Altered motility of Caulobacter Crescentus in viscous and viscoelastic media
© Gao et al.; licensee BioMed Central. 2014
Received: 20 August 2014
Accepted: 11 December 2014
Published: 24 December 2014
Motility of flagellated bacteria depends crucially on their organelles such as flagella and pili, as well as physical properties of the external medium, such as viscosity and matrix elasticity. We studied the motility of wild-type and two mutant strains of Caulobacter crescentus swarmer cells in two different types of media: a viscous and hyperosmotic glycerol-growth medium mixture and a viscoelastic growth medium, containing polyethylene glycol or polyethylene oxide of different defined sizes.
For all three strains in the medium containing glycerol, we found linear drops in percentage of motile cells and decreases in speed of those that remained motile to be inversely proportional to viscosity. The majority of immobilized cells lost viability, evidenced by their membrane leakage. In the viscoelastic media, we found less loss of motility and attenuated decrease of swimming speed at shear viscosity values comparable to the viscous medium. In both types of media, we found more severe loss in percentage of motile cells of wild-type than the mutants without pili, indicating that the interference of pili with flagellated motility is aggravated by increased viscosity. However, we found no difference in swimming speed among all three strains under all test conditions for the cells that remained motile. Finally, the viscoelastic medium caused no significant change in intervals between flagellar motor switches unless the motor stalled.
Hyperosmotic effect causes loss of motility and cell death. Addition of polymers into the cell medium also causes loss of motility due to increased shear viscosity, but the majority of immobilized bacteria remain viable. Both viscous and viscoelastic media alter the motility of flagellated bacteria without affecting the internal regulation of their motor switching behavior.
Many gram-negative bacteria depend on appendages such as flagella, pili, and stalks for successful colonization of their environment. It is well known that the mechanical environment can trigger changes in gene expression and biochemical signaling, and subsequently, induce a functional response from the cells . Studies also have shown, however, some aspects of the mechanical regulation are independent of feedback from genetic or biochemical signaling pathways. Examples include the motor’s sensitivity to load in E. coli, and the flicking motion of a uni-flagellated bacterium as it changes swimming direction from backward to forward, caused by a buckling instability in the hook ,. These recent findings motivate testing of other scenarios whereby mechanical properties of the medium directly dictate or alter motility-related functions of a motile bacterium. Such testing requires a convenient, mechanical platform upon which more refined studies may be designed, and through this line of study certain properties of mechanical origin may be sorted out from others due to genetic regulation or biochemical signaling.
Numerous chemical agents have been utilized experimentally to alter the mechanical environment of bacteria, especially impacting their motility. Studies performed decades ago explored the effects of elevated viscosity on the swimming speed of several species of bacteria, using extremely high molecular weight polymers such as methylcellulose and polyvinylpyrrolidone (PVP) -. It was then recognized that the “gel-like”, or weakly elastic nature of these polymers accounted for enhanced motility of flagellated bacteria -. Another study shows that for Vibrio alginolyticus, the swimming speed of a mutant strain (YM4) expressing only a polar flagellum decreases with increased viscosity in PVP containing media, whereas the strains expressing lateral flagella increase swimming speed with viscosity and attain a peak speed at around 5 cP .
From the polymer rheology perspective, the balance between the viscous and elastic effects is highly dependent on the frequency of the mechanical perturbance, which is periodically exerted by the rotating flagella and cell body ,. The so-called frequency-dependent viscoelasticity is generally dictated by polymer size, concentration, and network entanglement ,. It is therefore most informative to characterize the mechanical effects of polymers of well-defined structure and size on bacterial motility. Our choice for this study is the inert polyethylene glycol (PEG) or polyethylene oxide (PEO) of linear structure and with a wide range of sizes available. PEG and PEO are nearly identical polymers, but differentially termed depending on whether the molecular weight is below or above 100,000 daltons.
Mechanical inhibition of flagellar motor rotation, due to elevated medium viscosity or proximity to surfaces, is also known to trigger swarmer-cell differentiation, which is a key step in pathogenesis in some bacterial species -. Specifically, recent work on Caulobacter crescentus shows that jamming of the flagellum rotation caused by pili-mediated surface contact or aggregation of cells induced by polymeric crowding agents triggers a just-in-time secretion of surface adhesins . In light of this finding, we choose Caulobacter crescentus as a model species to explore for effects on motility caused by mechanical inhibitions that are distinct in nature and perhaps sensitive to the type and molecular size of viscous agents used.
