Table 1 Assay evaluation using clinical respiratory samples. Ten clinical respiratory samples were assessed using TaqMan qPCR to determine Aspergillus fumigatus positivity. Two samples were negative for A. fumigatus (labelled QN). Three samples contained A. fumigatus DNA at a positivity level below 1 GE per reaction, and 5 samples at a positivity level above 1 GE per reaction (shown in bold). All samples above the sequencing assay’s theoretical detection limit (1 GE/reaction) (n=5) were identified as A. fumigatus positive by TEF sequencing

From: Development of a novel mycobiome diagnostic for fungal infection