Table 1 Seven potentially lytic enzymes were identified in S. carnosus TMW 2.146 by comparative genomics. Their locus tag, annotation, number of amino acids, and predicted molecular weight are given in the table. Four of the seven putative cell wall hydrolases were selected to perform a knockout. (+) knockout mutant available, (−) no knockout mutant available, (*) were not considered for the knockout
From: Characterization of the major autolysin (AtlC) of Staphylococcus carnosus
Locus tag (SAMN04563519) | Annotation | Number of amino acids | Molecular weight | Strain number | |
|---|---|---|---|---|---|
TMW 2.146 | TMW 2.2515 | ||||
A2I62_03315 | LytD, Beta- N-acetylglucosaminidase | 259 | 30.08 kDa | -* | -* |
A2I62_05625 | LytD, Beta- N-acetylglucosaminidase | 281 | 32.22 kDa | -* | -* |
A2I62_09095 | Bifunctional autolysin Atl / N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)/ endo-beta-N-acetylglucosaminidase (EC 3.2.1.96) | 1254 | 136.25 kDa | + | + |
A2I62_12025 | 1,4-beta-N-acetylmuramoylhydrolase | 338 | 35.91 kDa | – | – |
A2I62_00235 | N-acetylmuramoyl-L-alanine amidase domain-containing protein | 708 | 80.07 kDa | + | + |
A2I62_06240 | cell wall amidase LytH | 291 | 32.7 kDa | -* | -* |
A2I62_09805 | N-acetylmuramoyl-L-alanine amidase | 467 | 52.91 kDa | – | – |