Global transcriptional responses to the bacteriocin colicin M in Escherichia coli
© Kamenšek and Žgur-Bertok; licensee BioMed Central Ltd. 2013
Received: 31 August 2012
Accepted: 18 February 2013
Published: 19 February 2013
Bacteriocins are protein antimicrobial agents that are produced by all prokaryotic lineages. Escherichia coli strains frequently produce the bacteriocins known as colicins. One of the most prevalent colicins, colicin M, can kill susceptible cells by hydrolyzing the peptidoglycan lipid II intermediate, which arrests peptidoglycan polymerization steps and provokes cell lysis. Due to the alarming rise in antibiotic resistance and the lack of novel antimicrobial agents, colicin M has recently received renewed attention as a promising antimicrobial candidate. Here the effects of subinhibitory concentrations of colicin M on whole genome transcription in E. coli were investigated, to gain insight into its ecological role and for purposes related to antimicrobial therapy.
Transcriptome analysis revealed that exposure to subinhibitory concentrations of colicin M altered expression of genes involved in envelope, osmotic and other stresses, including genes of the CreBC two-component system, exopolysaccharide production and cell motility. Nonetheless, there was no induction of biofilm formation or genes involved in mutagenesis.
At subinhibitory concentrations colicin M induces an adaptive response primarily to protect the bacterial cells against envelope stress provoked by peptidoglycan damage. Among the first induced were genes of the CreBC two-component system known to promote increased resistance against colicins M and E2, providing novel insight into the ecology of colicin M production in natural environments. While an adaptive response was induced nevertheless, colicin M application did not increase biofilm formation, nor induce SOS genes, adverse effects that can be provoked by a number of traditional antibiotics, providing support for colicin M as a promising antimicrobial agent.
In their natural environments, bacteria are frequently exposed to various stresses, including antimicrobials. It has been generally assumed that the role of antibiotics in nonclinical environments is the inhibition of competitors. Nevertheless, antibiotic concentrations in natural habitats can be variable, with high concentrations only in the vicinity of the producer. Recent studies have shown that antibiotics can act in a concentration-dependent manner that exhibits dual ecological roles: (i) at high concentrations they can destroy microorganisms; while (ii) at low concentrations they can modulate bacterial gene expression to promote ecological adaptation[1, 2].
Bacteriocins are ribosomally synthesized antimicrobial agents that are produced by all prokaryotic lineages and they are generally active against closely related species. Among the best characterized bacteriocins are those produced by Escherichia coli, which are known as colicins. The majority of colicins act by membrane permeabilization, followed by nuclease activity, while one colicin, colicin M, inhibits peptidoglycan synthesis.
Uptake of colicin M proceeds by binding to the FhuA outer membrane receptor followed by energy-dependent translocation into the periplasm through the TonB system (TonB, ExbB and ExbD) and the proton motive force of the inner membrane. Colicin M is a phosphotase that cleaves the undecaprenyl-phosphate-linked peptidoglycan precursor, lipid II producing free undecaprenol and 1-pyrophospho-Mur-GlCNAc-pentapeptide. In the periplasm, hydrolysis of peptidoglycan lipid precursors results in arrest of polymerization steps and cell lysis. Operons that encode colicin M and B are tightly linked on large conjugative plasmids[5, 6], and these are among the most abundant colicins produced by E. coli strains.
A number of studies have been aimed at defining the function of colicins in microbial communities. They might serve to enable invasion or defense of an ecological niche. They have been shown to mediate population and community level interactions, promoting microbial diversity within E. coli populations in the mammalian colon. To obtain more insight into the ecological roles of one of the most prevalent colicins, the effects of subinhibitory concentrations of colicin M on genome wide transcription in E. coli was studied.
Antibiotic resistance currently represents one of the greatest worldwide threats to human health therefore, novel antibiotics are urgently needed. Antibiotic resistance among the Enterobacteriaceae represents a particular threat[10, 11]. As colicin M promotes the irreversible hydrolysis of lipid II, a peptidoglycan lipid intermediate that is common to all bacteria, it is also a promising candidate for development of a novel antimicrobial agent. Analysis of the gene expression profile was thus also undertaken, to acquire insight into adaptive responses to colicin M that might be detrimental during antimicrobial therapy.
Results and discussion
Transcriptome analysis of E. coli MG1655 exposed to subinhibitory concentrations of colicin M
Genes with modulated expression after exposure to colicin M over time, 30 and 60 min
Gene accession No.
30 min log2ratio
60 min log2ratio
Envelope stress regulators/systems
DNA-binding transcriptional activator, co-regulator with RcsB
inhibitor of the cpx response; periplasmic adaptor protein
regulatory protein for phage-shock-protein operon
DNA-binding transcriptional regulator of psp operon
DNA-binding transcriptional activator
peripheral inner membrane phage-shock protein
phage shock protein G
Colanic acid biosynthetic process
lipoprotein required for capsular polysaccharide translocation through the outer membrane
predicted glycosyl transferase
predicted acyl transferase
predicted glycosyl transferase
predicted colanic acid polymerase
predicted glycosyl transferase
predicted acyl transferase
GDP-D-mannose dehydratase, NAD(P)-binding
bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/GDP-4-dehydro-6-L-deoxygalactose reductase
GDP-mannose mannosyl hydrolase
predicted glycosyl transferase
predicted UDP-glucose lipid carrier transferase
predicted colanic acid exporter
Colanic acid biosynthesis protein
predicted glycosyl transferase
colanic acid biosynthesis protein
Extracellular polysaccharide distinct from colanic acid
anhydro-N-acetylmuramic acid kinase
fused glycosyl transferase and transpeptidase
L,D-transpeptidase linking Lpp to murein
Osmotic stress response
osmotically inducible, stress-inducible membrane protein
phosphoglycerol transferases I and II
membrane protein required for succinylation of osmoregulated periplasmic glucans (OPGs)
inhibitor of vertebrate C-lysozyme
inhibitor of C-lysozyme, membrane-bound; predicted lipoprotein
predicted mechanosensitive channel
Other stress responses
DNA-binding transcriptional repressor
heat shock chaperone
heat shock chaperone
heat-inducible lipoprotein involved in novobiocin resistance
predicted intracellular protease
RpoS stabilizer during Mg starvation, anti-RssB factor
inner membrane protein
inner membrane protein, creBC regulon
predicted oxidoreductase with FAD/NAD(P)-binding domain
conserved protein, UPF0167 family
envelope stress induced periplasmic protein
heat shock protein binding to Era protein; predicted peptidase
biofilm-dependent modulation protein
diguanylate cyclase, required for pgaD induction
RpoS antagonist; putative regulator of FliA activity
flagellar basal-body component
flagellar motor switching and energizing component
export chaperone for FlgK and FlgL
assembly protein for flagellar basal-body periplasmic P ring
flagellar component of cell-proximal portion of basal-body rod
anti-sigma factor for FliA (sigma 28)
RNA polymerase, sigma 28 (sigma F) factor
flagellar hook assembly protein
flagellar hook protein
flagellar component of cell-proximal portion of basal-body rod
flagellar component of cell-proximal portion of basal-body rod
DNA-binding transcriptional dual regulator with FlhD
DNA-binding transcriptional dual regulator with FlhC
Amino acid transport/acid resistance
glutamine transporter subunit
glutamate decarboxylase B, PLP-dependent
glutamine transporter subunit
fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein
glutamate decarboxylase A, PLP-dependent
DNA-binding transcriptional activator
outer membrane lipoprotein
acid-resistance membrane protein
conserved protein, UPF0757 family
predicted inner membrane protein
conserved protein, UPF0337 family
phosphatidylglycerol kinase, metal-dependent
inner membrane protein, DUF2755 family
Colicin M treatment affects signal transduction pathways
The bacterial envelope protects the bacterial cell from external stress and performs essential functions such as, transport of nutrients and waste, as well as respiration and adhesion. In Gram-negative bacteria the outer membrane acts as a permeability barrier, with porins for transport and protection against phagocytosis while processes central for energy generation and nutrient transport occur in the inner membrane. In the periplasmic space peptidoglycan ensures the structural integrity of the cell by preventing osmolysis. To sense and rapidly respond to environmental signals, bacteria primarily use two-component signal transduction systems, composed of an inner membrane histidine kinase and a cytoplasmic response regulator.
