Comparative transcriptomics in Yersinia pestis: a global view of environmental modulation of gene expression
- Yanping Han†1,
- Jingfu Qiu†1,
- Zhaobiao Guo1,
- He Gao1,
- Yajun Song1,
- Dongsheng Zhou1Email author and
- Ruifu Yang1Email author
© Han et al; licensee BioMed Central Ltd. 2007
Received: 02 June 2007
Accepted: 29 October 2007
Published: 29 October 2007
Environmental modulation of gene expression in Yersinia pestis is critical for its life style and pathogenesis. Using cDNA microarray technology, we have analyzed the global gene expression of this deadly pathogen when grown under different stress conditions in vitro.
To provide us with a comprehensive view of environmental modulation of global gene expression in Y. pestis, we have analyzed the gene expression profiles of 25 different stress conditions. Almost all known virulence genes of Y. pestis were differentially regulated under multiple environmental perturbations. Clustering enabled us to functionally classify co-expressed genes, including some uncharacterized genes. Collections of operons were predicted from the microarray data, and some of these were confirmed by reverse-transcription polymerase chain reaction (RT-PCR). Several regulatory DNA motifs, probably recognized by the regulatory protein Fur, PurR, or Fnr, were predicted from the clustered genes, and a Fur binding site in the corresponding promoter regions was verified by electrophoretic mobility shift assay (EMSA).
The comparative transcriptomics analysis we present here not only benefits our understanding of the molecular determinants of pathogenesis and cellular regulatory circuits in Y. pestis, it also serves as a basis for integrating increasing volumes of microarray data using existing methods.
Yersinia pestis is the etiological agent of plague, alternatively growing in fleas or warm-blood mammals . Fleas acquire this organism via blood meal from a bacteremic mammal, usually a rodent. To produce a transmissible infection, Y. pestis colonizes the flea midgut and forms a biofilm in the proventricular valve optimally at 20 to 26°C, blocking its normal blood feeding . Human beings are occasionally infected by directly contacting infected animals or by being bitten by the blocked fleas. Thus, Y. pestis must experience a temperature shift during the transmission process between rodents, fleas, and humans. It is considered a facultative intracellular pathogen. After the initial subcutaneous invasion, the bacteria migrate into the regional lymph nodes via the subcutaneous lymph vessel. Most of the organisms that invade the lymph nodes are engulfed and killed by the polymorphonuclear leukocytes (PMNs) that are attracted to invasion sites in large numbers. However, a few bacilli are taken up by tissue macrophages, providing a fastidious and unoccupied niche for Y. pestis to synthesize virulence determinants . Residence in this niche also facilitates the bacteria's resistance to phagocytosis [4, 5]. The moiety escaped from macrophages can multiply outside of host cells and eventually cause systemic infection. The hypothesized prevailing conditions of phagolysosomal microenvironments include acidic pH, oxidative stress, iron scavenging, nutrition limitation, and killing or inhibiting activities of antibacterial peptides. To survive these stressful environments, Y. pestis likely makes appropriate adaptive responses, primarily reflected by the transcriptional changes of specific sets of genes.
A DNA microarray is able to determine simultaneous changes in all the genes of a cell at the mRNA level . We and others have measured the gene expression profiles of Y. pestis in response to a variety of stimulating conditions (stimulon analysis), including temperature alteration tolerance [7–9], increased osmolarity , ion deficiency , antibiotic treatment [12, 13], oxidative and acidic stresses , antibacterial peptide treatment  and nutrition limitation. We also identified the regulons controlled by each of the regulatory proteins, Fur , PhoP , OmpR, and OxyR, by comparing the gene expression patterns of the mutant transcriptional regulator with that of its parental strain. In order to acquire more regulatory information, all available microarray data of Y. pestis including those published signature expression profiles [8–13, 15] were collected and subjected to clustering analysis, which infers functionality to the clusters of co-regulated genes.
