Fig. 3
Temperature optimization for HCV-RT-LAMP amplification. The RT-LAMP amplifications for detection of HCV were monitored using real-time turbidity (LA-500, Eiken Chemical Co., Ltd., Japan). The corresponding curves of amplicons are displayed in the graphs. Turbidity > 0.1 indicated a positive value. Eight kinetic graphs were obtained at different temperatures (63–70 °C, 1 °C intervals) with 2 × 103 copies of the target gene (A-H). The graphs from E (67 °C) showed robust amplification