Fig. 1

Schematic diagram of the basic principle and process of nanopore sequencing workflow. DNA from a pre-set microbial community is used as a template for 16S rRNA gene amplification by polymerase chain reaction (PCR). We analysed the influence of the type of Taq polymerase, annealing temperature, type of primers, and number of cycles used during the reactions. Following a standard library preparation process, the 16S gene DNA fragment was subjected to sequencing using a nanopore sequencer (MinION), and the results were then screened and processed according to the workflows, databases and accuracy settings. (Diagram adapted from [22])