Peptidoglycan is the major structural component of the bacterial cell wall. It provides the bacterial cell structural strength and protects the osmotically sensitive membrane. As expected, by targeting peptidoglycan synthesis, colicin M induced an envelope stress response. In E. coli envelope homeostasis is monitored by several stress responses; namely, Rcs, Cpx, Psp, σE, Bae and vesicle release responses[18–20]. Our microarray analysis revealed that the Rcs regulon has a major role in the response to envelope stress induced by colicin M. RcsC and RcsD are inner-membrane-bound proteins, with RcsC as the sensor kinase that autophosphorylates upon sensing the appropriate signal, while RcsD transfers the phosphoryl group to the transcriptional regulator RcsB. Certain promoters require the RcsA protein to act as an auxiliary regulatory protein, apparently exerting its effects by forming a heterodimer with RcsB. The Rcs system controls the production of exopolysaccharides, biofilm formation[23, 24], cell motility, and chemotaxis. We observed induction of rcsA and a number of other Rcs-regulated genes: the exopolysaccharide operons wca for colanic acid synthesis, and yjbEFGH, as well as genes osmB, ymgG and ymgD, ivy, yfbR, ugd, yfdC, yjbJ, galU, yaaX, yggG, yegS, spy, rprA, bdm and yaiY (Table 1). Recently, perturbations to peptidoglycan by several ß-lactam antibiotics were shown to elicit shared as well as unique responses with all activating the Rcs system indicating that, the Rcs pathway elicits a global response to peptidoglycan stress.
Colicin M treatment also induced the expression of cpxP, which encodes the periplasmic inhibitor of the Cpx envelope stress response. The Cpx system appears to sense misfolded proteins that are synthesized for the periplasm, and it is controlled by the sensor kinase CpxA, the response regulator CpxR, and the periplasmic inhibitor CpxP. CpxP has been assumed to fine tune Cpx activation during envelope stress, by preventing its incorrect activation and enabling its rapid shut-down following envelope stress relief.
Treatment with colicin M also up-regulated a third cell envelope stress system, the psp genes that encode the membrane-bound phage shock proteins: PspA, PspB, PspC, PspD and PspG. The psp regulon consists of the psp operon with genes pspA, pspB, pspC, pspD and pspE, as well as genes pspF and pspG. Proteins PspB, PspC and PspD are located in the inner membrane, while PspA is on the cytoplasmic side of the inner membrane. In the absence of stress, PspA binds to protein PspF, thus inhibiting transcription of the psp operon. The psp genes are induced by filamentous phage infection and other stresses that impair cell membrane function[29, 30]. This system is responsible for repair of inner membrane damage and maintenance of the proton motive force across the inner membrane[31, 32]. Peptidoglycan damage provoked by colicin M exposes the sensitive inner membrane to osmotic damage requiring activation of membrane repair mechanisms.
Colicin M induces expression of exopolysaccharide genes
Among the most strongly up-regulated genes, were those of the wca operon, which encodes the production of the exopolysaccharide, colanic acid. The highly viscous colanic acid is secreted into the extracellular environment to protect cells from osmotic stress such as provoked by cell envelope perturbations, including peptidoglycan damage or dessication. In addition, colanic acid is involved in the later stages of biofilm formation; namely, the maturation and development of complex three-dimensional biofilm structures. The wca operon is comprised of 19 genes that are involved in colanic acid synthesis from the nucleoside diphosphate sugars: GDP-L-fucose, UDP-d-glucose, UDP-d-galactose and UDP-D-glucuronate. Colicin M treatment induced the expression of all 19 of the wca genes.
Exposure to colicin M also up-regulated the D-galactose transporter galP, as well as galU, which encodes the glucose-1-phosphate uridylyltransferase that is needed for UDP-glucose, an intermediate involved in the synthesis of colanic acid, trehalose, lipopolysaccharide and membrane-derived oligosaccharides.
Furthermore, our studies revealed strong induction of the yjbEFGH operon that is involved in the production of another, as-yet-unidentified, exopolysaccharide. Recent studies have shown that the yjbEFGH operon is also induced by osmotic stress, and that the wca and yjbEFGH operons are negatively regulated by the general stress response sigma factor RpoS (σ38). Both the wca and the yjbEFGH operons are induced by the activated Rcs pathway to protect the bacterial cell from osmolysis.
Colicin M induced additional osmotic and other stress responses
By inhibiting peptidoglycan synthesis, colicin M weakens membrane protection, provoking osmotic stress. Interestingly, genes creD, cbrA, cbrB and cbrC of the CreB/CreC regulon were strongly induced already 30 min after exposure to colicin M. The Cre system was previously found to be involved in the switch from aerobic to anaerobic conditions. CreC is the sensor that also senses changes in the growth medium and/or metabolite pool levels, while CreB is a transcriptional regulator. The two-component CreBC system positively controls transcription of cbrA. Recently, the CbrA protein was shown to protect against colicin M and osmotic shock, implying a function of CbrA in outer membrane structure. Thus, it was proposed that in the natural environment with a limited supply of nutrients, CbrA protects sensitive cells from colicin M synthesized by competitors. Furthermore, CbrC, which we also found to be induced by colicin M treatment, has been shown to protect against colicin E2 and also seems to be involved in alteration of outer membrane structure. Our results indicate that subinhibitory concentrations of colicin M could induce protection against colicins. Thus, in the natural environment, both colicin synthesis and the CreBC system are induced upon nutrient limitation[42, 43]. Colicin produced in microbial communities by colicinogenic bacteria could in colicin sensitive community members induce protective responses. Moreover, activation of the CreBC two component regulator system was recently shown to play a major role in the ß-lactam resistance response indicating that, subinhibitory concentrations of colicin M might elicit broader antimicrobial protection.
