocompromised malnourished mice to intestinal and respiratory pathogens such as Salmonella Typhimurium and Streptococcus pneumoniae[10, 11]. In addition, we performed a randomized controlled trial in order to evaluate the effect of the probiotic yogurt containing L. rhamnosus CRL1505 on both gut and non-gut related illnesses among children . We demonstrated that the CRL1505 strain improved mucosal immunity and reduced the incidence and severity of intestinal and respiratory infections. We registered that 34% of the children who consumed the probiotic yogurt showed some type of infectious event, while in the placebo group this value was higher reaching a 66% of them. Although we did not evaluate aetiology of intestinal and respiratory infections in the clinical study, previous evaluations have shown that viruses, such as rotavirus and respiratory syncytial virus, are the major pathogens, which cause infectious diseases in children in northern Argentina [13, 14]. Therefore, our findings suggested that administration of L. rhamnosus CRL1505 may provide a potential intervention to prevent the course of common childhood viral infections. Some of the mechanisms by which L. rhamnosus CRL1505 exerts its immunomodulatory and antiviral properties have been elucidated [10, 11, 15]. We have recently showed the capacity of the CRL1505 strain to improve the production of antiviral cytokines in the gut and the respiratory tract [10, 11, 15, 16]. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this immunobiotic strain have not been fully characterized.
Intestinal epithelial cells (IECs) are the first cells which encounter exogenous and endogenous as well as pathogenic and non-pathogenic microorganisms . In addition, the gut of vertebrates is rich in antigen-presenting cells (APCs), such as macrophages and dendritic cells (DCs), which are able to recognize foreign antigens or invading pathogens. The epithelium and APCs at the intestinal surfaces express a diverse range of Pattern Recognition Receptors (PRRs) capable of detecting viruses. Epithelial- and APCs-expressed PRRs include cell surface expressed C-type lectins (cell surface variants of the secreted collectins), intra- and extracellular toll-like receptors (TLR), the intracellular RNA-dependent protein kinase (PKR), retinoic acid–inducible gene I (RIG-I) like receptors (RLR) and nucleotide binding domain and leucine-rich repeat containing receptors (NLR) [18–20]. Upon recognition of double-stranded RNA (dsRNA) or its synthetic analogue poly(I:C), TLR3 and RIG-I trigger the activation of the transcription factors IRF-3, NF-kB, and AP-1, which in turn induce type I IFNs (especially IFN-β) and cytokine/chemokine synthesis.
There is a growing interest in studying the swine immune system because of its similarities to the human immune system. We have precisely characterized the functionality of porcine APCs from Peyer’s Patches (PPs) before and also demonstrated that swine PPs-derived adherent cells are a useful in vitro tool for investigating innate responses to pathogenic and probiotic microorganisms . In addition, we have also reported an abundant intracellular expression of TLR3 in a porcine intestinal epithelial (PIE) cell line , which is in line with findings of Liu et al.  that demonstrated that the non-transformed porcine jejunum epithelial cell line (IPEC-J2) expresses TLR3 constitutively. We characterized the immune response triggered by poly(I:C) challenge in PIE cells and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools for studying in vitro the immune response triggered by TLR3/RIG-I on IECs and the interaction between IECs and immune cells [22, 23]. In this study, we therefore aimed to use these porcine in vitro systems to gain insight into the mechanisms involved in the immunomodulatory effect of CRL1505 strain, and concentrated our attention in the crosstalk between L. rhamnosus CRL1505, PIE cells and APCs in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes.
Lactobacillus rhamnosus CRL1505 (Lr1505) and L. rhamnosus CRL1506 (Lr1506) belong to CERELA Culture Collection and were originally isolated from goat milk . These strains were grown in Man-Rogosa-Sharpe (MRS) broth at 37°C. For immunomodulatory assays, overnight cultures were harvested by centrifugation, washed three times with sterile PBS, counted in a Petroff-Hausser counting chamber, and re-suspended in DMEM until use.
PIE cell monocultures
A non-transformed porcine intestinal epithelial cell line (PIE), characterized by its ability to build a monolayer with a cobblestone and epithelial-like morphology and close contacts between cells was used as described before [22, 23]. Briefly, PIE cells were grown on type I collagen-coated dishes using DMEM (Gibco, Japan) supplemented with 10% fetal calb serum (FCS, Sigma). PIE cells were incubated at 37°C and 5% CO2. Passages were done by treating the monolayer with sucrose/EDTA for 4 min and detaching the cells with 0.04% trypsin.
