Fig. 6
From: A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation
Structure of Ngo-Lig E. (A) Overall structure of DNA-engaged Ngo-Lig E coloured by domain shown as a cartoon (i) or as a surface (ii). (B) View of the NTase domain active site including the adenylated catalytic lysine residue. (C) Position of the disulphide bond in the OB domain. (D) Inter-domain interactions surrounding the linker. Electrostatic and polar interactions less than 5Å are indicated by dashed yellow lines. (E) Interaction of Ngo-Lig E with DNA duplex (i), specific interactions between conserved residues and the ‘complement’ strand of the duplex (ii), specific interactions between residues of the OB domain and the equivalent of the ‘nicked’ strand where the DNA break is located in other DNA-bound ligase structures (iii)