Caulobacter crescentus is a gram-negative, α-proteobacterium often found in nutrient-poor, freshwater environments -. It has a dimorphic life cycle, spending time both as a non-replicating, motile swarmer cell and as a replicating, sessile, stalked cell. During the swarmer stage of its life, it is uni-flagellated. The rotation of its 5–10 μm long flagellum is entirely responsible for moving its 1–2 μm long crescent-shaped body. The swarmer bacterium eventually matures into a sessile cell, characterized by a long, thin, and rigid cell envelope extension called a stalk , which sticks to a nearby surface via an elastic, polysaccharide-based, adhesive holdfast -. This attachment process is facilitated by their type-IV pili, which extend a few μm from the same pole as the flagellum ,, and can retract upon sticking to a surface, or binding of a bacteriophage .
Results and discussion
Our study was designed to test how motility of uni-flagellated bacteria is affected by certain physical characteristics of the medium. Specifically, we chose polyethylene glycol and polyethylene oxide of selected molecular weights, mixing them into the cell medium in order to examine how changes in polymer size, concentration, and solution viscosity affect bacterial motility. We then compared changes in motility and viability of the three selected strains in a PEG/PYE medium in contrast to cells in glycerol/PYE medium. Finally, we delved into measurements of flagellum motor switching in order to detect subtle changes to its biochemical regulation, if any, caused by the test viscous agent.
Loss of motility attributable to apparent viscosity of polymer matrix
Measured viscosity of cell media containing listed polymers
PEG 4000 (%)
PEG 35000 (%)
PEO 400000 (%)
Percentage of motile swarmer cells fell linearly as a function of viscosity
Among the three strains, the wild-type cells lost motility more steeply than the other two strains in both types of media as the viscosity increased. This difference suggests a specific role of pili on the loss of motility, which might be caused by direct interaction between the flagellum and pili that are known to be at the same pole of a C. crescentus swarmer cell. Such an interaction might be weaker and more transient at a lower viscosity, when the flagellum rotates with a higher speed. A faster rotating flagellum is expected to move past the surrounding pili faster, and perhaps less likely to get entangled, trapped, and stalled.
We observed no difference in the percentage of cells losing motility between the two Δpilin strains, with and without motor switching, in either the viscous or viscoelastic medium. This result suggests that the loss of motility, perhaps due to jamming or stalling of the motor, does not depend on motor switching mechanics. It also implies that, while increasing mechanical load on the flagellum can stall or jam the motor, the effect does not appear to be coupled to the switching behavior. Additional experiments were later designed to measure the motor switching behavior while varying the mechanical load, in order to further test this implication.
Significant cell death occurred at high glycerol levels but not in PEG/PEO media of comparable viscosity
We performed a specific test to see whether the loss of motility at high viscosity was reversible using both Δpilin strains. We first mixed swarmer cells with 48% glycerol by mass, and noted that within minutes all cells stopped moving. We then diluted the mixture 1:1 with cell medium, so that the cells were eventually in 24% glycerol with a much reduced viscosity of 1.8 cP. Despite the expectation that over 60% cells would be motile based on Figure 3 for both strains if they were exposed to only 24% glycerol, all the cells that received a shock of 48% glycerol for a few minutes and then were reduced to 24% glycerol remained non-motile over a 30-minute observation period. We conclude that the loss of motility due to elevated levels of glycerol is irreversible.
Previous work by others had shown that at an elevated level of ~50% or higher, glycerol caused the death of E. coli, due to severe osmotic stress or dehydration . On the other hand, it is a widely accepted practice to store bacterial specimens at −80°C in relatively low concentrations of ~15-25% glycerol, in order to prevent ice crystal formation and rapid water loss during the freeze and thaw process . Although we found no similar studies for C. crescentus, the findings for E. coli offers a crude guideline for the lethal level of glycerol ,, which appears consistent with the level we found for the total loss of motility and viability of C. crescentus (48%).
We also measured the osmolarity of 10% glycerol in comparison with that for 10% PEG 4000, 5% PEG 35000, and 5% PEO 400000. The results are listed in the Additional file 1: Table S1, confirming that glycerol produced much stronger osmotic pressure at comparable viscosity than that caused by introducing polymers into the cell medium. These additional measurements confirmed the mechanism detailed by earlier studies with glycerol, while also suggesting that adding inert polymers into the cell medium may be the better approach for the study of bacterial motility at elevated viscosity.
In conclusion, high percentage glycerol levels cause loss of motility and viability, likely attributable to hyperosmotic shock. In contrast, large PEG/PEO polymers appear to cause more moderate loss of motility by stalling the flagellar motor without killing the cells.
Polymer matrix attenuates the decrease of swimming speed
Here we switch gears to describe our findings on the influences of glycerol and polymer matrices on the swimming speed of the cells that remain motile. A close comparison of these two types of media led to insights into the physical process. Implications of the features we observed are briefly discussed.