It can also be noted that more than 100 of the open reading frames up-regulated by colicin M treatment are classified as poorly characterized or with predicted functions. Among these, many are predicted membrane proteins and lipoproteins indicating that, to protect cells against peptidoglycan damage provoked by colicin M, an adaptive response to strengthen/stabilize the osmosensitive membrane is induced.
To resist the effects of colicin M treatment, other genes involved in the response to hyperosmotic stress were up-regulated; namely, osmB and osmC as well as two inhibitors of C-lysozyme, ivy and a membrane bound and predicted lipoprotein mliC, were also induced by the Rcs system. Antibiotic-mediated peptidoglycan stress has also been shown to trigger expression of both of these genes.
Colicin M also induced other stress response genes, including ydeI, which is involved in hydrogen peroxide stress, as well as the ibpA and ibpB heat shock genes, which encode chaperones that can cooperate to prevent irreversible aggregation of proteins.
Colicin M induces biofilm associated genes
In natural environments, bacteria often form biofilms, microbial communities in which bacteria adhere to an abiotic or biotic surface via surface charges as well as production of pili, fimbriae and exopolysaccharides. Microbial cells in biofilms show distinct properties, particularly resistance to antibiotics, disinfectants, shear stress and the immune system. Biofilm formation proceeds in several tightly regulated steps: initial attachment, three-dimensional development by microcolony formation, biofilm maturation and the final step dispersal or cellular detachment to colonize other surfaces. Initially, flagella promote motility toward a surface; subsequently, flagella are lost and adhesive organelles such as curli fimbria enable attachment; and finally, colanic acid production promotes maturation into the three dimensional biofilm structure[49, 50].
Colicin M treatment upregulated several genes involved in biofilm production. Thus, in addition to up-regulation of colanic acid, colicin M treatment up-regulated rprA, which encodes a small regulatory RNA affecting target mRNA translation. Expression of rprA has been shown to be activated by the Rsc system. RprA has been shown to repress csgD. This latter encodes the master transcriptional regulator that activates curli fimbriae and cellulose synthesis, both of which are involved in the initial stage of biofilm formation. It has been postulated that by interfering with csgD mRNA translation, RprA might prevent the undesired co-expression of curli/cellulose and colanic acid. In accordance, as we found upregulation of the colanic acid operon genes we also determined upregulation of the omrA and omrB genes, which encode two redundant small/antisense RNAs that have recently been shown to inhibit CsgD translation.
Colicin M exposure up-regulated another biofilm-associated gene, bdm, which encodes the biofilm-dependent modulation protein. Bdm expression is positively regulated by RcsB in response to osmotic shock, and the Bdm protein has been recently shown to enhance biofilm formation.
The exposure to colicin M also upregulated ydeH, which codes for a diguanylate cyclase that can synthesize the second messenger bis-(3′-5′) cyclic di-guanosine monophosphate (c-di-GMP)[55–57]. ydeH is positively regulated by CpxR, and has been shown to inhibit motility as well as to promote adhesin and biofilm formation. In E. coli, c-di-GMP controls the synthesis of two exopolysaccharides: cellulose and poly-GlcNaC (PGA), a virulence factor of uropathogenic E. coli. Our study thus showed that colicin M induced an envelope stress response which could provoke switching from a planktonic to a sessile lifestyle. Nevertheless, in crystal violet assays no induction of biofilm formation was observed (data not shown).
Colicin M treatment downregulates flagellar biosynthesis genes
Not unexpectedly, among the down-regulated genes, there were in particular the genes involved in flagellar motility and in glutamine biosynthetic processes. In E. coli, flagellar expression and motility is controlled by the FlhDC complex that comprises >60 genes. Flagellar synthesis and function are processes that demand high energy consumption, and therefore, expression of the flagellar genes is tightly regulated. In contrast to exopolysaccharide production, expression of the flhDC operon has been shown to be down-regulated by numerous environmental signals, such as high temperature, high osmolarity (concentrations of salts, in the presence of carbohydrates or low-molecular alcohols) and extreme pH[60, 61].
Both the exopolysaccharide synthesis operons, wca and yjbEFGH, and the flagellar flhDC operon genes are controlled by the Rcs phosphorelay system. However, while the activated Rcs phosphorelay system induces exopolysaccharide synthesis, it down-regulates the flhDC operon due to repression by the RcsB cofactor RcsA.
Validation of microarray results by real-time PCR
Colicin M treatment does not promote significantly increased exopolysaccharide production
As the microarray data showed that the colanic acid operon genes were among the most strongly induced, a functional assay was performed to address whether the amount of colanic acid was changed accordingly. Production of colanic acid was quantified following exposure of E. coli to colicin M. Colanic acid was extracted from bacterial cultures treated with subinhibitory concentrations of colicin M for 60 min, 90 min and 120 min, as well as from an untreated control. While at the mRNA level there was significant induction of the wca operon genes, only a slight, 1.3-fold, increase in the production of colanic acid was seen at all sampling times. As an additional control, colanic acid was quantified from a culture overexpressing the wca operon encoded by a multicopy plasmid, pATC400. A 6-fold increase in colanic acid production was seen in comparison with an isogenic strain that did not overexpress the wca operon genes.
Treatment of E. coli with colicin M promotes the hydrolysis of the peptidoglycan lipid precursors, which results in the arrest of the polymerization steps and exposes the bacterial cells to envelope stress, which activates the Rcs and Cpx phosphorelay systems. Subsequently, cell motility is down-regulated, with induction of the expression of the exopolysaccharide wca and the yjbEFGH operon genes. Colicin M promoted hydrolysis of lipid II which prevents recycling of the lipid carrier for peptidoglycan synthesis and also limits its availability for exopolysaccharide biosynthesis, including colanic acid.
Following an initial growth stagnation (Figure 1 and also see Additional file1: Figure S1), regrowth of cultures treated with these subinhibitory concentrations of colicin M indicate an adaptive response to the stress through the activation of the envelope and other stress responses. While neither an increase in colanic acid or biofilm production was detected, in the natural environment, activation of the various above-described stress responses might allow rapid adaptation, including mature biofilm formation, in the event of a sudden decrease/absence in antimicrobial concentrations.
Genome-wide transcriptional analysis and other antimicrobials
A number of studies have shown that traditional antibiotics affect bacterial gene expression and physiology[1, 2, 63, 64]. Thus, some β-lactam antibiotics that can also inhibit peptidoglycan synthesis have been shown to induce the production of colanic acid in E. coli, which indicates that these might exacerbate biofilm formation. Investigation of the E. coli transcription profile in response to bactericidal concentrations of ampicillin also showed induction of the colanic acid biosynthetic pathway, as well as rcsA, the transcriptional activator of colanic acid synthesis and other stress responses. However, the authors did not detect induction of the additional exopolysaccharide operon yjbEFGH, distinct from colanic acid. In Staphylococcus aureus, subinhibitory concentrations of β-lactams have been shown to up-regulate some virulence genes. Moreover, the aminoglycoside tobramycin has been shown to induce biofilm formation in E. coli and in Pseudomonas aeruginosa, due to alterations in the levels of c-di-GMP. Biofilm formation was also induced following exposure of P. aeruginosa to subinhibitory concentrations of tetracycline and norfloxacin.
