Table 4 Comparison of our multiplex assay results versus with commercial multiplex kit in clinical samples
Virus | Clinical samples confirmed by singleplex RT-qPCR assay | Agreement | ||||||
|---|---|---|---|---|---|---|---|---|
Commercial multiplex kit | Our multiplex assay | |||||||
Number | Number | Ct mean | Number | Ct mean | ||||
Positive | Negative | Positive | Negative | positive | Negative | |||
SARS-CoV-2 | 18 | 26 | 16 | 26 | 20.34 | 17 | 26 | 21.62 |
IAV | 16 | 26 | 15 | 26 | 21.19 | 15 | 26 | 22.56 |
IBV | 10 | 26 | 10 | 26 | 17.13 | 10 | 26 | 17.16 |
Total | 44 | 26 | 41 | 26 | 19.55 | 42 | 26 | 20.44 |