The transcriptional and genomic information gleaned from coordinately regulated genes was also used to computationally search for potential operons (operon prediction) and cis-acting DNA regulatory motifs (motif discovery). Some important findings were further verified by biochemical experiments, including RT-PCR and gel shift assays. This analysis provides an opportunity to gain a global view of environmental modulation of gene expression patterns in Y. pestis.
Results and Discussion
Comprehensive analysis of large sets of microarray expression data is useful to dissect bacterial adaptation to various environments and to understand bacterial gene transcriptional regulation [16, 17]. For example, Kendall and his colleagues have compared the general responses of Mycobacterium tuberculosis induced by a variety of different in vitro conditions (low pH, low nutrients, nitrogen, oxygen stress, stationary phase, and nutrition starvation) . After the determination of the CsrA, SlyA, and PhoPQ regulons in Samonella typhimurium, the relevant regulon members are monitored to define the synergetic or antagonistic roles between these three regulators in cell infection models .
Recently, many signature expression profiles of Y. pestis have been reported [7–13, 15, 19–21]. All the microarray expression data from our laboratory were analyzed using standardized microarray procedures such that they are suitable for comprehensive analysis. Comparative transcriptomics analysis presented here can be used to mine the regulatory information from these available microarray data, providing an opportunity to gain a global view on environmental modulation of gene expression in Y. pestis. This analysis provides an additional dividend towards the transcriptional regulatory networks of Y. pestis.
Virulence genes in response to multiple environmental stresses
In this work, 25 expression profiles of Y. pestis were collected for further integration. We hypothesize that the stress conditions used in these experiments will be encountered by this bacterium during its infection and life cycle. The data supported the notion that Y. pestis has evolved its ability to coordinately regulate a wide set of genes to survive a wide range of environmental perturbations. Almost all of the known virulence genes were active in the stress responses. Thus, identification of the expression patterns of virulence genes upon a wide set of environmental changes will provide a reference to screen for uncharacterized genes that shown the same differential gene expression under the same stressful conditions.
As described previously, expression profiles of Y. pestis showed that almost all the putative virulence genes of Y. pestis were differentially regulated upon temperature alteration [7–9]. Our data showed that Y. pestis known virulence genes also respond to other environmental stresses besides temperature shift (Figure 1). For example, the hemin storage locus, hmsHFRS , was repressed by temperature upshift, high osmolarity, nutrition limitation, and streptomycin treatment. The ymt gene encoding Yersinia murine toxin  was also regulated by temperature upshift and streptomycin treatment.
Y. pestis synthesizes several antiphagocytic factors, including F1 capsular antigen , pH6 antigen  and Yersinia outer proteins (Yops) . Expression of Yops was regulated by temperature alteration, increased osmolarity, and nutrition deficiency under normal Ca2+ condition. These data suggest that the low-calcium response of type III secretion system (T3SS) appears to be triggered at the mRNA level by other environmental cues in addition to temperature upshift and Ca2+ limitation. F1 capsular antigen is expressed much more at 37°C than at 26°C . pH6 antigen (PsaA), encoded by the chromosomal psaA gene, expresses in vitro between pH 5 and 6.7 at 35 to 41°C , or when bacteria live within phagocytic phagolysome . The psaEFABC operon encodes a chaperone/usher pathway involved in the secretion and assembly of pH6 antigen as a polymer (fimbriae) on the surface of Y. pestis in macrophages [28, 30]. PsaE is thought to be a positive regulator of the psaABC locus and is required for maximal expression of the pH6 antigen . A recent study showed that the psaEFABC locus is regulated by RovA . The microarray data showed that the F1 operon was upregulated upon temperature upshift, low pH medium, oxidative stress, low Mg2+, and nutrition deficiency, while the psaEFABC locus was induced by temperature alteration, acid stress, low Mg2+, nutrition limitation, high salinity and hyperosmotic stress. It is reasonable to assume that synergetic operation of complicated microenvironments within mammalian hosts account for the full expression of these two loci.
