Fig. 4
Optical Density measurements of 3 species of bacteria exposed to 6xHis-Hill_BB_C10074. A S. aureus NCTC 10,788, B E. coli K12, and C P. aeruginosa PaO1 were grown in 200 µL wells containing Hill_BB_C10074 (250 µg/mL) for 8-hours. OD605nm of the cultures were measured periodically and changes in absorbance indicated alterations in growth of the populations. Growth and Buffer (25 mM TrisHCl pH 8.0) controls were included. Data is representative of 3 independent experiments. Error bars represent standard deviations of the mean. D AUCs for each of the growth curves were calculated. Error bars represent 95% CI. Symbols denote significant reduction in AUC between populations. * = 6xHis-Hill_BB_C10074 (250 µg/mL) population reduced compared to the Growth control population. □ = 6xHis-Hill_BB_C10074 (250 µg/mL) population reduced compared to Buffer control population. Populations labelled with the combination of *□ were reduced in size as a direct effect of 6xHis-Hill_BB_C10074 (250 µg/mL)