Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B viruses

Table 5 Summary of clinical performance of our multiplex assay and commercial multiplex kit compared to reference simplex assays

Virus (target)	Our Multiplex assay				Sensitivity TP/TP + FN	Specificity TN/FP + TN	Accuracy TP + TN/TP+ TN + FP + FN	Positive predictive values TP/TP + FP	Negative predictive values TN/TN + FN	Kappa index	P-value (McNemar Test)
Virus (target)	TP	TN	FN	FP	Sensitivity TP/TP + FN	Specificity TN/FP + TN	Accuracy TP + TN/TP+ TN + FP + FN	Positive predictive values TP/TP + FP	Negative predictive values TN/TN + FN	Kappa index	P-value (McNemar Test)
SARS CoV-2	17	26	1	0	0.944	1	0.977	1	0.962	0.953	1.00
IAV	15	26	1	0	0.937	1	0.977	1	0.962	0.949	1.00
IBV	10	26	0	0	1	1	1	1	1	1.000	1.00
Total	42	26	2	0	0.954	1	0.971	1	0.928	0.940	0.50
	Commercial kit
SARS CoV-2	16	26	2	0	0.888	1	0.954	1	0.928	0.904	0.50
IAV	15	26	1	0	0.937	1	0.977	1	0.962	0.949	1.00
IBV	10	26	0	0	1	1	1	1	1	1.000	1.00
Total	41	26	3	0	0.931	1	0.957	1	0.896	0.910	0.25

TP, True Positive; TN, True Negative; FN, False Negative; FP, False Positive; IAV, Influenza A Virus; IBV, Influenza B Virus

ISSN: 1471-2180