Fig. 8

Zymograms of S. carnosus TMW 2.146 and TMW 2.2515 and their respective atlC mutants. Samples were taken from the supernatant of the respective cultures over 7 days. These samples were sterile-filtered and subsequently denatured by Laemmli loading dye. The denatured proteins were separated by size in a zymogram. Later, the proteins were renatured using a renaturation buffer (50 mM Tris -HCl pH 8 with 0.5% Triton X-100). Lytic proteins cleave Micrococcus lysodeikticus cells in the gel and form bright bands in the otherwise gray gel. First, many bands are formed in the wild-type strains, and no bands are visible in the knockout mutants. Furthermore, one can see that the banding pattern of the wild-type strains changes over the 7 days