The rumen-derived Lact. mucosae LLK-XR1 exhibited greater free gossypol degradation capacity during solid-state fermentation of cottonseed meal and probiotic potential

Table 2 Effects of different carbon sources on the growth of Lact. mucosae LLK-XR1 and its degradation rate of free gossypol

Items	Treatments				SEM	P-value
Items	CONL	GFFG	HGFG	TGFG	SEM	P-value
OD600
6 h	/	0.072 ^aA	0.678 ^bA	0.682 ^bA	0.022	< 0.01
12 h	/	0.165 ^aB	1.171 ^bB	1.219 ^bB	0.017	< 0.01
24 h	/	0.451 ^aC	0.890 ^bC	1.334 ^cC	0.010	< 0.01
D_FG (%)
6 h	1.261 ^a	31.387 ^bA	42.836 ^cA	6.581 ^dA	0.769	< 0.01
12 h	1.751 ^a	47.354 ^bB	59.863 ^cB	46.305 ^bB	1.525	< 0.01
24 h	1.443 ^a	47.644 ^bB	59.926 ^cB	69.512 ^dC	0.962	< 0.01

CONL: saline solution & L-MRS medium with 0.01% (w/v) free gossypol; GFFG: culture suspensions + L-MRS medium with glucose-free but 0.01% (w/v) free gossypol; HGFG: culture suspensions+ L-MRS medium with half glucose (1% (w/v) glucose) and 0.01% (w/v) free gossypol; WGFG: culture suspensions + L-MRS medium with 0.01% (w/v) free gossypol;
^{a, b, c} Values in a line within the same class without a common superscript are significantly different (P < 0.05);
^{A, B, C} Values in a column within the same class without a common superscript are significantly different (P < 0.05)

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