Further to this, a number of studies have investigated the effects of antibiotics on the expression of the SOS regulon genes. Thus, the β-lactam antibiotic, ceftazidime, which is an inhibitor of a protein involved in cell wall biosynthesis, PBP3, has been shown to induce transcription of the dinB gene, which encodes the error-prone DNA polymerase Pol IV. Subsequently, subinhibitory concentrations of ampicillin, norfloxacin and kanamycin were shown to induce mutagenesis due to antibiotic-mediated increases in reactive oxygen species, which results in SOS-induced mutagenesis that might lead to multidrug resistance. An additional study showed that a number of antibiotics can promote an increase in mutation frequency; namely, ampicillin, ceftazidime, imipenem, ciprofloxacin, trimethoprim, sulfamethoxazole and tetracycline. With the exception of imipenem, fosfomycin and tetracycline, the antibiotics tested were shown to induce recA expression, while inactivation of recA abolished the mutagenic effects.
In the present study, subinhibitory concentrations of colicin M did not induce the expression of dinB or recA. To further confirm that colicin M does not induce the SOS response, induction of the sulA gene following colicin M treatment was investigated. SOS-regulated SulA inhibits cell division by binding to FtsZ, which is required for septum formation. For this purpose, expression of the chromosomal sulA-lacZ fusion was studied in the ENZ1257 strain without and with colicin M: no induction was detected (data not shown).
While colicins have been assumed to have roles in defense or invasion of an ecological niche, previous studies have shown that colicins are also involved in the promotion of microbial diversity within E. coli populations in the mammalian colon[9, 74]. Furthermore, the nuclease colicins, E9 and E3, have been shown to have the potential to promote microbial genetic diversity via induction of the SOS response or via increased transcription of laterally acquired mobile elements, respectively.
Another study showed that colicins from one producer can induce production in another producer, thus resulting in colicin-mediated colicin induction. Here, we show that subinhibitory concentrations of colicin M induced an envelope and other stress responses including the two component CreBC system connected with increased resistance to colicins M and E2. In natural environments, subinhibitory concentrations of colicin M could thus affect E. coli bacterial communities by promoting ecological adaptation enabling noncolicinogenic cells to survive and compete with colicin producers. The above-described phenomena might also be relevant in the natural settings of other bacterial species, as colicin M homologous proteins have been identified recently in human and plant pathogenic Pseudomonas species that have hydrolytic activity against peptidoglycan precursors. Further, activation of the P. aeruginosa CreBC system has been shown to play a major role in the ß-lactam resistance response.
Resistance of pathogens to traditional antibiotics represents one of the greatest health care threats. The present lack of novel antibiotics is also of great concern. Colicin M has been recently shown to hydrolyse lipid II intermediates of Gram-negative and Gram-positive bacteria. In addition, as the isolated colicin M catalytic domain displays full enzymatic activity, protein engineering can be used to allow binding and translocation in various Gram-negative and Gram-positive species[77, 78]. Furthermore, low concentrations and low protein-to-bacteria ratios suffice for colicin M to kill E. coli. Targeting of lipid II has been indicated as a potential antibacterial strategy.
In conclusion, subinhibitory concentrations of colicin M induced genes involved in adaptive responses to protect the population against envelope and other stresses, including the two component CreBC system associated with increased resistance to some colicins. Our study of the global transcriptional response to colicin M thus provides novel insight into the ecology of colicin M production in natural environments. While an adaptive response was provoked by colicin M treatment there was no induction of biofilm formation, SOS response genes, or other genes involved in mutagenesis, adverse effects shown to be promoted by a number of clinically significant traditional antibiotics. Thus, our study also corroborates the potential of colicin M as a promising antimicrobial and shows the value of microarrays for investigation of novel antimicrobials.
Colicin M expression and isolation
Prior to isolation of colicin M, the cma colicin M structural and cmi immunity genes were PCR amplified from the natural colicin M coding plasmid pCHAP1 using the primers ColM1 5′-TCACTCGAG CATGGAAACCTTAACTGTTCATGCA-3′ and ColM2 5′-CCACGCGT CCACTTCACAGTATGCTCACATTG-3′. The amplified fragment was digested with the Xho I and Mlu I restriction enzymes and cloned into the pET8c expression vector, also cut with the same two enzymes. The isolated plasmid was designated pColM-imm Cloning of cma into the pET8c vector introduced an N-terminal histidine tag with expression under the control of a T7 promotor. Colicin M and the immunity protein were subsequently expressed in E. coli BL21 (DE3)pLysS and colicin M was purified using nickel affinity chromatography[80, 81].
For large scale isolation an overnight culture of BL21 (DE3)pLysS, with plasmid pColM-imm was diluted 100 fold in 500 ml LB with ampicillin (120 μg/ml) and grown at 37°C to an OD600 0.6-0.8, when chromosomal T7 polymerase production was induced by addition of 0.8 mM IPTG and incubated for a further 4 h. Subsequently, cells were harvested and resuspended in 50 mM phosphate, 300 mM NaCl buffer, pH 8, containing RNaseA (20 μg/ml), DNAse (10 μg/ml), lysozyme (1 mg/ml), 10 mM imidazole as well as protein inhibitors and incubated for 1 h at 4°C with shaking. The cells were then lysed with 3 min sonification, 40% amplitude and the supernatant obtained by centrifugation at 17000×g for 1 h at 4°C. The histidine-tag enabled Ni-NTA affinity column purification according to the user’s manual (Qiagen). Nonspecifically bound proteins were washed off the column with 50 mM phosphate, 300 mM NaCl, pH 8.0 buffer, containing 50 mM imidazole while colicin M was subsequently eluted with the same buffer containing 300 mM imidazole.
The colicin-M-containing fractions, as established by 10% SDS-PAGE, were then dialyzed against 5 mM phosphate buffer, pH 7.3, centrifuged at 17,000× g at 4°C for 30 min, and stored at −80°C. Colicin M purity was verified by SDS-PAGE (see Additional file4: Figure S3), and (a concentration of 3.4 mg/ml) protein concentrations were determined using bicinchoninic acid protein assay kits (Pierce) and a Nanodrop ND 1000 spectrophotometer (Thermo Scientific). Finally colicin M was stored at −80°C.
The agar dilution method (National Committee for Clinical Laboratory Standards, 2000) was used to determine the minimal inhibitory concentration (MIC) of colicin M 50 ng/ml. For this purpose, an overnight culture of E. coli MG1655 was diluted 1:625 in LB broth and grown at 37°C with aeration to an OD600 0.6 when the culture was divided into several parts. One part served as a control while the other parts were treated with various concentrations of colicin M. The subinhibitory concentration (30 ng/ml) provoked temporary growth stagnation while growth decline was observed at higher concentrations, presumably due to cell lysis (see Additional file1: Figure S1 in Additional file2: Figure S2).
