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Table 3 Correlation between the expected and observed proportions of relative abundance at the genus level and the number of PCR cycles. Correlations were calculated using the Pearson’s correlation coefficient

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16S Databases

Silva

RefSeq

Workflows

Kraken2

EPI2ME

BugSeq

16S

WIMP

PCR cycles

15

0.73

0.77

0.46

0.79

20

0.74

0.77

0.45

0.78

25

0.74

0.78

0.45

0.79

30

0.74

0.77

0.47

0.75

35

0.73

0.76

0.49

0.58

  1. The Epi2me and 16S workflow had a minimum identification accuracy of 80%. PCR was conducted using an annealing temperature of 48°C, primer set#1, and LongAmp Taq polymerase
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BMC Microbiology

ISSN: 1471-2180

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