Skip to main content
Figure 3 | BMC Microbiology

Figure 3

From: Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells

Figure 3

Effect of immunobiotic lactobacilli in porcine antigen presenting cells (APCs) from Peyer’s patches. (A) Monocultures of adherent cells from Peyer’s patches were stimulated with Lactobacillus rhamnosus CRL1505 (Lr1505) or L. rhamnosus CRL1506 (Lr1506) for 12 hours and then challenged with poly(I:C). The mRNA expression of IFN-α, IFN-β, IL-1β, TNF-α, IFN-γ, IL-6, IL-2, IL-12, IL-10 and TGF-β was studied after 12 hours of stimulation. Cytokine mRNA levels were calibrated by the swine β-actin level and normalized by common logarithmic transformation. (B) In addition, expression of MHC-II and CD80/86 molecules as well as intracellular levels of IL-1β, IL-10, IFN-γ and IL-10 were studied in the three populations of APCs within adherent cells defined with CD172a and CD11R1 markers. Values represent means and error bars indicate the standard deviations. The results are means of 3 measures repeated 4 times with independent experiments. The mean differences among different superscripts letters were significant at the 5% level.

Back to article page