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Fig. 3 | BMC Microbiology

Fig. 3

From: Unveiling the fructose metabolism system in Staphylococcus aureus: insights into the regulatory role of FruR and the FruRKT operon in bacterial fitness

Fig. 3

(A) RT-qPCR analysis of fruK and fruK genes expression levels in S. aureus USA300 WT, Δ03665 (fruR), and Δ03665_com strains cultured in TSB medium. Expression levels were normalized to the WT. (B) Beta-galactosidase-based promoter activity assay. S. aureus USA300 WT and Δ03665 (fruR) strains carrying the pQLV003 derived PfruRKT_lacZ reporter plasmid were grown on TSB X-gal plates at 37 °C for 24 h (up panel). The beta-galactosidase assay of the cell lysates of each strain was quantified, and the strains containing pQLV003 empty vector were used as a control. (****P ≤ 0.0001. Each assay was performed three biological replicates and the data was presented as the mean plus standard deviation)

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BMC Microbiology

ISSN: 1471-2180

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