In PEG 35000, we found a more gradual decrease in swimming speed with increasing viscosity (fit power −0.45; R = 0.96). In other words, the polymer network of PEG allowed for markedly higher speed as compared to an equally viscous solution of glycerol. This experimental result suggests that the swimming speed driven by a rotating flagellum is enhanced by the elasticity of the polymer matrix. In fact, more pronounced enhancements on swimming by a viscoelastic medium were previously shown on several other species of bacteria -. Note those earlier works used methylcellulose and polyvinylpyrrolidone (PVP), which are much larger in size than the polymers we used in this study. When we performed similar experiments with C. crescentus using methylcellulose, we observed a peak in swimming speed at ~0.05% (viscosity ~1.4 cP; data not shown). The latter result is consistent with previous observations of other species of bacteria -. The comparison here suggests that the difference in speed-viscosity behavior between our measured speeds in PEG 35000 (no peak) and those previously published by others using methylcellulose and PVP is likely due to the difference in matrix molecular size rather than species-specificity. The extremely large sizes of methylcellulose and PVP (on the order of a million daltons) appear to have caused enough enhancements on thrust, over-compensating the expected decrease in swimming speed due to increased drag. As a result, a peak in swimming speed has been predicted based on hydrodynamic calculations ,. Unfortunately, a rigorous comparison between the theoretical predictions and the experimental results is hampered by not knowing the actual size of the extremely high molecular weight polymers used in those experiments, and at the present time a lack of rheology data for those polymers at the low concentrations, which are necessary for bacteria to remain motile. In fact, our ongoing effort is designed to be conducive to more rigorous mechanical analysis using PEG and PEO, which cover a large range of available sizes.
Motor switching and stalling may be differentially regulated
Comparison in average intervals between switches of motor rotation
Wild-type in PYE
Wild-type in PEG 35000
Δpilin in PYE
Δpilin in PEG 35000
Number of Switching Intervals
Average Interval (s)
Standard Error (s)
Standard Deviation (s)
Our findings from the switching interval measurements strongly suggest that the switching and stalling of the flagellar motor are differentially regulated. The gradual loss of motility in either a viscous or viscoelastic medium does not depend on whether or not the motor switches (Figure 2); the motor switching frequency varies little with the addition of PEG 35000 up to 4%, which increases the apparent viscosity 5 fold. The loss of motility appears to be a sudden stall or jam, either due to injury caused by hyperosmotic shock at elevated levels of glycerol, or triggered by some mechanical failure under a high mechanical load or stress. Once the stalling occurs, the damage to the motor appears permanent, evidenced by the irrecoverable loss of motor function, and subsequent loss of the cell’s viability. We note that under the natural environment of relatively low fluid viscosity, the C. crescentus flagellar motor frequently switches its rotation direction with very short or even non-detectable stall time. In contrast, there are also species such as R. sphaeroides for which the motor can pause as part of its natural cycle of motion . We observed a few instances when a Caulobacter cell paused and then resumed motility.
This study was designed to assess and differentiate effects of solution viscosity and viscoelasticity on the swimming behavior of C. crescentus as a simple micro-swimmer with one helical flagellum. The results clearly show quantitative differences between a polymer matrix as a viscoelastic medium and a Newtonian fluid with elevated viscosity. In the Newtonian fluid, we found the swimming speed to scale inversely proportional to viscosity, as expected based on hydrodynamic theory for constant motor torque. In the test polymer network, we found the swimming speed to fall more gradually with viscosity, as v ~ η-0.45. This result suggests that the flagellated micro-swimmer produces a larger thrust at an increased polymer concentration, which partially offsets increased drag on the cell body. We were unable to quantitatively account for the measured results based on fluid mechanical calculations, due to lack of knowledge of detailed rheological properties of the PEG medium. Specifically, the apparent viscosity measurements performed in this study do not yield values of the storage and loss moduli as functions of the frequency of perturbance exerted by the rotating flagellum. Thus, additional characterization of the polymer matrix is required to better define the mechanical effects of the chosen media on the bacterial swimming speed.
By performing experiments using three selected strains, we were able to distinguish common behavior robustly accounted for by mechanical properties of the media with some notable exceptions, which offer insights into the functions of the flagella motor. The quantitative dependence of swimming speed on viscosity or concentration of polymers added is practically identical among all three strains. In both media, there is a significant difference in fraction of immobilized cells observed between the wild-type cells and the two strains lacking pili. Additionally, we saw nearly no effect of a viscoelastic medium on the switching frequency of the flagellar motor of C. crescentus. The results in this report call for further study on the control of the flagellar motor, as well as more detailed mechanical analysis. The latter requires high-speed imaging of the flagellum in order to determine its relative orientation to the cell body, as well as the hook that connects them. On the other hand, a more comprehensive characterization of the frequency dependent rheological properties of the polymer matrix is also required at the range most relevant to bacterial motility.