Prediction of operons from microarray data
Operon prediction is the first step toward elucidating gene regulation and reconstructing regulatory networks. Most approaches for prokaryotic operon prediction were developed on the basis of genomic and/or phylogenetic information . For these methods, training with experimental information of known operons is required to generate the predictors. However, little experimental data of operon structure is currently available for Y. pestis. To predict the potential operons of Y. pestis, we attempted a method that incorporated the empirical correlation coefficient from microarray expression data with the genomic annotation data, including gene orientation, intergenic distance, functional similarity, and intra-genome conservation.
Stress-responsive operons predicted from microarray expression data
Stress-responsive operons in Y. pestis predicted from microarray expression data
Potential operon (r value)
Putative or predicted function
Iron uptake or heme synthesis
yfeABCD operon* (r > 0.91)
Transport/binding chelated iron
hmuRSTUV operon (r > 0.90)
ysuJIHG* (r > 0.95)
sufABCDS* (r > 0.90)
Iron-regulated Fe-S cluster assembly?
YPO1854-1856* (r > 0.97)
Iron uptake or heme synthesis?
tauABCD operon (r > 0.90)
ssuEADCB operon (r > 0.97)
ssu operon 
cys operon (r > 0.92)
YPO1317-1319 (r > 0.97)
YPO4109-4111 (r > 0.90)
Urea uptake and urease activation
ure operon* (r > 0.96)
ure [58, 59]
Stress response and adaptation
dnaKJ operon (r = 0.97)
Chaperones, chaperonins, heat shock
dnaKJ [60, 61]
hslUV operon (r = 0.97)
Adaptions and atypical conditions
katY-cybCB operon* (r > 0.90)
Detoxification and electron transport
psp operon (r > 0.90)
Adaptions and atypical conditions
psp operon 
rps-rpm-rpl operon (r > 0.90)
Ribosomal protein synthesis and modification
rps-rpm-rpl operon 
sdh-suc operon* (r > 0.92)
Tricarboxylic acid cycle
cyo operon (r > 0.94)
nap operon (r > 0.94)
nap operon 
atp operon (r > 0.93)
ATP-proton motive force
ace operon* (r > 0.90)
nuo operon* (r > 0.92)
nuo operon 
Degradation and transport/binding of amino acids
pro operon* (r > 0.92)
Transport/binding amino acids and amines
ast operon (r > 0.90)
Degradation of amino acids
gln operon* (r > 0.91)
Transport/binding amino acids and amines
YPO1994-1996* (r > 0.98)
YPO0881-0884 (r = 0.99)
Chemotaxis and mobility?
YPO1087-1088 (r = 0.99)
Phage-related functions and prophage
YPO0623-0628* (r > 0.94)
mur operon (r > 0.95)
Murein sacculus and peptidoglycan
idn operon (r = 0.96)
Degradation of carbon compounds
fad operon* (r = 0.95)
Degradation of small molecule
glg operon (r > 0.90)
Synthesis and modification of cytoplasmic polysaccharides
glg operon 
YPO3838-3839 (r = 0.92)
YPO0408-0409* (r = 0.97)
YPO1516-1517 (r = 0.90)
YPCD1.15c-1.17c (r > 0.98)
yscGHIJK operon* (>0.90)
YPPCP1.08c-1.09c (r = 0.97)
Verification of predicted operons by RT-PCR
This analysis predicted a total of 39 operons in Y. pestis. The advantage of our strategy is that a high accuracy rate would be achieved by integrating microarray experimental data with genomic information. Although there are many bioinformatics tools available for predicting operons, results presented here demonstrated a new reliable strategy for operon prediction and verification, which will be helpful for functional studies. However, only a small number of operons could be predicted, due to the fact i) that only stress-responsive genes were included for this analysis, and ii) that a lot of contiguous gene pairs passed the criteria, but incomplete array data made it difficult to define the border of their primary mRNA transcripts (for example, the extension of a predicted operon, YPO0881-0882 to YPO0881-0884, was demonstrated by RT-PCR as shown in Figure 2d)
Functional inference of clustering, uncharacterized genes
Clustering analysis and functional classification of co-expressed gene clusters
Clustering analysis of the whole microarray dataset was analyzed and four distinct clusters of co-expressed genes, cluster I, II, III, and IV, were identified (Figure 3).
