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Fig. 3 | BMC Microbiology

Fig. 3

From: High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran

Fig. 3

A) Simplex PCR for blaTEM gene (800 bp); M: DNA ladder (100 bp); Lanes 1: positive control; Lane 2: negative control: DNA/RNA free water; Lanes 3 and 6: Enterobacter isolates positive for blaTEM gene; Lanes 2,4,5, and 7: isolates negative for blaTEM gene. B) Simplex PCR for blaSHV gene (843 bp); M: DNA ladder (100 bp); Lanes 1: negative control: DNA/RNA free water; Lane 2: positive control; Lanes 3 to 11: Enterobacter isolates positive for blaSHV gene; Lanes 12: Enterobacter isolate negative for blaSHV gene. C) Simplex PCR for blaCTX−M−14 gene (120 bp); M: DNA ladder (100 bp); Lanes 1: positive control; Lane 2: negative control: DNA/RNA free water; Lanes 3 and 4: Enterobacter isolates negative for blaCTX−M−14 gene; Lanes 5 to 9: isolates positive for blaCTX−M−14 gene. D) Simplex PCR for blaEBC gene (302 bp); M: DNA ladder (100 bp); Lanes 1: negative control: DNA/RNA free water; Lane 2: positive control; Lanes 3 to 7: Enterobacter isolates positive for blaEBC gene

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