Isolation of adherent population from swine Peyer’s patches (PPs)
Suspensions of porcine PP immunocompetent cells were prepared from adult swine intestine. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Guidelines for Animal Experimentation of Tohoku University, Sendai, Japan. The present study was approved by the Institution Animal Care and Use Committee of Tohoku University with a permitted No. 2011-noudou-5 and all efforts were made to minimize suffering. Swine PPs were cut into small pieces and gently pressed through a nylon mesh to prepare single immune cell suspensions. After several washes in complete RPMI medium, residual erythrocytes were lysed in 0.2% NaCl followed by a hypertonic rescue in 1.5% NaCl. Finally, immune cells were fractioned by density gradient centrifugation using Lympholyte Mammal (Cedarlane, Corby, Canada) and the mononuclear cell suspension containing a mixed population of T, B and antigen presenting cells (APCs) was suspended in complete DMEM supplemented with 10% FCS, 50 μg/ml penicillin/streptomycin and 50 μg/ml gentamycin (Nacalai Tesque, Kyoto, Japan) [22, 23]. APCs (macrophages and DCs) were separated by their ability to adhere to glass as described before . Briefly, cell suspensions (5 × 107 cells/well) were placed onto 2-well glass plates (Iwaki, Tokyo, Japan) and incubated for 2 h at 37°C and 5% CO2 to allow cells to adhere to the glass surface. Subsequently, they were washed gently with complete RPMI 1640 medium (Sigma) to remove non-adherent cells. With this methodology a mix population containing CD172a+CD11R1−, CD172a−CD11R1low and CD172a+CD11R1high cells was obtained .
Immunomodulatory effect of lactobacilli
Evaluation of the immunomodulatory activity of L. rhamnosus CRL1505 and L. rhamnosus CRL1506 was performed using PIE cells and PPs-derived adherent cells [21–23]. For immunomodulatory assays, 1.5 × 104 PIE cells/well were plated onto type I collagen coated 24-well plates (Iwaki, Tokyo, Japan). Three days later, cell monolayers were washed, added with lactobacilli (5 × 108 cells/well) and incubated for 48 h at 37°C and 5% CO2, after which cells were vigorously washed and harvested for total RNA isolation for cytokine expression profiles. In a second experiment to study immunomodulation of antiviral innate responses with lactobacilli, PIE cell monolayers were incubated 48 h with lactobacilli, washed three times to eliminate possible stimulants and were further stimulated with poly(I:C) to mimic viral infection at the indicated times. Again, RNA was isolated for studying expression profiles [22, 23]. Adherent cells were plated at a density of 1.5 × 106 cells/well in 12-well type I collagen-coated plates (Iwaki) or in 2-well glass plates (Iwaki). Lactobacilli were added to each well (5 × 108 cells/ml) and incubated for further 16 h. For evaluation of the modulation of antiviral responses by lactobacilli in APCs, adherent cells were prepared as indicated before and 16 h later, each well was washed vigorously with medium at least 3 times to eliminate bacteria; and finally the porcine cells were stimulated with poly(I:C) for the time indicated . In addition, unlabelled anti-TLR2 rabbit IgG or anti-TLR9 rabbit IgG (Santa Cruz, Santa Cruz, CA) were used in blocking experiments. Cultured cells were incubated with the unlabelled anti-TLR2 or anti-TLR9 antibodies for 12 h before stimulation with lactobacilli.
Lactobacilli immunomodulatory activity in PIE-adherent cells co-culture system
Porcine PPs adherent cells suspensions were prepared as described above. In the Transwell culture system, PIE cells were seeded in the apical surface at a concentration of 1.5 × 105 cells/well in 12-well tissue culture plates (Transwell-Col. (PTFE), pore size 0.2 mm) while porcine PPs adherent cells were seeded in the basolateral compartment at a concentration of 2 × 107 cells/well [22, 23]. For the evaluation of the immunomodulatory activity of lactobacilli in the PIE-immune cell co-culture system, the apical surface containing PIE cells was stimulated with lactobacilli strains for 48 h and then washed twice with PBS. Finally, PIE cells were stimulated with poly(I:C) for 12 h.
qRT-PCR of mRNA expression in PIE and immune cells
Total RNA from each stimulated monolayer (PIE cell monoculture or co-culture) was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. cDNA was synthesized using a Quantitect Reverse Transcription kit (Qiagen, Tokyo, Japan). qRT-PCR was carried out in a 7300 Real-time PCR System (Applied Biosystems, Warrington, Cheshire, UK) using Platinum SYBR Green qPCR SuperMix UDG with ROX (Invitrogen). The primers for IFN-α, IFN-β, TNF-α, IFN-γ, IL-1β, TGF-β, IL-2, IL-6, IL-10 and IL-12p40 used in this study were described previously . The PCR cycling conditions were 5 min at 50°C; followed by 2 min at 95°C; then 40 cycles of 15 sec at 95°C, 30 sec at 60°C and 30 sec at 72°C. The reaction mixture contained 5 μl cDNA and 15 μl master mix including sense and antisense primers. Expression of the house-keeping gene b-actin was assessed in each sample, as an internal control to normalize differences between samples and to calculate the relative index.