To investigate the effects of colicin M on the whole genome expression profile, an overnight culture of the E. coli strain MG1655 (F-lambda-ilvG-rfb-50 rph-1) was grown as described above. One part was treated with colicin M (30 ng/ml), while the untreated part served as the control. For gene expression analysis by microarray and qPCR, total RNA was isolated from 2-ml samples removed from each flask following 30 min and 60 min incubations at 37°C. The experiments were repeated at least two times.
For quantification of colanic acid, the growth conditions and the application of subinhibitory concentrations of colicin M were as described above. Colanic acid was purified from 50 ml cultures treated with colicin M for 60 min, 90 min and 120 min at 37°C, with aeration. The experiment was repeated at least two times.
Total RNA was extracted using the RNAProtect Bacteria Reagent (Qiagen) and RNeasy Mini kits (Qiagen), according to the manufacturer instructions. To remove residual DNA, on-column DNase digestion was performed during the RNA purification using the RNase-Free DNase Set (Qiagen). A Nanodrop ND 1000 spectrophotometer (Thermo Scientific) was used to confirm total RNA concentrations, while an Agilent 2100 Bioanalyser (Agilent Technologies, CA, USA) was used to evaluate the RNA quality. The isolated RNA was stored at −80°C until use.
Gene expression analysis was performed using Affymetrix GeneChip® E. coli Genome 2.0 arrays. Target preparation, hybridization, washing, staining and scanning were performed as recommended by the Affymetrix GeneChip® Expression Analysis Technical Manual. The experiment was repeated at least two times.
The acquisition of array images and the data quality assessment were performed using an Affymetrix GeneChip Command Console. The GeneChip data was processed using several different R/Bioconductor packages. The Affymetrix raw data were normalized using the RMA algorithm from the XPS package. The data have been deposited in the NCBI Gene Expression Omnibus database (GEO,http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/geo) under GEO series accession number GSE37026.
Annotation of the genes and the data representation was managed using the ANNAFFY and AFFYCORETOOLS packages. The normalized data, converted to log2 values, were first limited to the ENTREZ-annotated probes from strain K12 (10208 probes). The remaining data were tested for differential expression, which was performed using the LIMMA package for the 30-min treated versus the 30-min untreated control and for the 60-min treated versus the 60-min untreated control bacterial culture. Differential expression was assessed using the 2-way factorial ANOVA model constructed using LIMMA package. Differential expression was assessed using the false discovery rate multiple test correction and controlling type I error at α = 0.05. Differential expression of genes is represented as log2 ratios between the treated and untreated samples averaged over the two biological replicates. Important differentially expressed genes with log2 (fold change) greater than 1 or less than -1 denoting 2-fold up-regulated or down-regulated genes over time were considered for interpretation and are presented in Table 1. The expression of a subset of selected genes was validated by quantitative real-time PCR (qPCR) (see Additional file5: Table S2).
qPCR was performed for 14 genes that showed significant differential expression in the microarray analysis. Samples of 1 μg total RNA were reverse transcribed to synthesize cDNA using High Capacity cDNA Reverse Transcription kits (Applied Biosystems), according to the manufacturer instructions. qPCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems) with an ABI PRISM 7900 HT Sequence Detection System (Applied Biosystems). The qPCR amplifications were performed as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min, and a final dissociation curve analysis step from 60°C to 95°C. Two negative reverse transcription controls were used to show no reverse transcription contamination. qPCR validation was performed on four biological replicates.
Publicly available sequences of the transcripts from the NetAffyx Analysis Centre (http://www.affymetrix.com/analysis/netaffx/index.affx) were analyzed to select target sequences, and the Primer3 software and Primer Express 3.0 software (Applied Biosystems) were used for the design of the specific primers (Sigma). The primer sequences are listed in Additional file5: Table S2.
Raw data were acquired using the Sequence Detection System software, version 2.3 (Applied Biosystems), and gene expression levels were analyzed using the 2-δδCT method, as the efficiency of the qPCR amplifications for all of the genes tested was >90%. geNorm (available from medgen.ugent.be/~jvdesomp/genorm) was used to select the most stable genes, and out of the seven housekeeping genes tested, lpp, aroE, gapA were used as the reference genes, with their geometric mean used for normalization. The results are presented as log2 ratios between gene expression of treated and untreated cultures of four replicates, and they are presented as a comparison with the microarray data (Figure 3).
Colanic acid quantification
Colanic acid was extracted from cultures grown and treated with colicin M as described above, and from untreated control cultures incubated under the same conditions. Colanic acid extraction and quantification was performed as described previously. Briefly, for quantification, the amount of nondialyzable methylpentose ω-deoxyhexose (L-fucose), a component of colanic acid, was measured using a colorimetric reaction with authentic L-fucose (Sigma) as standard, and with concentrations ranging from 5 μg/ml to 100 μg/ml. Colanic acid levels were calculated as μg of nondialyzable ω-deoxyhexose/unit A600 nm/ml of culture.
The ability to form biofilms was investigated using crystal violet assays, as described previously. To assess the induction of biofilm formation, 100 μl of overnight cultures were added to microtiter plates without and with colicin M, and incubated for 24 h at 37°C. The experiments were performed in triplicate.
For quantification, the growth conditions and application of subinhibitory concentrations of colicin M are as described above. The ß-galactosidase activity of the sulA-lacZ gene fusion was measured as described previously.
We thank the Centre for Functional Genomics, at the Medical Faculty, University of Ljubljana, Slovenia, for assistance with the microarray procedures. We also thank S. Gottesman for kindly providing strain MG1655 pATC400, P. Moreau for strain ENZ1257 and A. P. Pugsley for pCHAP1. This study was supported by the Slovenian Research Agency (ARRS) grant P1-0198.
