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Table 1 Preparation of medium components

From: The rumen-derived Lact. mucosae LLK-XR1 exhibited greater free gossypol degradation capacity during solid-state fermentation of cottonseed meal and probiotic potential

No.

Medium components

1.

S-Screening medium 1

2.

L-LB, S-LB 2

3.

L-MRS, S-MRS 3

4.

TGFG L-MRS4

5.

GFFG L-MRS 5

6.

HGFG L-MRS 6

  1. 1 S-Screening medium: the solid media with 0.5% (w/v) (NH4)2SO4, 0.1% (w/v) KH2PO4, 0.1% (w/v) NaCl, 0.05% (w/v) MgSO4.7H2O, 0.01% (w/v) CaCl2, 0.02% (w/v) yeast extract, 1.5% (w/v) agar and 0.1% (w/v) free gossypol as the sole carbon source;
  2. 2 L-LB: the liquid media prepared from the composition of standard LB broth (China Haibo Biotechnology Co., Ltd., Qingdao, China). S-LB: the solid media with L-LB medium and 1.5% (w/v) agar;
  3. 3 L-MRS: the liquid media prepared from the composition of standard Man-Rogosa-Sharpe (MRS) broth (China Haibo Biotechnology Co., Ltd., Qingdao, China). S-MRS: the solid media with L-MRS medium and 1.5% (w/v) agar;
  4. 4 TGFG L-MRS: the L-MRS medium with 0.01% (w/v) free gossypol;
  5. 5 GFFG L-MRS: the L-MRS medium with glucose-free but 0.01% (w/v) free gossypol;
  6. 6 HGFG L-MRS: the L-MRS medium with half glucose (1% (w/v) glucose) and 0.01% (w/v) free gossypol;
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BMC Microbiology

ISSN: 1471-2180

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