Flow cytometric analysis
Flow cytometry was used to assess expression of MHC-II, CD80/86, IFN-γ, IL-1β, IL-6 and IL-10 in PPs CD172a+CD11R1−, CD172a−CD11R1low and CD172a+CD11R1high cells. Adherent cells were isolated as described above and labeled with primary antibodies: anti-porcine CD172a-PE SWC3 IgG1 (Southern Biotech), anti-porcine CD11R1-IgG1 (AbD Serotec), anti-porcine MHC-II-IgG2a (VMRD), anti-porcine gamma interferon (IFN-γ)-IgG2b (R&D Systems, Minneapolis, MN), anti-porcine interleukin-10 (IL-10)-IgG2b (R&D Systems), anti-porcine IL-1β/IL-1 F2-IgG1 (R&D Systems), and anti-porcine IL-6-IgG2b (R&D Systems). The binding of unlabeled monoclonal antibodies was visualized using the following secondary antibodies: anti-mouse IgG1-peridinin chlorophyll protein (PerCP)/Cy5.5 (Bio Legend, San Diego, CA), anti-mouse IgG2a-FITC (AbD Serotec), anti-rabbit IgG-Alexa Fluor 489 (Santa Cruz), anti-mouse IgG2b-FITC (AbD Serotec), and anti-mouse IgG-FITC (AbD Serotec) . In addition, expression levels of CD80/86 proteins were evaluated using a human CD152 (cytotoxic-T- lymphocyte-associated antigen 4) Ig/FITC fusion protein (Ancell, Bay- port, MN). Cells stained with irrelevant mouse IgG-FITC, IgG2b-FITC, IgG2a-PerCP, IgG2b-PE, IgG2a-PE, or IgG1-PE antibodies (eBioscience, San Diego, CA) were included as isotype controls. Analysis of the stained cells was performed using a FACSCalibur flow cytometer (BD, Franklin Lakes, NJ), which was equipped with Cell-Quest software. Data analysis was performed using FlowJo software (Tree Star, Ashland, OR) .
Statistical analyses were performed using the GLM and REG procedures available in the SAS computer program (SAS, 1994). Comparisons between mean values were carried out using one-way analysis of variance and Fisher’s least-significant-difference (LSD) test. P < 0.05 were considered significant.
Lactobacillus rhamnosusstrains differentially modulate cytokines transcriptional profiles of PIE cells and PPs derived adherent cells
In a similar setting but using APCs, Lr1505 and Lr1506 also showed a differential effect on the mRNA expression of some cytokines as shown in Figure 1B. Although both strains stimulated adherent cells, Lr1505 showed a stronger enhancing influence than Lr1506 on the expression of mRNA coding for IL-1β, IFN-γ, IL-2, IL-12 and IL-10 (Figure 1B). Both lactobacilli slightly but significantly increased the mRNA synthesis of IL-6 and TNF-α to similar levels. In contrast to the results seen in PIE cells, there was no meaningful effect on the mRNA expression of type I IFN (Figure 1B). Furthermore, TGF-β mRNA levels were not affected by the stimulation with lactobacilli.
L. rhamnosusCRL1505 and CRL1506 stimulate PPs APCs and distinctly modulate cytokine production
We next studied whether Lr1505 and Lr1506 were able to affect the expression of two cellular surface markers for APCs activation: MHC-II and CD80/CD86. Adherent cells isolated from swine Peyer’s Patches can be grouped as CD172a+CD11R1high, CD172a−CD11R1low and CD172a+CD11R1− cells . Although more detailed functional studies are needed to accurately define each population, it has been suggested that CD172a+CD11R1high and CD172a−CD11R1low cells could be considered as DCs and CD172a+CD11R1− cells could be considered as macrophages . In these three cell populations, both strains exerted an up-regulation of the antigen presenting and co-stimulatory molecules MHC-II and CD80/86, when compared to the non-stimulated control (Figure 1C) indicating that these immunobiotic microorganisms were able to activate APCs. In all cases the MIF values in Lr1505-treated cells almost doubled the MIF presented by control cells (Figure 1C). APCs were similarly modulated by Lr1506 (data not shown). We also analysed by flow cytometry the levels of IL-1β, IL-6, IFN-γ, and IL-10 on the three populations of adherent cells: CD172a+CD11R1−, CD172a−CD11R1low and CD172a+CD11R1high (Figure 1D). In CD172a+CD11R1− cells both strains Lr1505 and Lr1506 slightly but significantly enhanced the post-translational expression levels of IL-1β, IL-6, and IL-10, while the IFN-γ levels remained unchanged (Figure 1D). In CD172a−CD11R1low cells, both strains had a similar effect on the expression of IL-1β, IL-6 and IFN-γ, whereas IL-10 levels were not modified. In contrast, in CD172a−CD11R1high cells IL-10 protein levels were up-regulated by both strains, being Lr1505 the strain which showed the strongest stimulation (Figure 1D). In addition, IL-1β was modulated only by Lr1505 but neither IL-6 nor IFN-γ levels were affected by the stimulation of CD172a−CD11R1high cells with lactobacilli (Figure 1D). These results correlated with the mRNA expression profiles shown before (Figure 1B).