We conclude by suggesting that our study of bacterial motility in complex fluids using C. crescentus as a model system may spark interest in similar experiments on other flagellated bacteria in diverse environments, particularly those that find viscoelastic media their natural habitat.
Caulobacter crescentus strains CB15 wt, CB15 Δpilin (YB375) , and SB3860 were used. SB3860 is cheR138::Tn5 in YB375, ie., a mutant of CB15 Δpilin. It was kindly generated by Bert Ely, and was used in our previous studies ,. As described previously ,, the strains were grown in peptone yeast extract (PYE) medium  and synchronized with the plate release method , modified recently ,. The synchronization procedure yielded swarmer cells in fresh PYE that were within 5 minutes of division. The synchronized swarmer cells were immediately used in the experiments. Video recordings were terminated before the swarmer cells reached the age of 30 minutes, ensuring that properties observed were not complicated by the onset of differentiation towards the non-motile stage.
Viscometry and osmolarity measurements
We used polyethylene glycol of average sizes of 35,000 and 4000 daltons (PEG 35000 and PEG 4000, respectively; Sigma-Aldrich) and polyethylene oxide of 400,000 daltons (PEO 400000, Sigma-Aldrich). PEG 35000 has a radius of gyration of 8.6 nm ,, and the polymer becomes entangled in solutions at about 2% by weight or mass, estimated based on the Rouse model of polymer chains ,. The corresponding numbers for PEG 4000 are 2.9 nm and 6% and for PEO 400000 are 29 nm and 0.6%, respectively. Solutions of PEG 35000 or glycerol (Fisher Scientific) in PYE medium at room temperature were prepared to vary their viscosity up to 5–7 times to water. The PEG(PEO)/PYE and glycerol/PYE mixtures were made by first weighing a highly viscous stock solution such as 40% PEG 35000 (in PYE), 5% PEO 400000 (in PYE), or 100% glycerol, using an analytical balance, and then diluting it with the desired amount of PYE. The concentrations reported are by mass or weight, also commonly referred to as wt/wt percentage. Smaller sets of measurements were performed using methylcellulose (Sigma-Aldrich), which was prepared similarly by diluting from a 1% stock solution. The shear viscosities of the mixtures were measured using a size 50 pre-calibrated Cannon-Fenske Routine viscometer (CFRC 9721-B50 series, CANNON® Instrument Company, State College, PA). The measured viscosity values for PYE medium showed no significant difference from that of pure water. The measured values for glycerol-PYE mixtures were found in excellent agreement with those listed for glycerol-water mixtures in the Chemical Rubber Company (CRC) handbook with a temperature-related correction, as shown in the Additional file 1. Specifically, our viscosity measurements were performed at a room temperature of ~24°C, yielding values about 10% lower than those listed in CRC at 20°C.
We used the calibrated viscometer to measure the shear viscosity of the cell medium containing PEG or PEO. Solutions containing PEG 4000 over the 0-10% range yielded viscosities up to 3.0 cP. Solutions containing PEG 35000 over the 0-5% range yielded viscosities up to 6.5 cP. Solutions containing PEO 400000 over the 0-1% range yielded viscosities up to ~15 cP. We searched the literature and found these values to be consistent with values measured for PEG of several other molecular sizes, considering the known trend of a sharp increase with molecular weight at fixed mass concentration . Our measured values are tabulated and plotted in Additional file 1.
We measured the osmolarity of 10% glycerol, the PYE growth medium and selected PEG/PEO solutions, using a commercial osmometer based on freeze point depression detection (Osmette II, Precision Systems, Model 5005, Natick, MA).
Measurements of motility
An aliquot of synchronized cells in PYE was mixed with a viscous medium in a 1 mL centrifuge tube, by gently pipetting for 2–5 minutes, to uniformity. The final mixtures contained up to 10% of PEG 4000, 5% of PEG 35000, 0.5% of PEO 400000, or 48% of glycerol, all by mass. A 115–150 μL aliquot of bacteria-viscous agent mixture was then placed within a chamber formed by a 12.7 mm-diameter rubber O-ring, glued on top of a microscope slide using epoxy. The bacteria sample was made with sufficient depth (~1 mm) in order to collect data about swimming behavior far from the fluid boundary. A cover slip was placed on top of the O-ring filled with the sample to form a seal and allow for convenient observation using an upright microscope. Movies for counting motile versus non-motile cells were recorded between 2 and 5 minutes after mixing unless longer times were specified.
