Fig. 3

orn deletion reduces the production of pyocyanin and rhamnolipids in P. aeruginosa (n = 4). (a) Changes of proteins were associated with the quorum sensing and phenazine biosynthesis of P. aeruginosa whithout orn. Red boxes represent increased proteins, white boxes represent unchanged proteins and blue boxes represent decreased proteins. The shade of color is positively correlated with the protein expression. (b) Rhamnolipids concentration. Significance tested with two-sided student’s t test. ****p < 0.0001. (c) The relative expression levels of lasR, lasI, rhlR and rhlI in P. aeruginosa were determined by qRT-PCR with rpoD as the control housekeeping gene. Significance tested with two-sided student’s t test, followed by multiple comparison correction using the Sidak’s test. *p < 0.05. (d) Production of pyocyanin in continuous culture within three days. Significance tested with two-sided student’s t test, followed by multiple comparison correction using the Sidak’s test. ****p < 0.0001