Lactobacilli influence mRNA expression of type I IFN and inflammatory cytokines in PIE cells after poly(I:C) challenge
Lactobacilli activate APCs and differentially modulate the expression of cytokines and activation markers in response to poly(I:C)
In parallel experiments using the same stimulation protocols, we studied the expression of surface activation markers and protein cytokine levels by flow cytometry in CD172a+CD11R1−, CD172a−CD11R1low and CD172a+CD11R1high adherent cells (Figure 3B). Challenge with poly(I:C) significantly increased the expression of surface molecules MHC-II and CD80/86 in the three populations of APCs. In addition, we observed that lactobacilli-treated cells showed higher levels of MHC-II and CD80/86 when compared to control cells with the exception of CD80/86 in Lr1506-treated CD172a+CD11R1high cells that was similar to controls (Figure 3B). We also observed differences in the up-regulation of both molecules when comparing Lr1505 and Lr1506, since MCH-II levels in CD172a−CD11R1low and CD172a+CD11R1high adherent cells and CD80/86 levels in the three populations of APCs were higher in Lr1505-treated cells than in those stimulated with Lr1506 (Figure 3B). We also observed an up-regulation of IL-1β, IL-6, IL-10 and IFN-γ in poly(I:C) challenged APCs (Figure 3B) after being treated with L. rhamnosus strains. When studying the influence of lactobacilli on the distinct populations of APCs, we observed a differential behaviour towards each cell group. IL-1β, IL-6 and IFN-γ levels were significantly higher in lactobacilli-treated CD172a−CD11R1low cells when compared to controls. Moreover, Lr1505 was more efficient than Lr1506 to up-regulate the levels of the three cytokines in that cell population (Figure 3B). On the other hand, IL-10 levels were significantly higher in lactobacilli-treated CD172a+CD11R1− and CD172a+CD11R1high cells when compared to controls. Moreover, Lr1505 was more efficient than Lr1506 to up-regulate the levels of IL-10 in both cell populations (Figure 3B).
Lactobacilli differentially modulate cytokine expression in response to poly(I:C) challenge in APCs co-cultured with PIE cells
TLR2 but not TLR9 would be involved in the immunoregulatory effect of lactobacilli
Rotavirus represents one of the prevailing causes of infectious gastroenteritis in humans worldwide [3, 4, 6]. An initial and essential step in the viral infection cycle of rotavirus is entering and replicating in IECs of the small intestine . IECs have been well defined as sentinels, because they are the first cells which encounter microorganisms and are not only a physical barrier but they recognize different types of PAMPs via PRRs, which are selectively expressed on the cell surface, internal compartments or cytoplasm. Upon virus internalization, dsRNA molecules are generated in infected cells . These molecules are typical of many viral infections including rotavirus. Viral dsRNA activate PRRs such as TLR3, RIG-I, and MDA-5, which signal host cellular responses in order to try to control viral infection [25–27]. IFNs and IFN-regulated gene products are then synthesized and play a key role in the host response for clearing viruses. Type I together with type II IFNs are able to limit rotavirus infection in vitro and their levels are augmented in rotavirus-infected children and animals [18, 28, 29]. Recently, it has been proposed that IFNs signalling is not only beneficial to the host, but it may also enhance rotavirus replication at the first stages of infection . Nevertheless, other in vivo studies have shown a markedly increase in the virulence of certain strains of rotavirus when IFNs signalling was blocked during infection . Furthermore, the fact that rotavirus has evolved mechanisms to manipulate IFNs signalling such as the type I IFNs damping NSP1 protein , strongly suggests that IFNs are crucial to limit infection. Therefore, approaches aiming to modulate pathways leading to IFNs production may provide valuable tools to increase natural viral defence mechanisms. Herein we show evidence of how IECs can be modulated by immunobiotic L. rhamnosus in a strain-dependent fashion to enhance antiviral responses. For instance, Lr1506 was a stronger inducer of both IFN-α and IFN-β than Lr1505. In addition, these strains primed PIE cells to respond to the dsRNA analogue poly(I:C), as the cells responded with a significantly stronger synthesis of mRNA encoding for type I IFNs than non-treated cells. Moreover, the exposition of IECs to Lr1506 resulted in a significantly stronger up-modulation of type I IFNs mRNA expression than the treatment with Lr1505.
Although activation of PPRs signalling pathways, especially upon stimulation with their respective ligands have been extensively studied, research on the specific effect and modulation capability of probiotics including whole live LAB is more recent and in general includes different species of Gram-positive bacteria. We have reported previously, the modulation of type I IFNs in PIE cells by lactobacillus strains, specifically Lactobacillus casei MEP221106 . Other studies on type I IFN induction and/or modulation by lactobacilli have only been reported for professional immune cells such as macrophages, DCs and PBMC but are rare for IECs. Furthermore, our results using blocking anti-TLR2 and anti-TLR9 antibodies ruled out the involvement of both TLR2 and TLR9 (the classical TLRs associated to LAB recognition) in the primary induction of type I IFNs or the enhancement of IFN-α and -β synthesis upon poly(I:C) challenge induced by Lr1505 and Lr1506 in PIE cells. Further studies are needed in order to find the PRRs involved in the recognition of lactobacilli leading to IFN-α and IFN-β expression in PIE cells.
IECs are able to initiate and in a minor extent to regulate the immune response to bacteria and viruses  being able to secrete several pro-inflammatory cytokines such as MCP-1, IL-6 and TNF-α on stimulation by pathogens. Both Lr1505 and Lr1506 were able to induce IL-6 and TNF-α mRNA expression in PIE cells but not MCP-1. This fact contrasts with a previous report about another probiotic strain, L. casei CRL431, which induces MCP-1 in murine IECs, which may be explained as both a strain-specific and/or a host-specific phenomenon . In addition, not all IEC lines (e.g.: Caco-2, HT29, T84) are able to produce the same cytokine profile upon stimulation, and therefore, there are contradictory reports on the ability of lactobacilli and other Gram-positive commensal bacteria to induce IL-6 in IECs. Thus, as already suggested, this may be one advantage of working with IECs primary cultures . Vinderola et al.  reported induction of IL-6 by probiotic lactobacilli in normal murine IECs as it was also the case for the effect on porcine IECs reported in this study. Our results using anti-TLR2 blocking antibodies proved that TLR2 is responsible for the recognition of lactobacilli and induction of IL-6 and TNF-α, which agrees with the results of Castillo et al. .