Cluster I consisted of more than 70 genes, most of which are functionally related to biosynthesis of ribosomal proteins. The ribosome is the factory of protein synthesis, and it determines the capacity of the cell to synthesize proteins, thus determining the growth rate of the bacteria. Since most of the members in Cluster I were down-regulated in response to a temperature shift from 26 to 37°C, high osmolarity, Mg2+ limitation, nutrition deficiency, and antibiotics treatment, Y. pestis appeared to slow its growth rate under these conditions (see Additional File 1).
Classification of the gene members of the cluster II in Figure 3
Category A: Proven
Inorganic iron and manganese binding/transport system
Inorganic iron transport system
Siderophore-dependent Yersiniabactin biosynthesis and transport
Heme transport system
Putative siderophore ABC-transporter
Siderophore biosynthetic enzyme system
Category B: Putative
Putative iron ABC transporter
putative siderophore biosysnthesis protein
putative TonB-dependent outer membrane receptor
Putative hydroxamate-type ferri siderophore receptor
Putative ferric siderophore receptor (pseudogene)
Category C: Hypothetical
Fe-S cluster assembly
Putative exported protein
Zinc, lead, cadmium and mercury transporting ATPase
Putative membrane protein
Putative exported protein
Putative ABC transporter (ATP-binding protein)
Putative membrane or exported protein
Sulfur is one of the nutrients necessary for bacterial life. Genes responsible for sulfur uptake and utilization constitute the cys regulon in Gram-negative bacteria . Cluster III contains members of the cy s regulon, including tauABCD, ssuEADCB, cysPUWAM, and sbp1. These genes were regulated by most of the environmental stresses under study, implying that sulfur metabolism might play important roles in the adaptation of Y. pestis to various environmental perturbations. Two genomic loci, YPO1316-1319 and YPO4108-4111, are also included in this sulfur-metabolism-related cluster. Most of the gene products within these two loci were annotated as conserved hypothetical proteins. These two genomic loci might have functions related to sulfur metabolism.
As shown in cluster IV in Figure 3, sdhCDAB and sucABCD involved in tricarboxylic acid cycle had an expressional pattern similar to that of nuoA-N and cyoABCDE involved in aerobic respiration. The microarray data showed that these energy metabolism-related genes were down-regulated upon heat shock, high osmolarity, Mg2+ limitation, and streptomycin treatment, but they were upregulated upon chloramphenicol treatment. These results indicated a general retardation of energy generation in Y. pestis might occur in response to these suboptimal growth conditions.
Prediction of regulatory DNA motifs from clustering data
Functionally related members within a cluster of co-expressed genes are likely to be regulated by similar mechanisms; sometimes expression of these genes is even controlled by a single regulatory protein. Promoter DNA sequences containing short (5–20 bp) and relatively conserved regulatory DNA motifs represent the predominant contact sites with the regulatory protein. In this study, the promoter-proximate DNA sequences were collected from each cluster of co-expressed genes. The subsequent motif discovery analysis indicated the presence of DNA motifs that resembled the experimentally proved Fur, PurR, CRP, and Fnr boxes of E. coli and other bacteria [42–46], respectively.
Computational discovery of regulatory DNA motifs
Functionally related members of a cluster of co-expressed genes are likely regulated by similar mechanisms, and even share common cis-regulatory DNA elements within their promoter DNA regions. The presence of a motif-like sequence within the upstream region of a gene suggests that it is likely a direct target of the corresponding regulatory protein.
