Strain | Chromosomal mgrB statusa,b | Chromosomal pmrB status | Episomal pmrB status | Relative expression level (mean ± SD) | Colistin MIC(µg/mL) | ||||
---|---|---|---|---|---|---|---|---|---|
phoP | pmrD | pmrA | pmrB | pmrK | |||||
MG1655 | WT | WT | None | 1 | 1 | 1 | 1 | 1 | 0.5 |
TSAREC02 | c127t (premature termination) | g616a, t618g, t664c (G206R, Y222H.) | None | 1.92 ± 0.68 | 0.77 ± 0.17 | 0.11 ± 0.03 | 5.26 ± 2.65 | 63.04 ± 39.34 | 16 |
TSAREC02_ΔmgrBΔ43–47 | Deleted | g616a, t618g, t664c (G206R, Y222H.) | None | ND | ND | ND | ND | ND | 16 |
TSAREC02 WT-mgrB revertant | WTb | g616a, t618g, t664c (G206R, Y222H.) | None | 0.88 ± 0.43 | 0.32 ± 0.09 | 0.35 ± 0.28 | 4.48 ± 2.47 | 53,65 ± 46.35 | 16 |
TSAREC02 _ΔpmrBg616a, t618g, t664c | c127t (premature termination) | Deleted | None | ND | ND | 0.01 ± 0.001 | < 0.01 | 3.08 ± 0.88 | 0.5 |
TSAREC02 _ΔpmrBg616a, t618g, t664c (pCRII-TOPOpmrBg616a, t618g, t664c) | c127t (premature termination) | Deleted | g616a, t618g, t664c (G206R, Y222H.) | ND | ND | 0.12 ± 0.03 | 18.54 ± 4.22 | 83.41 ± 22.63 | 16 |