Dendritic cells are leading gatekeepers and regulators of immunity, which are present in all tissues, especially at the interface with the external environment, such as the mucosa of the gastrointestinal tract . In the gut, they play a fundamental role as they orchestrate the subtle equilibrium between tolerance and protection against infection . We and others have reported that probiotic lactobacilli are able to differentially stimulate and modulate DCs in vitro[22, 23, 37–40]. Thus, we wanted to study how the two immunobiotic L. rhamnosus strains reported here functionally modulate porcine PPs-derived adherent immune cells (CD172a+CD11R1−, CD172a−CD11R1low and CD172a+CD11R1high cells). The main effect of incubating L. rhamnosus with the single populations of immune adherent cells, resulted in differential mRNA expression of the key polarizing cytokines IL-1β, IL-6 and IFN-γ, which determine the fate of naïve T-cells. Lr1505 was the strain with the highest capacity to functionally modulate APCs. Considering CD172a+CD11R1high and CD172a−CD11R1low cells as DCs , and as such with the ability to favour Th1, Th2, Th17 or Treg immune responses, the increases in both IFN-γ and IL-12 induced especially by Lr1505, may lead to a Th1 response if we extrapolate this data to an in vivo situation. Furthermore, IFN-γ and IL-1β have been shown to have a direct effect on IECs inducing an antiviral program, which inhibits rotavirus entry [41, 42].
On the other hand, Lr1505 also induced IL-10 mRNA and protein expression, which is an immunoregulatory cytokine that avoids inflammatory-tissue injury during infections. Zhou et al.  provided direct evidence that aberrant activation of intestinal immunity induced by poly(I:C) or purified rotavirus genomic dsRNA causes a breakdown of the mucosal homeostasis, leading to mucosal damage. Moreover, it was reported that the induction of the regulatory IL-10 plays an important role to control the inflammatory process upon a viral infection to minimize tissue injury [39, 44]. Then, the improved production of IL-10 induced by Lr1505 in APCs after the challenge with poly(I:C) could have an important protective effect during intestinal viral infections.
It is well known that commensal microbiota interacts with cells of the intestinal mucosa via TLR  but not all bacteria have the ability to modulate immune responses, as this is a strain specific characteristic. As lactobacilli may be recognized by APCs through the peptidoglycan and lipoteichoic acid in their cell walls and/or CpG motifs in their DNA, we used anti-TLR2 and anti-TLR9 antibodies to block recognition via the respective receptors in order to elucidate whether they were responsible for the observed immunoregulatory activity of lactobacilli in APCs. TLR2 is one of the PRRs that would be of great importance for the immunomodulatory effect of probiotic microorganisms in APCs. Immunoenhancing lactobacilli are able to increase the expression of TLR2 in DCs and macrophages isolated from PPs in mice  and in human myeloid DCs . Moreover, Weiss et al.  reported a TLR2-dependent mechanism for L. acidophilus NCFM, whose IFN-β expression was markedly reduced in TLR-2−/− DCs. In our experiments, the main effect observed on type I IFNs was observed in PIE cells and not in immune cells. After the challenge of APCs with poly(I:C), we observed a weak enhancement of type I IFNs mRNA expression, which was only 3 h after stimulation and therefore was not further studied. On the contrary, we observed a clear involvement of TLR2 signalling pathway in the up-modulation of IL-1β, IL-6, IL-10 and IFN-γ in APCs exerted by both L. rhamnosus strains alone and following a poly(I:C) challenge. In addition, the lactobacilli reported by Plantinga et al.  induced cytokines in DCs in a TLR9-dependent manner, contrasting our results which show no relationship between TLR9 and the immunoregulatory effect of Lr1505 or Lr1506.
Both Lr1505 and Lr1506 were able to induce IFN–α and –β mRNA expression in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of TLR2 or TLR9 signalling. However, Lr1506 showed a higher capacity to improve levels of IFN-α and IFN-β in IECs when compared with Lr1505, which is in line with our previously reported in vivo results, showing higher levels of IFN-α and IFN-β in intestinal fluids of Lr1506-treated than in Lr1505-treated mice . Considering that type I IFNs up-regulate several genes involved in viral defence and genes of major importance for the development of a strong cellular response, we hypothesize that Lr1506 may play an important role in the improvement of innate immune responses against intestinal virus, especially in IECs.