Motif discovery for the clustering genes
Genes or operons for motif discovery
Strict consensus of known TF-like box (See also Figure 4)
Hits of consensus
rps-rpm-rpl operon, rpsLG, rpsF-priB-rpsR-rplI, purEK, ruvCAB, rpsB, rplMI, rpsP-rimM-trmD-rplS, nusA-infB and rluC
PurR-like box: 5' ACGCAATCGTTTTCNT 3'
rps-rpm-rpl operon, purEK, ruvCAB, rpsB, rpsP-rimM-trmD-rplS, nusA-infB and rluC
hmuRSTUV, YPO0682, YPO0778, YPO0988, YPO1003, YPO1011, ysuFJIHG, YPO1735, YPO1854-YPO1856, irp2-irp1-ybtUTE, ybtPQXS, YPO2163, sufABCDSE, yfeABCD, nrdHIEF, yfuABC, YPO3086, YPO3339, yacK, yhhN-zntA and YPO4022
Fur-like box: 5' TGATAATGATTATCATTATCA 3'
hmuRSTUV, YPO0682, YPO0988, YPO1011, ysuFJIHG, YPO1735, YPO1854-YPO1856, irp2-irp1-ybtUTE, YPO2163, sufABCDSE, yfeABCD, nrdHIEF, yfuABC, YPO3086, YPO3339, yacK, yhhN-zntA and YPO4022
cysB, ssuEADCB, cysK, YPO3541, YPO1517-YPO1516, YPO1316, YPO1317-YPO1319, fliY, sbp1, tauABCD, YPO0186, YPO2360, YPO3010, cysP, YPO4112, YPO4108, ilvC, YPO2315 and gntT
Fnr-like box: 5' TGAN6TCAA 3'
ssuEADCB, cysK, YPO1517-YPO1516, YPO1317-YPO1319, fliY, sbp1, tauABCD and gntT
sdhCDAB-sucABCD, nuoA-N, cyoABCDE, purB, pta, kbl-tdh, metG, aceE, cysJIH, acnB, murEFXD, YPO1523, gph, trpS, pepD, accBC, mutS, ppc, cydAB, fadBA, fadL, fumA, mdh, oppABCDF, treBC, manX, napFDABC and frdABCD
Fnr/Crp-like box: 5' TGANNNNNNTCA 3' ArcA-like box:5' GTTAATTAATGT 3'
sdhCDAB-sucABCD, pta, kbl-tdh, gph, pepD, mutS, cydAB, fadBA, fumA, oppABCDF, treBC, manX and frdABCD acnB, pepD, mutS, mdh, oppABCDF, manX and frdABCD
A box sequence (5'-TGA N6TCA-3') was strictly present in the promoter regions of 14 genes in cluster IV. It is a part of the binding boxes of CRP  and Fnr . Previous DNA-binding studies showed that CRP bound to exactly the same sequence as that recognized by Fnr . The ArcA regulator can recognize a relatively conservative sequence (5'-GTTAATTAA-3') . An ArcA-box-like sequence (5'-GTTAATTAATGT-3') was found in the upstream sequence of 7 genes in cluster IV (Table 3).
EMSA analysis of Fur binding
The comprehensive transcriptomics analysis benefits our understanding of the molecular determinants of bacterial pathogenesis and cellular regulatory circuits. Our study gave some hints to the possible function of uncharacterized genes and regulatory elements of Y. pestis such as operons and DNA regulatory motifs. This kind of analysis would provide an opportunity to gain a global view of environmental modulation of gene expression patterns in Y. pestis, which also serve as a basis for integrating increasing volumes of microarray data using existing methods.
