Fig. 5

B. longum SX-1326 improves intestinal inflammation in CRC and relieves diarrhea caused by chemotherapy alone. A H&E staining to observe colonic inflammatory cell infiltration and morphological changes (200 ×); B-D RT-qPCR detected the expression of inflammatory factors TNF-α, IL-6 and IL-1β in the intestines of mice in each group. E WB detection of expression of TLR4-MyD88-p65-p-p65 signaling pathway protein in the gut; F–H WB results for TLR4, MyD88, p65 and p-p65 were quantified using ImageJ; I WB detection of intestinal COX-2 expression; J Quantitative analysis of WB results for COX-2 using ImageJ; K WB detection of expression of barrier proteins Claudin-1 and Occludin in the gut; L-M WB results for Claudin-1 and Occludin were quantified using ImageJ; N COX-2 and MUC-2 expression in colorectal tissues was detected by immunohistochemical staining (200 ×). Experimental group: C: normal control group; M: colorectal cancer model group; B: Bifidobacterium longum SX-1326 group; I: Irinotecan chemotherapy group; U: Bifidobacterium longum SX-1326 plus irinotecan dosing group. Data are expressed as mean ± SD, *p < 0.05, **p < 0.01