In addition, both lactobacilli induced expression of IL-6 and TNF-α via TLR2 in IECs, being Lr1505 the stronger modulator of these cytokines. Furthermore, although both strains were able to significantly increase surface molecules expression and cytokine production in intestinal APCs, Lr1505 had a stronger effect both when applied alone or combined with a posterior poly(I:C) challenge. The improved Th1 response induced by Lr1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs (Figure 7). Considering that TLR signalling is a crucial aspect of innate defence [48, 49], but if uncontrolled at mucosal surfaces, it would be pathological, it is important to highlight again the fact that IL-10 was also significantly up-regulated by Lr1505, suggesting that the inflammatory conditions may be held under control (Figure 7). These in vitro results are in line with our previous findings showing that Lr1505 was more efficient than Lr1506 for increasing the levels of IFN-γ, IL-10 and IL-6 in the intestine of mice .
It was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies . The use of L. rhamnosus CRL1505 and L. rhamnosus CRL1506 as modulators of innate immunity and inductors of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge. To evaluate in vitro and in vivo the capacity of both strains to protect against rotavirus infection is an interesting topic for future research.
This study was partially supported by a Grant-in-Aid for Scientific Research (KAKENHI) (B) (No. 24380146) from the Japan Society for the Promotion of Science (JSPS) to Dr. H. Kitazawa. We thank Leonardo Albarracin for his help with the design and development of figures.
- Bryce J, Black RE, Walker N, Bhutta ZA, Lawn JE, Steketee RW: Can the world afford to save the lives of 6 million children each year?. Lancet. 2005, 365 (9478): 2193-2200. 10.1016/S0140-6736(05)66777-3.View ArticlePubMedGoogle Scholar
- Lopez AD, Mathers CD, Ezzati M, Jamison DT, Murray CJ: Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data. Lancet. 2006, 367 (9524): 1747-1757. 10.1016/S0140-6736(06)68770-9.View ArticlePubMedGoogle Scholar
- Parashar UD, Gibson CJ, Bresee JS, Glass RI: Rotavirus and severe childhood diarrhea. Emerg Infect Dis. 2006, 12 (2): 304-306. 10.3201/eid1202.050006.PubMed CentralView ArticlePubMedGoogle Scholar
- Greenberg HB, Estes MK: Rotaviruses: from pathogenesis to vaccination. Gastroenterology. 2009, 136 (6): 1939-1951. 10.1053/j.gastro.2009.02.076.PubMed CentralView ArticlePubMedGoogle Scholar
- Tate JE, Patel MM, Cortese MM, Lopman BA, Gentsch JR, Fleming J, Steele AD, Parashar UD: Remaining issues and challenges for rotavirus vaccine in preventing global childhood diarrheal morbidity and mortality. Expert Rev Vaccines. 2012, 11 (2): 211-220. 10.1586/erv.11.184.View ArticlePubMedGoogle Scholar
- Angel J, Franco MA, Greenberg HB: Rotavirus immune responses and correlates of protection. Curr Opin Virol. 2012, 2 (4): 419-425. 10.1016/j.coviro.2012.05.003.PubMed CentralView ArticlePubMedGoogle Scholar
- Basu S, Paul DK, Ganguly S, Chatterjee M, Chandra PK: Efficacy of high-dose Lactobacillus rhamnosus GG in controlling acute watery diarrhea in Indian children: a randomized controlled trial. J Clin Gastroenterol. 2009, 43 (3): 208-213. 10.1097/MCG.0b013e31815a5780.View ArticlePubMedGoogle Scholar
- Liu F, Li G, Wen K, Bui T, Cao D, Zhang Y, Yuan L: Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics. Viral Immunol. 2010, 23 (2): 135-149. 10.1089/vim.2009.0088.PubMed CentralView ArticlePubMedGoogle Scholar
- Maragkoudakis PA, Chingwaru W, Gradisnik L, Tsakalidou E, Cencic A: Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection. Int J Food Microbiol. 2010, 141 (Suppl 1): S91-S97.View ArticlePubMedGoogle Scholar
- Salva S, Nunez M, Villena J, Ramon A, Font G, Alvarez S: Development of a fermented goats' milk containing Lactobacillus rhamnosus: in vivo study of health benefits. J Sci Food Agric. 2011, 91 (13): 2355-2362. 10.1002/jsfa.4467.View ArticlePubMedGoogle Scholar
- Salva S, Villena J, Alvarez S: Immunomodulatory activity of Lactobacillus rhamnosus strains isolated from goat milk: impact on intestinal and respiratory infections. Int J Food Microbiol. 2010, 141 (1–2): 82-89.View ArticlePubMedGoogle Scholar
- Villena J, Salva S, Nuñez M, Corzo J, Tolaba R, Faedda J, Font G, Alvarez S: Probiotics for everyone! The novel immunobiotic Lactobacillus rhamnosus CRL1505 and the beginning of Social Probiotic Programs in Argentina. Int J Biotechnol Wellness Industries. 2012, In PressGoogle Scholar