- Goh EB, Yim G, Tsui W, McClure J, Surette MG, Davies J: Transcriptional modulation of bacterial gene expression by subinhibitory concentrations of antibiotics. Proc Natl Acad Sci USA. 2002, 99: 17025-17030. 10.1073/pnas.252607699.PubMedPubMed CentralView ArticleGoogle Scholar
- Davies J, Spiegelman GB, Yim G: The world of subinhibitory antibiotic concentrations. Curr Opin Microbiol. 2006, 9: 445-453. 10.1016/j.mib.2006.08.006.PubMedView ArticleGoogle Scholar
- Braun V, Patzer SI, Hantke K: Ton-dependent colicins and microcins: modular design and evolution. Biochimie. 2002, 84: 365-380. 10.1016/S0300-9084(02)01427-X.PubMedView ArticleGoogle Scholar
- El Ghachi M, Bouhss A, Barreteau H, Touzé T, Auger G, Blanot D, Mengin-Lecreulx D: Colicin M exerts its bacteriolytic effect via enzymatic degradation of undecaprenyl phosphate-linked peptidoglycan precursors. J Biol Chem. 2006, 281: 22761-22772. 10.1074/jbc.M602834200.PubMedView ArticleGoogle Scholar
- Harkness RE, Olschläger T: The biology of colicin M. FEMS Microbiol Rev. 1991, 8: 27-41.PubMedGoogle Scholar
- Sasarman A, Massie B, Zollinger M, Gagnetellier H, Shareck F, Garzon S, Morisset R: Naturally occurring R. ColBM plasmids belonging to the IncfIII incompatibility group. J Genl Microbiol. 1980, 119: 475-483.Google Scholar
- Christenson JK, Gordon DM: Evolution of colicin BM plasmids: the loss of the colicin B activity gene. Microbiology. 2009, 155: 1645-1655. 10.1099/mic.0.026666-0.PubMedView ArticleGoogle Scholar
- Kerr B, Riley MA, Feldman MW, Bohannan BJM: Local dispersal promotes biodiversity in a real-life game of rock-paper-scissors. Nature. 2002, 418: 171-174. 10.1038/nature00823.PubMedView ArticleGoogle Scholar
- Kirkup BC, Riley MA: Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo. Nature. 2004, 428: 412-414. 10.1038/nature02429.PubMedView ArticleGoogle Scholar
- Lautenbach E, Patel JB, Bilker WB, Edelstein PH, Fishman NO: Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae: risk factors for infection and impact of resistance on outcomes. Clin Infect Dis. 2001, 32: 1162-1171. 10.1086/319757.PubMedView ArticleGoogle Scholar
- Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R, Chaudhary U, Doumith M, Giske CG, Irfan S, Krishnan P, Kumar AV, Maharjan S, Mushtaq S, Noorie T, Paterson DL, Pearson A, Perry C, Pike R, Rao B, Ray U, Sarma JB, Sharma M, Sheridan E, Thirunarayan MA, Turton J, Upadhyay S, Warner M, Welfare W, Livermore DM: Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. 2010, 10: 597-602. 10.1016/S1473-3099(10)70143-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Patin D, Barreteau H, Auger G, Magnet S, Crouvoisier M, Bouhss A, Touze T, Arthur M, Mengin-Lecreulx D, Blanot D: Colicin M hydrolyses branched lipids II from Gram-positive bacteria. Biochimie. 2012, 94: 985-990. 10.1016/j.biochi.2011.12.019.PubMedView ArticleGoogle Scholar
- Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997, 277: 1453-1462. 10.1126/science.277.5331.1453.PubMedView ArticleGoogle Scholar
- Nikaido H: Outer membrane. Escherichia coli and Salmonella: Cellular and Molecular biology. Volume 1. Edited by: Neidhardt FC, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. 1996, Washington, DC: ASM Press, 29-47. 2Google Scholar
- Kadner RJ: Cytoplasmic membrane. Escherichia coli and Salmonella: Cellular and Molecular biology. Volume 1. Edited by: Neidhardt FC, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. 1996, Washington, DC: ASM Press, 58-87. 2Google Scholar
- Casino P, Rubio V, Marina A: The mechanism of signal transduction by two-component systems. Curr Opin Struct Biol. 2010, 20: 763-771. 10.1016/j.sbi.2010.09.010.PubMedView ArticleGoogle Scholar
- Coyette J, Van Der Ende A: Peptidoglycan: the bacterial Achilles heel. FEMS Microbiol Rev. 2008, 32: 147-148. 10.1111/j.1574-6976.2008.00108.x.PubMedView ArticleGoogle Scholar
- Bury-Moné S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P: Global analysis of extracytoplasmic stress signaling in Escherichia coli. PLoS Genet. 2009, 5: e1000651-10.1371/journal.pgen.1000651.PubMedPubMed CentralView ArticleGoogle Scholar
- McBroom AJ, Kuehn MJ: Release of outer membrane vesicles by gram-negative bacteria is a novel envelope stress response. Mol Microbiol. 2007, 63: 545-558.PubMedPubMed CentralView ArticleGoogle Scholar
- Ruiz N, Silhavy TJ: Sensing external stress: watchdogs of the Escherichia coli cell envelope. Curr Opin Microbiol. 2005, 8: 122-126. 10.1016/j.mib.2005.02.013.PubMedView ArticleGoogle Scholar
- Stout V, Torres-Cabassa A, Maurizi MR, Gutnick D, Gottesman S: RcsA, an unstable positive regulator of capsular polysaccharide synthesis. J Bacteriol. 1991, 173: 1738-1747.PubMedPubMed CentralGoogle Scholar
- Rahn A, Whitfield C: Transcriptional organization and regulation of the Escherichia coli K30 group 1 capsule biosynthesis (cps) gene cluster. Mol Microbiol. 2003, 47: 1045-1060. 10.1046/j.1365-2958.2003.03354.x.PubMedView ArticleGoogle Scholar
- Ferrières L, Clarke DJ: The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface. Mol Microbiol. 2003, 50: 1665-1682. 10.1046/j.1365-2958.2003.03815.x.PubMedView ArticleGoogle Scholar
- Prigent-Combaret C, Prensier G, Le Thi TT, Vidal O, Lejeune P, Dorel C: Developmental pathway for biofilm formation in curli-producing Escherichia coli strains: role of flagella, curli and colanic acid. Environ Microbiol. 2000, 2: 450-464. 10.1046/j.1462-2920.2000.00128.x.PubMedView ArticleGoogle Scholar
- Francez-Charlot A, Castanié-Cornet MP, Gutierrez C, Cam K: Osmotic regulation of the Escherichia coli bdm (biofilm-dependent modulation) gene by the RcsCDB His-Asp phosphorelay. J Bacteriol. 2005, 187: 3873-3877. 10.1128/JB.187.11.3873-3877.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Laubacher ME, Ades SE: The Rcs phosphorelay is a cell envelope stress response activated by peptidoglycan stress and contributes to intrinsic antibiotic resistance. J Bacteriol. 2008, 190: 2065-2074. 10.1128/JB.01740-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Callewaert L, Vanoirbeek KGA, Lurquin I, Michiels CW, Aertsen A: The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme. J Bacteriol. 2009, 191: 1979-1981. 10.1128/JB.01549-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Raivio TL, Popkin DL, Silhavy TJ: The Cpx envelope stress response is controlled by amplification and feedback inhibition. J Bacteriol. 1999, 181: 5263-5272.PubMedPubMed CentralGoogle Scholar
