Fig. 7

Representative TEM images of P. aeruginosa PaO1 incubated with 6xHis-Hill_BB_C10074 and Growth and Buffer controls after 2-hours incubation at 37 °C. TEM indicated that 2-hour exposure to 6xHis-Hill_BB_C10074 (500 µg/mL) affected membrane integrity and induced membrane leakage, formation of small spherical cells and lysis of rod-like cells. Whilst bacteria exposed to the Growth control did not appear to have any membrane damage. Bacterial cells exposed to the Buffer control (50mM TrisHCl pH 8.0) demonstrated some changes in membrane integrity. Blue arrow = vesicles, orange arrow = leakage of intracellular contents, green arrow = lysing cells, yellow arrow = cellular debris, purple arrows = blebbing, red arrow = spherocyte. TEM images shown were selected as representative examples from 3 biological replicates. Scale bars are shown, and letter codes designate the distances of (A) as 20 nm, (B) as 100 nm, and (C) as 500 nm