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Fig. 4 | BMC Microbiology

Fig. 4

From: Unveiling the fructose metabolism system in Staphylococcus aureus: insights into the regulatory role of FruR and the FruRKT operon in bacterial fitness

Fig. 4

(A) Expression and purification of FruR protein. SDS-PAGE of cell lysates from E. coli BL21 (lane 1), BL21 carrying pET-22b (+)_fruR induced by IPTG (lane 2), and purified FruR protein (lane 3). (B) EMSA of the specific binding of purified FruR protein to the fruRKT promoter region F0 (-105 to 10). (C) DNase I footprinting assay to determine FruR binding site. The FruR protein protected region showed a significantly reduced peak intensities (red) pattern than seen in compared with those of the control (blue). (D) EMSA of the specific binding of purified FruR protein to the shorten fruRKT promoter regions F1 (-85 to -50)

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BMC Microbiology

ISSN: 1471-2180

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