Collection of microarray expression data
Designs for expression profiling of Y. pestis
Vegetative growth temperatures
Shift from 26°C to 37°C for 3 hr
Shift from 37°C to 45°C for 10 min
Shift from 37°C to 10°C for 1 hr
Treatment with 0.5 M sorbitol for 20 min
Treatment with 0.5 M NaCl for 20 min
Treatment with 1 mM H2O2 for 30 min at 26°C
Mild acid stress
Shift from pH7.2 to pH 5.5 for 10 min
Growth under 10 μM Mg2+
Treatment with 100 μM of 2, 2'-dipyridyl for 30 min
26°C cell cultures
37°C cell cultures
Minimal versus rich medium
TMH versus BHI medium
Growth to an A620 nm of approximately 0.8
Growth to an A620 nm of approximately 2.0
Exponential versus stationary phase
Growth in TMH medium
Growth in BHI medium
10× MIC concentration of antibiotics for 30 min
37°C cell cultures
37°C cell cultures
37°C cell cultures
26°C cell cultures
phoP mutant versus Wide-type (WT) strain
Growth at pH5.5
Growth under 10 μM Mg2+
fur mutant versus WT strain
26°C cell cultures with addition of 40 μM of FeCl3 for 30 min
37°C cell cultures with addition of 40 μM of FeCl3 for 30 min
oxyR mutant versus WT strain upon exposure to 1 mM H2O2 for 30 min at 26°C
ompR mutant versus WT strain
Upon exposure to 0.5 M sorbitol for 20 min
Upon exposure to 0.5 M NaCl for 20 min
For microarray hybridization, two independent bacterial cultures for each growth condition were prepared as biological replicates for RNA isolation. Three separate labeled probes were made for each RNA preparation as technical replicates. Pairwise comparisons were made using dye swaps to avoid labeling bias.
Whole-genome DNA microarray spotted with 4,005 ORFs was used in our study as described in our previous publications. The data filtering, normalization and significance analysis were done as described previously [9, 10]. After filtering the corrupted or suspicious spots during the image analysis phase, expression data of approximately 50%~80% of Y. pestis genes on array are available in each array experiment. All the transcriptome data were collected and displayed as a 4,005 × 25 matrix for all the transcriptional changes of 4,005 genes of Y. pestis in response to 25 environmental perturbations (see Additional File 2). All the microarray data was deposited in Gene Expression Omnibus (GEO accession number GSE9279).
To measure similarities in gene transcriptional regulation, we calculated the correlation coefficient (r) of neighboring genes between mRNA transcriptional patterns under multiple environmental conditions. We then further focused our analysis to those genes across all arrays that were differentially regulated in at least five experiments. All the analysis was based on the use of log2-ratio of expression values: let xi and yi be the log-ratio of fluorescence intensities for a pair of neighboring genes in experiment i, where n is the number of experiments; n = 25. For each pair of genes, we calculated the Pearson correlation coefficient, r, between mRNA expression profiles as follows:
Genes were assigned to a potential operon if they met the following criterions: i) the correlation coefficient, r, of neighboring genes was greater than 0.90; ii) they are transcribed in the same direction and are located on the same strand; iii) intergenic distance between two adjacent genes was shorter than 300 bp and iv) they are functionally linked based on the genomic annotation and/or related publications.
Verification of predicted operons by RT-PCR
According to the microarray data, genes tested in operon validation were significantly regulated under the growth temperatures of 26°C, 37°C and 45°C. Therefore, RNA was extracted from cells grown at these three temperatures, as done in microarray experiments. Primer pairs were designed for each adjacent gene studied, such that a PCR amplicon across the intergenic region of the two genes would be produced when genomic DNA was used as template (see Additional File 3). cDNA was prepared by reverse transcription with 5 μg of RNA, 200 U of Superscript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) and 3 μg of random hexamer primers. A sample of the resulting cDNA (5 μl) was used for PCR amplification. PCR products were analyzed by agarose gel electrophoresis. To ensure that there was no contamination of genomic DNA, negative controls were performed using RT products with no addition of reverse transcriptase. Reactions containing primer pairs without cDNA template were also included as blank controls.
