Figure 4From: Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells Effect of immunobiotic lactobacilli in porcine antigen presenting cells (APCs) from Peyer’s patches co-cultured with porcine intestinal epithelial (PIE) cells. PIE cells were co-cultured with adherent cells from Peyer’s patches and stimulated with Lactobacillus rhamnosus CRL1505 (Lr1505) or L. rhamnosus CRL1506 (Lr1506) for 12 hours. PIE-APCs co-cultures were then challenged with poly(I:C). The mRNA expression of IFN-α, IFN-β, IL-1β, TNF-α, IFN-γ, IL-6, IL-2, IL-12, IL-10 and TGF-β was studied at different time points after challenge. Cytokine mRNA levels were calibrated by the swine β-actin level and normalized by common logarithmic transformation. Values represent means and error bars indicate the standard deviations. The results are means of 3 measures repeated 4 times with independent experiments. The mean differences among different superscripts letters were significant at the 5% level.Back to article page