Fig. 1
From: The distinct cell physiology of Bradyrhizobium at the population and cellular level
General features ofBradyrhizobium growth.(a) A representative growth curve manually performed by following OD450nm along time in classical YEM medium of B. japonicum E109 (Blue), B. japonicum USDA6T (Red), B. diazoefficiens USDA110 (green) and B. diazoefficiens USDA122 (purple). (b) Colony Forming Units per mL from a) of culture are plotted as a function of time. (c) Photograph of typical cell aggregates observed for the four isolates that spontaneously appear during the first 48hs accompanying the OD reduction (left). Microscopy observation of B. japonicum E109 aggregates using 1000X magnification (right). The photograph shows the cellular nature of aggregates. The black bar indicates 10 μm. (d) Generation times were calculated from several manual growth curves. The graph shows the box & whiskers plot minimum to maximum showing all the individual values. The central line indicates the median (n ≥ 6). Statistical significance was analyzed using Kruskal-Wallis non-parametric tests followed by Dunn’s multiple comparisons. *** means p < 0.001