Images and videos were taken using a Nikon Eclipse E800 upright optical microscope with a mounted CoolSnap CCD camera (Photometrics, Tucson, AZ) and the software MetaMorph (Universal Imaging Corp., 2002). In order to capture C. crescentus during the swarmer stage of its life cycle, all observations were made on swarmer cells within 30 minutes in age.
To collect videos for motility detection, we used a long working distance 40x phase-contrast objective with a 0.05 s exposure time and a low capture rate of 6.7 frames/second. Under the 40x objective magnification, individual swarmer cells were discernable as opposed to cell aggregates or pre-divisional cells, which were both excluded from the counts. In contrast to swimming cells, which moved several microns per frame, non-motile cells displayed only diffusive motion and moved much less than 1 μm/s. Since the difference between them was over tenfold, the motile and non-motile cells were distinguished by eye. Percentages of immobilized cells were obtained by counting single swarmer cells that did not swim compared with those that were swimming in the recorded short videos.
Two methods were used to yield appropriate error bars. Method one applies to the percent motile data for strain 3860 in PEG 4000 and PEO 400000. Ten videos of ten frames each were taken within two minutes. Within each video, a few dozen cells were counted, out of which one value of percent motile was obtained. These ten values were averaged to yield the percentage motile along with an error bar. Method two applies to the percent motile data of all three strains in glycerol and PEG 35000. It extends beyond method one as similar measurements under these conditions were repeated two more rounds. The final percent motile data were shown as the average from the three rounds of measurements and the standard error from the three values. The errors are smaller by the second method since three times as many cells were counted under each condition of those measurements.
Measurements of swimming speed
For measurements of swimming speed, samples were prepared using the same rubber o-ring as for the motility assay. A sample was typically placed on the microscope stage for 5–7 minutes after mixing before recording to ensure the decay of fluid flow, which was the main source of error. A 20x objective was used with the dark-field setting of the microscope to take videos with 0.1s exposure time at 6.7 frames/second for 20–50 frames. The swimming speeds were obtained by analyzing the dark field videos using a custom-written MATLAB (Mathworks, Inc., Natick, MA) program. The program selects for swimming trajectories with positions discernable over ≥10 frames and without sharp changes of direction (threshold set at 1 radian/second) between frames in order to exclude measuring speed during motor switching events. Instantaneous speed was determined based on the spacing between consecutive cell body positions. The average speed over a short trajectory of each cell (during either forward or backward motion, as the difference in speed is negligible) was calculated for dozens or hundreds of cells under each condition. Due to the natural spread of swimming speed among individual cells, our data were plotted with standard deviation, which does not depend on how many cells were measured under each condition.
Measurements of cell viability
Following an established method , we used a fluorescent dye called DiBAC(4)3, (bis-[1,3-dibutylbarbiturate] trimethine oxonol) (Sigma-Aldrich, Inc.), which enters depolarized cells and exhibits enhanced fluorescence. Thus the DiBAC dye was chosen to label those C. crescentus cells that had lost their membrane integrity and were considered dead. A stock solution of 1 mg/mL DiBAC was dissolved in 1:1 ethanol:water mixture and diluted 1:1000 into synchronized swarmer cells in PYE (control), PYE containing 44% glycerol, or PYE containing 4% PEG 35000. Thin samples were prepared the same way as described below for the motor switching experiment. Each sample was imaged using both phase contrast and fluorescence microscopy. A long exposure time (0.5 s) was used so that motile cells appeared as faint streaks under phase contrast. The number of non-motile cells appearing in each image was counted and the fraction of non-motile cells that were dead (i.e. appeared under both phase contrast and fluorescence) was calculated.
Measurements of motor switching
For measurements of motor switching, thinner samples were found to yield better images as the cells were prevented from moving out of the focal plane. A 5 μL sample was pressed between a microscope slide and a coverslip, sealed with a layer of vacuum grease around the edges. The sample thickness of such preparations was on the order of 10 μm.
Videos were taken using a Photron FASTCAM-PCI R2 high-speed camera mounted to a Nikon Eclipse TE2000-U inverted optical microscope. Videos were recorded using a 20x phase-contrast objective with a frame rate of 125 frames/second. The time interval between motor switches was measured by visually identifying the frames at which a particular cell underwent a reversal in its direction of travel. The time interval between two consecutive switching events was then calculated based on the known video frame rate. The high frame rate ensured that the measurement error was within 10 milliseconds, far shorter than the intervals between motor switches, which typically occur on the order of a second.