- Joly N, Engl C, Jovanovic G, Huvet M, Toni T, Sheng X, Stumpf MPH, Buck M: Managing membrane stress: the phage shock protein (Psp) response, from molecular mechanisms to physiology. FEMS Microbiol Rev. 2010, 34: 797-827.PubMedView ArticleGoogle Scholar
- Kobayashi H, Yamamoto M, Aono R: Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents. Microbiology. 1998, 144: 353-359. 10.1099/00221287-144-2-353.PubMedView ArticleGoogle Scholar
- Jovanovic G, Lloyd LJ, Stumpf MPH, Mayhew AJ, Buck M: Induction and function of the phage shock protein extracytoplasmic stress response in Escherichia coli. J Biol Chem. 2006, 281: 21147-21161. 10.1074/jbc.M602323200.PubMedView ArticleGoogle Scholar
- Kobayashi R, Suzuki T, Yoshida M: Escherichia coli phage-shock protein A (PspA) binds to membrane phospholipids and repairs proton leakage of the damaged membranes. Mol Microbiol. 2007, 66: 100-109. 10.1111/j.1365-2958.2007.05893.x.PubMedView ArticleGoogle Scholar
- Gottesman S, Stout V: Regulation of capsular polysaccharide synthesis in Escherichia coli K12. Mol Microbiol. 1991, 5: 1599-1606. 10.1111/j.1365-2958.1991.tb01906.x.PubMedView ArticleGoogle Scholar
- Agladze K, Wang X, Romeo T: Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA. J Bacteriol. 2005, 187: 8237-8246. 10.1128/JB.187.24.8237-8246.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Sledjeski DD, Gottesman S: Osmotic shock induction of capsule synthesis in Escherichia coli K-12. J Bacteriol. 1996, 178: 1204-1206.PubMedPubMed CentralGoogle Scholar
- Stevenson G, Andrianopoulos K, Hobbs M, Reeves PR: Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J Bacteriol. 1996, 178: 4885-4893.PubMedPubMed CentralGoogle Scholar
- Böhringer J, Fischer D, Mosler G, Henggearonis R: UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli. J Bacteriol. 1995, 177: 413-422.PubMedPubMed CentralGoogle Scholar
- Ferrières L, Aslam SN, Cooper RM, Clarke DJ: The yjbEFGH locus in Escherichia coli K-12 is an operon encoding proteins involved in exopolysaccharide production. Microbiology. 2007, 153: 1070-1080. 10.1099/mic.0.2006/002907-0.PubMedView ArticleGoogle Scholar
- Ionescu M, Belkin S: Overproduction of exopolysaccharides by an Escherichia coli K-12 rpoS mutant in response to osmotic stress. Appl Environ Microbiol. 2009, 75: 483-492. 10.1128/AEM.01616-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Cariss SJL, Tayler AE, Avison MB: Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli. J Bacteriol. 2008, 190: 3930-3939. 10.1128/JB.00108-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Helbig S, Hantke K, Ammelburg M, Braun V: CbrA is a flavin adenine dinucleotide protein that modifies the Escherichia coli outer membrane and confers specific resistance to colicin M. J Bacteriol. 2012, 194: 4894-4903. 10.1128/JB.00782-12.PubMedPubMed CentralView ArticleGoogle Scholar
- Kuhar I, Žgur-Bertok D: Transcription regulation of the colicin K cka gene reveals induction of colicin synthesis by differential responses to environmental signals. J Bacteriol. 1999, 181: 7373-7380.PubMedPubMed CentralGoogle Scholar
- Eraso JM, Chidambaram M, Weinstock GM: Increased production of colicin E1 in stationary phase. J Bacteriol. 1996, 178: 1928-1935.PubMedPubMed CentralGoogle Scholar
- Moya B, Dötsch A, Juan C, Blázquez J, Zamorano L, Haussler S, Oliver A: Beta-lactam resistance response triggered by inactivation of a nonessential penicillin-binding protein. PLoS Pathog. 2009, 5: e1000353-10.1371/journal.ppat.1000353.PubMedPubMed CentralView ArticleGoogle Scholar
- Jung JU, Gutierrez C, Martin F, Ardourel M, Villarejo M: Transcription of osmB, a gene encoding an Escherichia coli lipoprotein, is regulated by dual signals. Osmotic stress and stationary phase. J Biol Chem. 1990, 265: 10574-10581.PubMedGoogle Scholar
- Lee J, Hiibel SR, Reardon KF, Wood TK: Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene. J Appl Microbiol. 2010, 108: 2088-2102.PubMedGoogle Scholar
- Ratajczak E, Ziętkiewicz S, Liberek K: Distinct activities of Escherichia coli small heat shock proteins IbpA and IbpB promote efficient protein disaggregation. J Mol Biol. 2009, 386: 178-189. 10.1016/j.jmb.2008.12.009.PubMedView ArticleGoogle Scholar
- Flemming H-C, Wingender J: The biofilm matrix. Nat Rev Micro. 2010, 8: 623-633.Google Scholar
- Costerton JW, Stewart PS, Greenberg EP: Bacterial biofilms: a common cause of persistent infections. Science. 1999, 284: 1318-1322. 10.1126/science.284.5418.1318.PubMedView ArticleGoogle Scholar
- Danese PN, Pratt LA, Kolter R: Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture. J Bacteriol. 2000, 182: 3593-3596. 10.1128/JB.182.12.3593-3596.2000.PubMedPubMed CentralView ArticleGoogle Scholar
- Boehm A, Vogel J: The csgD mRNA as a hub for signal integration via multiple small RNAs. Mol Microbiol. 2012, 84: 1-5. 10.1111/j.1365-2958.2012.08033.x.PubMedView ArticleGoogle Scholar
- Mika F, Busse S, Possling A, Berkholz J, Tschowri N, Sommerfeldt N, Pruteanu M, Hengge R: Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli. Mol Microbiol. 2012, 84: 51-65. 10.1111/j.1365-2958.2012.08002.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Holmqvist E, Reimegård J, Sterk M, Grantcharova N, Römling U, Wagner EGH: Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis. EMBO J. 2010, 29: 1840-1850. 10.1038/emboj.2010.73.PubMedPubMed CentralView ArticleGoogle Scholar
- Sim SH, Yeom JH, Shin C, Song WS, Shin E, Kim HM, Cha CJ, Han SH, Ha NC, Kim SW, Hahn Y, Bae J, Lee K: Escherichia coli ribonuclease III activity is downregulated by osmotic stress: consequences for the degradation of bdm mRNA in biofilm formation. Mol Microbiol. 2010, 75: 413-425. 10.1111/j.1365-2958.2009.06986.x.PubMedView ArticleGoogle Scholar
- Jonas K, Edwards AN, Simm R, Romeo T, Römling U, Melefors Ö: The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins. Mol Microbiol. 2008, 70: 236-257. 10.1111/j.1365-2958.2008.06411.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Price NL, Raivio TL: Characterization of the Cpx regulon in Escherichia coli strain MC4100. J Bacteriol. 2009, 191: 1798-1815. 10.1128/JB.00798-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Yamamoto K, Ishihama A: Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli. Biosci Biotechnol Biochem. 2006, 70: 1688-1695. 10.1271/bbb.60024.PubMedView ArticleGoogle Scholar
- Wang X, Preston JF, Romeo T: The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation. J Bacteriol. 2004, 186: 2724-2734. 10.1128/JB.186.9.2724-2734.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Soutourina OA, Bertin PN: Regulation cascade of flagellar expression in Gram-negative bacteria. FEMS Microbiol Rev. 2003, 27: 505-523. 10.1016/S0168-6445(03)00064-0.PubMedView ArticleGoogle Scholar
