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Table 1 The set of MCDA primers used in this study

From: Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes

Primers name

Sequences

Length

gene

speB-F1

5'-CGGAAGAGAATCTAACGTTCAA-3'

22 nt*1

speB

speB-F2

5'-GTATACTGGTTGGTTTTGAGAT-3'

22 nt

 

speB-CP1

5'-CCACTAGATGGACCATAATCCATGTGCGATTTCAGAATTGATGGC-3'

45 mer *2

 

speB-CP2

5'-CTTTGGCTACAACCAATCTGTTCATTCTTTGTCAATTTGTGCTTC-3'

45 mer

 

speB-C1

5'-CCACTAGATGGACCATAATCCATGT-3'

25 nt

 

speB-C2

5'-CTTTGGCTACAACCAATCTGTTCA-3'

24 nt

 

speB-D1

5'-CTACTGAAATACCAACATCA-3'

20 nt

 

speB-D1*3

5'-FITC-CTACTGAAATACCAACATCA-3'

20 nt

 

speB-D2

5'-GCGACTTTAGCAAACAAGAT-3'

20 nt

 

speB-R1

5'-AGAGCTACCTGCAGAA-3'

16nt

 

speB-R1*4

5'-Biotin-AGAGCTACCTGCAGAA-3'

16nt

 

speB-R2

5'-GTGTTCAAAGAGCCTTGAA-3'

19 nt

 
  1. 1 nt, nucleotide;
  2. 2 mer, monomeric;
  3. 3 D1*, 5ʹ-labeled with fluorescein (FITC);
  4. 4 R1*, 5ʹ-labeled with biotin;