Fig. 5

TEF species detection in mock fungal communities is not significantly hindered by human gDNA background. A. Boxplot of percent abundance for species spiked at 2% within mock communities targeted by TEF. Expected percent abundance is indicated by dashed black line. Dotted red line indicates no identification. Percent abundances are grouped by human DNA background status. Species level quantifications were significantly different when with and without human background for (A) terreus, C. auris and C. albicans (*; P < 0.05 or ***; P < 0.0001 by Wilcoxon rank sum test). For all other species which were identified in a sample, quantifications did not differ significantly (P < 0.05 by Wilcoxon rank sum test). Wilcoxon rank sum tests were not performed for species with less than 3 data points per human background status and are indicated with ‘ND’. (B) Heatmap representation of TEF identification (ID) rates for species when spiked at 2% within mock communities depending on human DNA background status. Human DNA resulted in no change for most species tested