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Fig. 4 | BMC Microbiology

Fig. 4

From: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium

Fig. 4

Enzymatic properties of ZEA-degrading enzymes. A: SDS‒PAGE analysis of the purified proteins. M: Marker; 1: crude ZHDR52; 2: 1st eluent of crude ZHDR52; 3: 2nd eluent of crude ZHDR52; 4: purified ZHDR52; 5: crude ZHDP83; 6: 1st eluent of crude ZHDP83; 7: 2nd eluent of crude ZHDP83; 8: purified ZHDP83; 9: crude ZHD101; 10: 1st eluent of crude ZHD101; 11: 2nd eluent of crude ZHD101; 12: purified ZHD101. B: Effect of pH on the activity of ZEA-degrading enzymes. C: Effects of temperature on the activity of ZEA-degrading enzymes. D: Relative activity of the ZEA-degrading enzymes. The data are shown as the mean ± SEM (n = 3). Dunnett’s multiple comparisons test was used for multiple comparisons, **** p < 0.0001

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