This work was supported by National Science Foundation Awards PHY-1058375 and NSF CBET 1438033. JXT thanks Professor Howard Berg and the Rowland Institute of Harvard University for hosting his sabbatical stay, partially supported by NIH Grant AI100902. He also acknowledges the hospitality of the Aspen Center for Physics, which is supported by the National Science Foundation Grant No. PHY-1066293.
We thank Professors Y. Brun and B. Ely for providing us the bacterial strains used in the study. We acknowledge Professors Thomas Powers and James Valles Jr. for helpful discussions. Linda Turner-Stern and Pushkar Lele are acknowledged for their critical reading of the manuscript.
- Berg HC: The rotary motor of bacterial flagella. Annu Rev Biochem. 2003, 72: 19-54. 10.1146/annurev.biochem.72.121801.161737.View ArticlePubMedGoogle Scholar
- Lele PP, Hosu BG, Berg HC: Dynamics of mechanosensing in the bacterial flagellar motor. Proc Natl Acad Sci U S A. 2013, 110 (29): 11839-11844. 10.1073/pnas.1305885110.PubMed CentralView ArticlePubMedGoogle Scholar
- Fahrner KA, Ryu WS, Berg HC: Biomechanics: bacterial flagellar switching under load. Nature. 2003, 423 (6943): 938-10.1038/423938a.View ArticlePubMedGoogle Scholar
- Son K, Guasto J, Stocker R: Bacteria can exploit a flagellar buckling instability to change direction. Nat Commun. 2013, 9: 494-498.Google Scholar
- Xie L, Altindal T, Chattopadhyay S, Wu XL: Bacterial flagellum as a propeller and as a rudder for efficient chemotaxis. Proc Natl Acad Sci. 2011, 108 (6): 2246-2251. 10.1073/pnas.1011953108.PubMed CentralView ArticlePubMedGoogle Scholar
- Greenberg EP, Canale-Parola E: Motility of flagellated bacteria in viscous environments. J Bacteriol. 1977, 132 (1): 356-358.PubMed CentralPubMedGoogle Scholar
- Schneider WR, Doetsch RN: Effect of viscosity on bacterial motility. J Bacteriol. 1974, 117 (2): 696-701.PubMed CentralPubMedGoogle Scholar
- Shoesmith JG: The measurement of bacterial motility. J Gen Microbiol. 1960, 22 (2): 528-535. 10.1099/00221287-22-2-528.View ArticleGoogle Scholar
- Berg HC, Turner L: Movement of microorganisms in viscous environments. Nature. 1979, 278 (5702): 349-351. 10.1038/278349a0.View ArticlePubMedGoogle Scholar
- Magariyama Y, Kudo S: A mathematical explanation of an increase in bacterial swimming speed with viscosity in linear-polymer solutions. Biophys J. 2002, 83 (2): 733-739. 10.1016/S0006-3495(02)75204-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Fu H, Wolgemuth CW, Powers TR: Swimming of filaments in nonlinearly viscoelastic fluids. Phys Fluids. 2009, 21: 033102-10.1063/1.3086320.View ArticleGoogle Scholar
- Liu B, Powers TR, Breuer KS: Force-free swimming of a model helical flagellum in viscoelastic fluids. Proc Natl Acad Sci U S A. 2011, 108 (49): 19516-19520. 10.1073/pnas.1113082108.PubMed CentralView ArticlePubMedGoogle Scholar
- Atsumi T, Maekawa Y, Yamada T, Kawagishi I, Imae Y, Homma M: Effect of viscosity on swimming by the lateral and polar flagella of vibrio alginolyticus. J Bacteriol. 1996, 178 (16): 5024-5026.PubMed CentralPubMedGoogle Scholar
- Chen X, Berg HC: Torque-speed relationship of the flagellar rotary motor of escherichia coli. Biophys J. 2000, 78 (2): 1036-1041. 10.1016/S0006-3495(00)76662-8.PubMed CentralView ArticlePubMedGoogle Scholar
- Li G, Tang JX: Low flagellar motor torque and high swimming efficiency of caulobacter crescentus swarmer cells. Biophys J. 2006, 91 (7): 2726-2734. 10.1529/biophysj.106.080697.PubMed CentralView ArticlePubMedGoogle Scholar
- Ferry J: Viscoelastic Properties of Polymers. 1980, John Wiley, New York, 3Google Scholar
- Larson RG: The Structure and Rheology of Complex Fluids. 1999, Oxford University Press, New YorkGoogle Scholar
