What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells?

Table 1 The experimental groups and samples

Model 1: Active Inflammatory state ( inflammatory state induced 18 h before the bacterial stimulation)
K18h	bacteria in co-culture with CaCo2/HT29 (control)
18 h	bacteria in co-culture with CaCo2/HT29 in inflammatory state induced 18 h prior bacteria addition
Model 2: Protection against the inflammatory state (inflammatory state induced 3 h after bacterial stimulation)
K3h:	bacteria in co-culture with CaCo2/HT29 (control)
3 h:	bacteria in co-culture with CaCo2/HT29 in inflammatory state induced 3 h after bacteria addition
Baseline and shared controls
Baseline	bacteria in MRS medium under anaerobic conditions (conditions recommended for Bifidobacterium culture)
RPMI	bacteria in RPMI 1640 medium
RPMI + TNF-α	bacteria in RPMI 1640 medium with the addition of an inflammatory factor
MRS medium	bacteria in MRS medium under aerobic conditions

ISSN: 1471-2180