Fig. 5
From: Oligoribonuclease mediates high adaptability of P. aeruginosa through metabolic conversion
The regulatory effects of orn on c-di-GMP were dynamic (n = 4). (a) Concentration of c-di-GMP under different culture conditions, n = 3, Significance tested with two-sided student’s t test. *** p < 0.001. (b) Changes of c-di-GMP concentration during biofilm formation, 1 d means initiation of biofilm formation. 3 d means microcolony formation stage. 6 d means the biofilm maturation stage. Significance tested with two-sided student’s t test, followed by multiple comparison correction using the Sidak’s test. **** p < 0.0001. (c) The relative expression levels of wspR in P. aeruginosa was determined by qRT-PCR with rpoD as the control housekeeping gene. Significance tested with two-sided student’s t test. **** p < 0.0001. (d, e) Bacterial activity in bacterial fluid or biofilm. ns means no difference. **** p < 0.0001. (f) Biofilm biomass tested by crystal violet assay. Significance tested with two-sided student’s t test, followed by multiple comparison correction using the Sidak’s test. **** p < 0.0001