- Gode-Potratz CJ, Kustusch RJ, Breheny PJ, Weiss DS, McCarter LL: Surface sensing in vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence. Mol Microbiol. 2011, 79 (1): 240-263. 10.1111/j.1365-2958.2010.07445.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson JK, Smith TG, Hoover TR: Sense and sensibility: flagellum-mediated gene regulation. Trends Microbiol. 2010, 18 (1): 30-37. 10.1016/j.tim.2009.11.001.PubMed CentralView ArticlePubMedGoogle Scholar
- Kearns DB: A field guide to bacterial swarming motility. Nat Rev Microbiol. 2010, 8 (9): 634-644. 10.1038/nrmicro2405.PubMed CentralView ArticlePubMedGoogle Scholar
- Rather PN: Swarmer cell differentiation in proteus mirabilis. Environ Microbiol. 2005, 7 (8): 1065-1073. 10.1111/j.1462-2920.2005.00806.x.View ArticlePubMedGoogle Scholar
- Li G, Brown PJ, Tang JX, Xu J, Quardokus EM, Fuqua C, Brun YV: Surface contact stimulates the just-in-time deployment of bacterial adhesins. Mol Microbiol. 2012, 83 (1): 41-51. 10.1111/j.1365-2958.2011.07909.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Poindexter JS: Biological properties and classification of the caulobacter crescentus group. Bacteriol Rev. 1964, 28: 231-295.PubMed CentralPubMedGoogle Scholar
- Boutte, CC, Crosson, S: Bacterial lifestyle shapes stringent response activation.Trends Microbiol 2013, 21(4):174–180.,Google Scholar
- Poindexter JS: The caulobacters: ubiquitous unusual bacteria. Microbiol Rev. 1981, 45 (1): 123-179.PubMed CentralPubMedGoogle Scholar
- Lawler ML, Brun YV: Advantages and mechanisms of polarity and cell shape determination in caulobacter crescentus. Curr Opin Microbiol. 2007, 10 (6): 630-637. 10.1016/j.mib.2007.09.007.PubMed CentralView ArticlePubMedGoogle Scholar
- Wagner JK, Setayeshgar S, Sharon LA, Reilly JP, Brun YV: A nutrient uptake role for bacterial cell envelope extensions. Proc Natl Acad Sci U S A. 2006, 103 (31): 11772-11777. 10.1073/pnas.0602047103.PubMed CentralView ArticlePubMedGoogle Scholar
- Li G, Smith CS, Brun YV, Tang JX: The elastic properties of the caulobacter crescentus adhesive holdfast are dependent on oligomers of N-Acetylglucosamine. J Bacteriol. 2005, 187 (1): 257-265. 10.1128/JB.187.1.257-265.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Merker RI, Smit J: Characterization of the adhesive holdfast of marine and freshwater caulobacters. Appl Environ Microbiol. 1988, 54 (8): 2078-2085.PubMed CentralPubMedGoogle Scholar
- Bodenmiller D, Toh E, Brun YV: Development of surface adhesion in caulobacter crescentus. J Bacteriol. 2004, 186 (5): 1438-1447. 10.1128/JB.186.5.1438-1447.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Entcheva-Dimitrov P, Spormann AM: Dynamics and control of biofilms of the oligotrophic bacterium caulobacter crescentus. J Bacteriol. 2004, 186 (24): 8254-8266. 10.1128/JB.186.24.8254-8266.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Skerker JM, Shapiro L: Identification and cell cycle control of a novel pilus system in caulobacter crescentus. EMBO J. 2000, 19 (13): 3223-3234. 10.1093/emboj/19.13.3223.PubMed CentralView ArticlePubMedGoogle Scholar
- Li G, Tam L-K, Tang JX: Amplified effect of brownian motion in bacterial near-surface swimming. Proc Natl Acad Sci. 2008, 105 (47): 18355-18359. 10.1073/pnas.0807305105.PubMed CentralView ArticlePubMedGoogle Scholar
- Li GL, Bensson J, Nisimova L, Munger D, Mahautmr P, Tang JX, Maxey MR, Brun YV: Accumulation of swimming bacteria near a solid surface. Phys Rev E. 2011, 84 (4): 041932-10.1103/PhysRevE.84.041932.View ArticleGoogle Scholar
- Li, GL and JX Tang: Accumulation of microswimmers near a surface mediated by collision and rotational brownian motion, Phys Rev Lett 2009, 103(7):078101.,Google Scholar
- Beney L, Mille Y, Gervais P: Death of escherichia coli during rapid and severe dehydration is related to lipid phase transition. Appl Microbiol Biotechnol. 2004, 65 (4): 457-464. 10.1007/s00253-004-1574-x.View ArticlePubMedGoogle Scholar