Genes were clustered according to their expression patterns in the twenty-five different experiments using Cluster 3.0 software . Before clustering, genes with expression data in less than three experiments were removed in order to limit the effects of missing values in the clustering analyses. Of the original 4005 probes, the remaining 3339 probes were used for further clustering analysis. For the original and the filtered data, self-organizing map (SOM) program first ran on the whole microarray dataset to cluster the genes based on the similarity of their expression profiles. The output files were then processed by hierarchical clustering by using the average linkage method. Correlation coefficients of more than 0.6 were arbitrarily extracted and visualized with TreeView  to graphically display the data by coloring each cell on the basis of the fluorescence ratio. Cells with log ratios of 0 (no change in gene expression) are colored in black; increasing values of positive and negative log ratios are labeled with increasing intensities of red and green, respectively.
Discovery of regulatory DNA motifs
For each cluster of co-expressed genes, neighboring genes were grouped into potential operon structures as described above. Promoter-proximate DNA sequences (400 bp upstream to 50 bp downstream of the start codon of each single-gene transcriptional unit, or the first gene of each operon) were retrieved from the genomic sequence of Y. pesti s 91001 with the retrieve-sequence tool . Collections of promoter sequences from co-expressed genes within each cluster were searched for potential regulatory DNA motifs with the multiple expectation maximization for motif elicitation (MEME) program .
10–30 bases of a single motif and at least 3 distinct motifs in each run were specified. The first two significant (low E-value) motifs except AT-rich motifs were chosen for further analysis. The symmetrical or dyad elements were preferentially selected. Then, the sequence logos were built by compilation of the conserved sequences detected in the analysis for each motif with the WebLogo program . Finally, PSSM for each DNA motif were built with the consensus and convert-matrix tools .
Gel mobility shift analysis of Fur binding
The entire coding region of the fur gene of Y. pestis strain 201 was cloned into pET28a (Novagen, San Diego, CA). Recombinant plasmids encoding a His-Fur fusion protein were transformed into Escherichia coli BL21 (DE3) cells. Expression of His-Fur was induced by addition of isopropyl-β-D-thiogalactoside (IPTG). The recombinant protein was purified under native conditions with a QIAexpressionist™ Ni-NTA affinity chromatography (Qiagen, Valencia, CA). The purified, eluted protein was concentrated to approximately 0.3 mg ml-1 with the Amicon Ultra-15 (Millipore, Billerica, MA). The purity of His-Fur protein was verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Primers were designed to amplify the 400–500 bp promoter-proximate region extending upstream from the start codon of each first gene of the operons tested (Table S2 for list of oligonucleotide primers used in this study). EMSA was performed using the Gel Shift Assay Systems (Promega, Madison, WI). The DNA was 5'end-labeled using [γ-32P] ATP and T4 polynucleotide kinase. DNA binding was performed in a 10 μl reaction volume containing: binding buffer [100 μM MnCl2, 1 mM MgCl2, 0.5 mM DTT, 50 mM NaCl, 10 mM Tris-HCl (pH 7.5) and 0.05 mg ml-1 poly-(dI-dC)], labeled DNA (1000–2000 c.p.m. μl-1), and increasing amounts of His-Fur protein. We included two control reactions: one contained the specific DNA competitor (unlabeled promoter DNA), while the other was the nonspecific protein competitor [rabbit anti-F1-protein polyclonal antibody (IgG)]. After incubation at room temperature for 30 minutes (min), the products were loaded onto a native 4% (w/v) polyacrylamide gel and electrophoresed in 0.5 × Tris-borate (TB) buffer containing 100 μM MnCl2 for 30 min at 220 V. Radioactive species were detected by autoradiography.
We thank Dr. Chen Chen and his co-workers from Chinese Academy of Sciences for their assistance during the course of this work. This study was funded by the National Natural Science Foundation of China (contract no. 30430620). The English writing of the manuscript was polished by International Science Editing, Ireland.
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