Fig. 5

The microbiome compositions are similar between the lesion surface and the lesioned body wall. Alpha diversity of the microbiomes from the LS and LBW sample groups are evaluated by A Observed Species, B Chao1, and C ACE. The box plots show the mean and quartile values for each group, which are not different (ANOVA, p > 0.05). D Beta diversity is evaluated at the ASV level using weighted UniFrac and visualized with NMDS. Ellipses around sample groups show 95% confidence intervals assuming a multivariate t-distribution (solid line) and a multivariate normal distribution (dashed line). The microbiomes of the LS compared to the LBW sample groups are not significantly different (PERMANOVA, p > 0.05). The sSW sample is shown for comparison. Sample name abbreviations are defined in Table 1