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Fig. 3 | BMC Microbiology

Fig. 3

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

Fig. 3

The hierarchical clustering heat map shows the relative abundance of each genus in the microbial mock community using primer sets #1 and #2 at different annealing temperatures (48, 50, and 52°C) and Taq polymerases (LongAmp and iTaq). The results were analysed using BugSeq, and the bottom row of the table shows the Pearson correlation coefficient. The results were compared with the expected (theoretical) proportion of the mock community. The coloured gradient legend represents a linear scale of relative abundance. (others = misclassified sequences)

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