- Wolf DG, Greenberg D, Kalkstein D, Shemer-Avni Y, Givon-Lavi N, Saleh N, Goldberg MD, Dagan R: Comparison of human metapneumovirus, respiratory syncytial virus and influenza A virus lower respiratory tract infections in hospitalized young children. Pediatr Infect Dis J. 2006, 25 (4): 320-324. 10.1097/01.inf.0000207395.80657.cf.View ArticlePubMedGoogle Scholar
- Gentile A, Bardach A, Ciapponi A, Garcia-Marti S, Aruj P, Glujovsky D, Calcagno JI, Mazzoni A, Colindres RE: Epidemiology of community-acquired pneumonia in children of Latin America and the Caribbean: a systematic review and meta-analysis. Int J Infect Dis. 2012, 16 (1): e5-e15. 10.1016/j.ijid.2011.09.013.View ArticlePubMedGoogle Scholar
- Marranzino G, Villena J, Salva S, Alvarez S: Stimulation of macrophages by immunobiotic Lactobacillus strains: influence beyond the intestinal tract. Microbiol Immunol. 2012, 56: 771-781. 10.1111/j.1348-0421.2012.00495.x.View ArticlePubMedGoogle Scholar
- Villena J, Chiba E, Tomosada Y, Salva S, Marranzino G, Kitazawa H, Alvarez S: Orally administered Lactobacillus rhamnosus modulates the respiratory immune response triggered by the viral pathogen-associated molecular pattern poly(I:C). BMC Immunol. 2012, 13: 53-10.1186/1471-2172-13-53.PubMed CentralView ArticlePubMedGoogle Scholar
- Stadnyk AW: Intestinal epithelial cells as a source of inflammatory cytokines and chemokines. Can J Gastroenterol. 2002, 16 (4): 241-246.PubMedGoogle Scholar
- Kawai T, Akira S: Innate immune recognition of viral infection. Nat Immun. 2006, 7 (2): 131-137. 10.1038/ni1303.View ArticleGoogle Scholar
- Takeuchi O, Akira S: MDA5/RIG-I and virus recognition. Curr Opin Immunol. 2008, 20 (1): 17-22. 10.1016/j.coi.2008.01.002.View ArticlePubMedGoogle Scholar
- Takeuchi O, Akira S: Innate immunity to virus infection. Immunol Rev. 2009, 227 (1): 75-86. 10.1111/j.1600-065X.2008.00737.x.View ArticlePubMedGoogle Scholar
- Villena J, Suzuki R, Fujie H, Chiba E, Takahashi T, Tomosada Y, Shimazu T, Aso H, Ohwada S, Suda Y, Ikegami S, Itoh H, Alvarez S, Saito T, Kitazawa H: Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells. Clin Vaccine Immunol. 2012, 19 (7): 1038-1053. 10.1128/CVI.00199-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Hosoya S, Villena J, Shimazu T, Tohno M, Fujie H, Chiba E, Shimosato T, Aso H, Suda Y, Kawai Y, Saito T, Alvarez S, Ikegami S, Itoh H, Kitazawa H: Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells. Vet Res. 2011, 42 (1): 111-10.1186/1297-9716-42-111.PubMed CentralView ArticlePubMedGoogle Scholar
- Hosoya S, Villena J, Chiba E, Shimazu T, Suda Y, Aso H, Saito T, Kitazawa H: Advanced application of porcine intestinal epithelial cells for the selection of immunobiotics modulating toll-like receptor 3-mediated inflammation. J Microbiol Immunol Infect. 2013, 46 (6): 474-478. 10.1016/j.jmii.2012.04.005.View ArticlePubMedGoogle Scholar
- Moue M, Tohno M, Shimazu T, Kido T, Aso H, Saito T, Kitazawa H: Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line. Biochim Biophys Acta. 2008, 1780 (2): 134-144. 10.1016/j.bbagen.2007.11.006.View ArticlePubMedGoogle Scholar
- Frias AH, Vijay-Kumar M, Gentsch JR, Crawford SE, Carvalho FA, Estes MK, Gewirtz AT: Intestinal epithelia activate anti-viral signaling via intracellular sensing of rotavirus structural components. Mucosal Immunol. 2010, 3 (6): 622-632. 10.1038/mi.2010.39.PubMed CentralView ArticlePubMedGoogle Scholar
- Akira S: Pathogen recognition by innate immunity and its signaling. Proc Jpn Acad Ser B Phys Biol Sci. 2009, 85 (4): 143-156. 10.2183/pjab.85.143.PubMed CentralView ArticlePubMedGoogle Scholar
- Akira S: Innate immunity and adjuvants. Philos Trans R Soc Lond B Biol Sci. 2011, 366 (1579): 2748-2755. 10.1098/rstb.2011.0106.PubMed CentralView ArticlePubMedGoogle Scholar
- Meylan E, Tschopp J: Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses. Mol Cell. 2006, 22 (5): 561-569. 10.1016/j.molcel.2006.05.012.View ArticlePubMedGoogle Scholar
- Sen GC, Sarkar SN: Transcriptional signaling by double-stranded RNA: role of TLR3. Cytokine Growth Factor Rev. 2005, 16 (1): 1-14. 10.1016/j.cytogfr.2005.01.006.View ArticlePubMedGoogle Scholar
- Frias AH, Jones RM, Fifadara NH, Vijay-Kumar M, Gewirtz AT: Rotavirus-induced IFN-beta promotes anti-viral signaling and apoptosis that modulate viral replication in intestinal epithelial cells. Innate immunity. 2012, 18 (2): 294-306. 10.1177/1753425911401930.View ArticlePubMedGoogle Scholar