- Howard DH: The preservation of bacteria by freezing in glycerol broth. J Bacteriol. 1956, 71 (5): 625-PubMed CentralPubMedGoogle Scholar
- Morris GJ, Goodrich M, Acton E, Fonseca F: The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation. Cryobiology. 2006, 52 (3): 323-334. 10.1016/j.cryobiol.2006.01.003.View ArticlePubMedGoogle Scholar
- Liu B, Gulino M, Morse M, Tang JX, Powers TR, Breuer KS: Helical motion of the cell body enhances caulobacter crescentus motility. Proc Natl Acad Sci U S A. 2014, 111 (31): 11252-11256. 10.1073/pnas.1407636111.PubMed CentralView ArticlePubMedGoogle Scholar
- Yuan J, Fahrner KA, Turner L, Berg HC: Asymmetry in the clockwise and counterclockwise rotation of the bacterial flagellar motor. Proc Natl Acad Sci U S A. 2010, 107 (29): 12846-12849. 10.1073/pnas.1007333107.PubMed CentralView ArticlePubMedGoogle Scholar
- Braun TF, Al-Mawsawi LQ, Kojima S, Blair DF: Arrangement of core membrane segments in the mota/motb proton-channel complex of escherichia coli. Biochemistry. 2004, 43 (1): 35-45. 10.1021/bi035406d.View ArticlePubMedGoogle Scholar
- Brown MT, Delalez NJ, Armitage JP: Protein dynamics and mechanisms controlling the rotational behaviour of the bacterial flagellar motor. Curr Opin Microbiol. 2011, 14 (6): 734-740. 10.1016/j.mib.2011.09.009.View ArticlePubMedGoogle Scholar
- Lele PP, Branch RW, Nathan VS, Berg HC: Mechanism for adaptive remodeling of the bacterial flagellar switch. Proc Natl Acad Sci U S A. 2012, 109 (49): 20018-20022. 10.1073/pnas.1212327109.PubMed CentralView ArticlePubMedGoogle Scholar
- Yuan J, Branch RW, Hosu BG, Berg HC: Adaptation at the output of the chemotaxis signalling pathway. Nature. 2012, 484 (7393): 233-236. 10.1038/nature10964.PubMed CentralView ArticlePubMedGoogle Scholar
- Yuan J, Berg HC: Ultrasensitivity of an adaptive bacterial motor. J Mol Biol. 2013, 425 (10): 1760-1764. 10.1016/j.jmb.2013.02.016.View ArticlePubMedGoogle Scholar
- Teran J, Fauci L, Shelley M: Viscoelastic fluid response can increase the speed and efficiency of a free swimmer. Phys Rev Lett. 2010, 104: 038101-10.1103/PhysRevLett.104.038101.View ArticlePubMedGoogle Scholar
- Armitage JP, Schmitt R: Bacterial chemotaxis: rhodobacter sphaeroides and sinorhizobium meliloti--variations on a theme?. Microbiology. 1997, 143 (Pt 12): 3671-3682. 10.1099/00221287-143-12-3671.View ArticlePubMedGoogle Scholar
- Morse M, Huang A, Li G, Maxey MR, Tang JX: Molecular adsorption steers bacterial swimming at the air/water interface. Biophys J. 2013, 105: 21-28. 10.1016/j.bpj.2013.05.026.PubMed CentralView ArticlePubMedGoogle Scholar
- Degnen ST, Newton A: Chromosome replication during development in caulobacter crescentus. J Mol Biol. 1972, 64 (3): 671-680. 10.1016/0022-2836(72)90090-3.View ArticlePubMedGoogle Scholar
- Holyst R, Bielejewska A, Szymanski J, Wilk A, Patkowski A, Gapinski J, Zywocinski A, Kalwarczyk T, Kalwarczyk E, Tabaka M, Ziebacz N, Wieczorek SA: Scaling form of viscosity at all length-scales in poly(Ethylene Glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis. Phys Chem Chem Phys. 2009, 11 (40): 9025-9032. 10.1039/b908386c.View ArticlePubMedGoogle Scholar
- Doi M, Edwards SF: The Theory of Polymer Dynamics. 1986, Clarendon, OxfordGoogle Scholar
- Watanabe H: Viscoelasticity and dynamics of entangled polymers. Prog Polym Sci. 1999, 24: 1253-1403. 10.1016/S0079-6700(99)00029-5.View ArticleGoogle Scholar
- Smit J, Sherwood CS, Turner RF: Characterization of high density monolayers of the biofilm bacterium caulobacter crescentus: evaluating prospects for developing immobilized cell bioreactors. Can J Microbiol. 2000, 46 (4): 339-349. 10.1139/w99-145.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.