Fig. 8

Representative TEM images of P. aeruginosa PaO1 incubated with 6xHis-Hill_BB_C10074 and Growth and Buffer controls after 8-hours incubation at 37 °C. TEM showed that 8-hour exposure to 6xHis-Hill_BB_C10074 (500 µg/mL) affected membrane integrity and induced membrane leakage, formation of small spherical cells and lysis of rod-like cells, but some cells appeared healthy and rod-like. There were bacteria in the Growth control population that appeared to be swollen. Some cells in the Buffer control (50mM TrisHCl pH 8.0) were blebbing whilst other cells appeared healthy. Blue arrow = vesicles, orange arrow = leakage of intracellular contents, green arrow = lysing cells, purple arrows = blebbing, red arrow = spherocyte. TEM images shown were selected as representative examples from 3 biological replicates. Scale bars are shown, and letter codes designate the distances of (A) as 20 nm, (B) as 100 nm, (C) as 500 nm, and (D) as 2000 nm