Fig. 4From: The potential role of Listeria monocytogenes in promoting colorectal adenocarcinoma tumorigenic processEffects of HK L. monocytogenes on CaCo2 cell tumorigenic potential in anchorage-independent conditions. CaCo2 cells were cultured in soft agar layer in contact with 1:10, 1:20, or 1:50 cell-to-HK L. monocytogenes ratios, or with 1:10, 1:20 or 1:50 cell-to-HK L. innocua ratios, for 31 days. Representative stereoscope images of 3D colony formation (A). Cell tumorigenic potential was assessed by counting the three-dimensional colonies formed. Data show the mean results of four different experiments; results are expressed as the total number of colonies ± standard error (B). Statistical significance is compared to CTR. *** p < 0.001; one-way ANOVA, followed by Dunnett’s Multiple Comparison Test. HK Lm: heat-killed L. monocytogenes; HK Li: heat-killed L. innocuaBack to article page