- Shi W, Li C, Louise CJ, Adler J: Mechanism of adverse conditions causing lack of flagella in Escherichia coli. J Bacteriol. 1993, 175: 2236-2240.PubMedPubMed CentralGoogle Scholar
- Soutourina OA, Krin E, Laurent-Winter C, Hommais F, Danchin A, Bertin PN: Regulation of bacterial motility in response to low pH in Escherichia coli: the role of H-NS protein. Microbiology. 2002, 148: 1543-1551.PubMedView ArticleGoogle Scholar
- Torres-Cabassa AS, Gottesman S: Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J Bacteriol. 1987, 169: 981-989.PubMedPubMed CentralGoogle Scholar
- Yim G, Huimi Wang H, Davies J: The truth about antibiotics. Int J Med Microbiol. 2006, 296: 163-170. 10.1016/j.ijmm.2006.01.039.PubMedView ArticleGoogle Scholar
- Fajardo A, Martinez JL: Antibiotics as signals that trigger specific bacterial responses. Curr Opin Microbiol. 2008, 11: 161-167. 10.1016/j.mib.2008.02.006.PubMedView ArticleGoogle Scholar
- Sailer FC, Meberg BM, Young KD: β-Lactam induction of colanic acid gene expression in Escherichia coli. FEMS Microbiol Lett. 2003, 226: 245-249. 10.1016/S0378-1097(03)00616-5.PubMedView ArticleGoogle Scholar
- Kaldalu M, Mei R, Lewis K: Killing by ampicillin and ofloxacin induces overlapping changes in Escherichia coli transcription profile. Antimicrob Agents Chemother. 2004, 48: 890-896. 10.1128/AAC.48.3.890-896.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Subrt N, Mesak LR, Davies J: Modulation of virulence gene expression by cell wall active antibiotics in Staphylococcus aureus. J Antimicrob Chemother. 2011, 66: 979-984. 10.1093/jac/dkr043.PubMedView ArticleGoogle Scholar
- Hoffman LR, D’Argenio DA, MacCoss MJ, Zhang Z, Jones RA, Miller SI: Aminoglycoside antibiotics induce bacterial biofilm formation. Nature. 2005, 436: 1171-1175. 10.1038/nature03912.PubMedView ArticleGoogle Scholar
- Linares JF, Gustafsson I, Baquero F, Martinez JL: Antibiotics as intermicrobial signaling agents instead of weapons. Proc Natl Acad Sci USA. 2006, 103: 19484-19489. 10.1073/pnas.0608949103.PubMedPubMed CentralView ArticleGoogle Scholar
- Ohmori H, Friedberg EC, Fuchs RP, Goodman MF, Hanaoka F, Hinkle D, Kunkel TA, Lawrence CW, Livneh Z, Nohmi T, Prakash L, Prakash S, Todo T, Walker GC, Wang Z, Woodgate R: The Y-family of DNA polymerases. Mol Cell. 2001, 8: 7-8. 10.1016/S1097-2765(01)00278-7.PubMedView ArticleGoogle Scholar
- Kohanski MA, DePristo MA, Collins JJ: Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis. Mol Cell. 2010, 37: 311-320. 10.1016/j.molcel.2010.01.003.PubMedPubMed CentralView ArticleGoogle Scholar
- Thi DT, López E, Rodríguez-Rojas A, Rodríguez-Beltrán J, Couce A, Guelfo JR, Castañeda-García A, Blázquez J: Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials. J Antimicrob Chemother. 2011, 66: 531-538. 10.1093/jac/dkq496.PubMedView ArticleGoogle Scholar
- Moreau PL: Diversion of the metabolic flux from pyruvate dehydrogenase to pyruvate oxidase decreases oxidative stress during glucose metabolism in nongrowing Escherichia coli cells incubated under aerobic, phosphate starvation conditions. J Bacteriol. 2004, 186: 7364-7368. 10.1128/JB.186.21.7364-7368.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Majeed H, Gillor O, Kerr B, Riley MA: Competitive interactions in Escherichia coli populations: the role of bacteriocins. ISME J. 2011, 5: 71-81. 10.1038/ismej.2010.90.PubMedPubMed CentralView ArticleGoogle Scholar
- Walker D, Rolfe M, Thompson A, Moore GR, James R, Hinton JCD, Kleanthous C: Transcriptional profiling of colicin-induced cell death of Escherichia coli MG1655 identifies potential mechanisms by which bacteriocins promote bacterial diversity. J Bacteriol. 2004, 186: 866-869. 10.1128/JB.186.3.866-869.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Barreteau H, Bouhss A, Fourgeaud M, Mainardi JL, Touzé T, Gérard F, Blanot D, Arthur M, Mengin-Lecreulx D: Human- and plant-pathogenic Pseudomonas species produce bacteriocins exhibiting colicin M-like hydrolase activity towards peptidoglycan precursors. J Bacteriol. 2009, 191: 3657-3664. 10.1128/JB.01824-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Barnéoud-Arnoulet A, Barreteau H, Touzé T, Mengin-Lecreulx D, Lloubès R, Duché D: Toxicity of the colicin M catalytic domain exported to the periplasm is FkpA independent. J Bacteriol. 2010, 192: 5212-5219. 10.1128/JB.00431-10.PubMedPubMed CentralView ArticleGoogle Scholar
- Barreteau H, Bouhss A, Gérard F, Duché D, Boussaid B, Blanot D, Lloubès R, Mengin-Lecreulx D, Touzé T: Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme. J Biol Chem. 2010, 285: 12378-12389. 10.1074/jbc.M109.093583.PubMedPubMed CentralView ArticleGoogle Scholar
- Breukink E, de Kruijff B: Lipid II as a target for antibiotics. Nat Rev Drug Discov. 2006, 5: 321-323. 10.1038/nrd2004.PubMedView ArticleGoogle Scholar
- Budič M, Rijavec M, Petkovšek Ž, Žgur-Bertok D: Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia. PLoS One. 2011, 6: e28769-10.1371/journal.pone.0028769.PubMedPubMed CentralView ArticleGoogle Scholar
- Anderluh G, Gökçe I, Lakey JH: Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner. Protein Expr Purif. 2003, 28: 173-181. 10.1016/S1046-5928(02)00681-2.PubMedView ArticleGoogle Scholar
- Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc B Met. 1995, 57: 289-300.Google Scholar
- Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.PubMedGoogle Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔC T method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.PubMedView ArticleGoogle Scholar
- Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: RESEARCH0034-PubMedPubMed CentralView ArticleGoogle Scholar
- Obadia B, Lacour S, Doublet P, Baubichon-Cortay H, Cozzone AJ, Grangeasse C: Influence of tyrosine-kinase Wzc activity on colanic acid production in Escherichia coli K12 Cells. J Mol Biol. 2007, 367: 42-53. 10.1016/j.jmb.2006.12.048.PubMedView ArticleGoogle Scholar
- Rijavec M, Müller-Premru M, Zakotnik B, Žgur-Bertok D: Virulence factors and biofilm production among Escherichia coli strains causing bacteraemia of urinary tract origin. J Medical Microbiol. 2008, 57: 1329-1334. 10.1099/jmm.0.2008/002543-0.View ArticleGoogle Scholar
- Miller JH: Experiments in molecular genetics: Assay of β-galactosidase. 1972, Cold Spring Harbor: CSH Laboratory Press, 352-355.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.