Table 2 Coded values of the Plackett-Burman Design for epothilone production by Aspergillus fumigatus under solid state fermentation

code

variable

-1

+1

X1

Starch (g/L)

2

5

X2

Dextrin (g/L)

2

4

X3

Lactose (g/L)

1

3

X4

Sucrose (g/L)

2

4

X5

Soytone (g/L)

2

4

X6

Yeast extract (g/L)

2

6

X7

Glucose (g/L)

4

8

X8

pH

5

8

X9

Fluconazole (g/L)

1

3

X10

Zn⁺ (g/L)

0.1

0.5

X11

Co⁺ (g/L)

0.1

0.5

X12

Fe²⁺ (g/L)

0.1

0.5

X13

Mg²⁺ (g/L)

0.2

1

X14

Ni⁺ (g/L)

0.1

0.5

X15

Lamifin (g/L)

1

2

X16

Glisofulvin (g/L)

1

2

X17

Methyloleate (g/L)

0.5

1

X18

Incubation day

10

15

X19

Sodium butyrate (g/L)

1

3