- Feng N, Kim B, Fenaux M, Nguyen H, Vo P, Omary MB, Greenberg HB: Role of interferon in homologous and heterologous rotavirus infection in the intestines and extraintestinal organs of suckling mice. J Virol. 2008, 82 (15): 7578-7590. 10.1128/JVI.00391-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Barro M, Patton JT: Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7. J Virol. 2007, 81 (9): 4473-4481. 10.1128/JVI.02498-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Haller D, Bode C, Hammes WP, Pfeifer AM, Schiffrin EJ, Blum S: Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut. 2000, 47 (1): 79-87. 10.1136/gut.47.1.79.PubMed CentralView ArticlePubMedGoogle Scholar
- Vinderola G, Matar C, Perdigon G: Role of intestinal epithelial cells in immune effects mediated by gram-positive probiotic bacteria: involvement of toll-like receptors. Clin Diagn Lab Immunol. 2005, 12 (9): 1075-1084.PubMed CentralPubMedGoogle Scholar
- Castillo NA, de Moreno de LeBlanc A, MG C, Perdigon G: Comparative study of the protective capacity against Salmonella infection between probiotic and nonprobiotic Lactobacilli. J Appl Microbiol. 2013, 114 (3): 861-876. 10.1111/jam.12074.View ArticlePubMedGoogle Scholar
- Chieppa M, Rescigno M, Huang AY, Germain RN: Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement. J Exp Med. 2006, 203 (13): 2841-2852. 10.1084/jem.20061884.PubMed CentralView ArticlePubMedGoogle Scholar
- Baba N, Samson S, Bourdet-Sicard R, Rubio M, Sarfati M: Selected commensal-related bacteria and Toll-like receptor 3 agonist combinatorial codes synergistically induce interleukin-12 production by dendritic cells to trigger a T helper type 1 polarizing programme. Immunology. 2009, 128 (1 Suppl): e523-e531.PubMed CentralView ArticlePubMedGoogle Scholar
- Christensen HR, Frokiaer H, Pestka JJ: Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. J Immunol. 2002, 168 (1): 171-178. 10.4049/jimmunol.168.1.171.View ArticlePubMedGoogle Scholar
- Drakes M, Blanchard T, Czinn S: Bacterial probiotic modulation of dendritic cells. Infect Immun. 2004, 72 (6): 3299-3309. 10.1128/IAI.72.6.3299-3309.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Weiss G, Rasmussen S, Zeuthen LH, Nielsen BN, Jarmer H, Jespersen L, Frokiaer H: Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism. Immunology. 2010, 131 (2): 268-281. 10.1111/j.1365-2567.2010.03301.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Bass DM: Interferon gamma and interleukin 1, but not interferon alfa, inhibit rotavirus entry into human intestinal cell lines. Gastroenterology. 1997, 113 (1): 81-89. 10.1016/S0016-5085(97)70083-0.View ArticlePubMedGoogle Scholar
- Hulst M, Kerstens H, de Wit A, Smits M, van der Meulen J, Niewold T: Early transcriptional response in the jejunum of germ-free piglets after oral infection with virulent rotavirus. Arch Virol. 2008, 153 (7): 1311-1322. 10.1007/s00705-008-0118-6.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhou R, Wei H, Sun R, Tian Z: Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007, 178 (7): 4548-4556. 10.4049/jimmunol.178.7.4548.View ArticlePubMedGoogle Scholar
- Cario E, Podolsky DK: Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. Infect Immun. 2000, 68 (12): 7010-7017. 10.1128/IAI.68.12.7010-7017.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Galdeano CM, Perdigon G: The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin Vaccine Immunol. 2006, 13 (2): 219-226. 10.1128/CVI.13.2.219-226.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Mohamadzadeh M, Olson S, Kalina WV, Ruthel G, Demmin GL, Warfield KL, Bavari S, Klaenhammer TR: Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization. Proc Natl Acad Sci U S A. 2005, 102 (8): 2880-2885. 10.1073/pnas.0500098102.PubMed CentralView ArticlePubMedGoogle Scholar
- Plantinga TS, van Maren WW, van Bergenhenegouwen J, Hameetman M, Nierkens S, Jacobs C, de Jong DJ, Joosten LA, van't Land B, Garssen J: Differential Toll-like receptor recognition and induction of cytokine profile by Bifidobacterium breve and Lactobacillus strains of probiotics. Clin Vaccine Immunol. 2011, 18 (4): 621-628. 10.1128/CVI.00498-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Wells JM, Rossi O, Meijerink M, van Baarlen P: Epithelial crosstalk at the microbiota–mucosal interface. Proc Natl Acad Sci USA. 2010, 108 ((supple. 1)): 4607-4614. pnas.1000092107: 1-8PubMed CentralPubMedGoogle Scholar
- Abreu MT: Toll-like receptor signalling in the intestinal epithelium: how bacterial recognition shapes intestinal function. Nat Rev Immunol. 2010, 10 (2): 131-144. 10.1038/nri2707.View ArticlePubMedGoogle Scholar
- Es-Saad S, Tremblay N, Baril M, Lamarre D: Regulators of innate immunity as novel targets for panviral therapeutics. Curr Opin Virol. 2012, 2 (5): 622-628. 10.1016/j.coviro.2012.08.009.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.