Strain | Chromosomal phoP statusa, b | Chromosomal pmrB status | Episomal pmrB status | Relative expression level (mean ± SD) | Colistin MIC (µg/mL) | ||||
---|---|---|---|---|---|---|---|---|---|
phoP | pmrD | pmrA | pmrB | pmrK | |||||
MG1655 | WT | WT | None | 1 | 1 | 1 | 1 | 1 | 0.5 |
TSAREC03 | g252a (premature termination) | g3c, t41c, c532t, c704a (M1I, L14P,P178S,T235N) | None | 0.17 ± 0.08 | 0.59 ± 0.24 | 116.79 ± 21.12 | 38.18 ± 10.46 | 146.32 ± 98.16 | 8 |
TSAREC03_ΔphoPΔ84–224 | Deleted | g3c, t41c, c532t, c704a (M1I, L14P,P178S,T235N) | None | ND | ND | ND | ND | ND | 8 |
TSAREC03 WT-phoP revertant | WT | g3c, t41c, c532t, c704a (M1I, L14P,P178S,T235N) | None | 0.56 ± 0.41 | 1.44 ± 0.54 | 106.04 ± 41.17 | 32.53 ± 17.84 | 112.03 ± 61.87 | 8 |
TSAREC03_ΔpmrBg3c, t41c, c532t, c704a | g252a (premature termination) | Deleted | None | ND | ND | 4.66 ± 0.56 | < 0.01 | 2.74 ± 0.71 | 0.25 |
TSAREC03 _ΔpmrBg3c, t41c, c532t, c704a (pCRII-TOPOpmrBg3c, t41c, c532t, c704a) | g252a (premature termination) | Deleted | g3c, t41c, c532t, c704a (M1I, L14P,P178S,T235N) | ND | ND | 157.00 ± 43.61 | 66.52 ± 44.80 | 251.70 ± 133.80 | 8 |