Table 4 Phenotypic and genotypic characterization of mcr positive E. coli from broiler chicken and farm environment (n = 48)

KBF1

Fecal

165

CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM

≥ 4

A

mcr-4, mcr-6, CTX

ast, iss,irp2

F6(2)

Fecal

605

CIP,AMP,CHL,TET,SXT,STR,NAL,GEN

≥ 4

A

mcr-1

ast,irp2

19D(2)

Evt swab

7506

CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM,GEN

≤ 2

B1

mcr-7

iss

BFd8H3

Food

1771

CIP,AMP,CHL,TET,SXT,STR,NAL,

≤ 2

A

mcr-8

ast,iss,irp2

ZC5B

cloacal

1771

CIP,AMP,CHL,TET,SXT,STR,NAL,

≤ 2

C

mcr-8

ast

B9H2

cloacal

155

CIP,AMP,CHL,TET,SXT,STR,

≤ 2

E

mcr-1

ast, iss,irp2,iucD

12B

cloacal

48

AMC,CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,

≤ 2

Clade I or II

mcr-1

ast, iss,irp2,iucD

3BA

cloacal

1703

CIP,AMP,CHL,TET,SXT,STR,NAL

≤ 2

A

mcr-1

ast,pap C

ZC4BN

cloacal

206

CIP,AMP,CHL,TET,STR,

≤ 2

C

mcr-8, SHV

ast

C39H4

cloacal

1771

CIP,AMP,CHL,TET,SXT,STR,NAL

≤ 2

A

mcr-8, CTX

ast,papC

YC1H2

cloacal

354

CIP,AMP,CHL,TET,SXT,STR,NAL,GEN

≥ 4

F

mcr-6

ast

Dr7H2

Water

1771

CIP,AMP,CHL,TET,SXT,STR,NAL,

≥ 4

A

mcr-8

irp2

E15(2)

Evt swab

206

CAZ,AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM, GEN

≥ 4

A

mcr-1

ast,tsh,iss

KB4A

cloacal

162

CIP,AMP,CHL,TET,SXT,STR,NAL,GEN

≤ 2

B1

mcr-9, CTX

ast,iss, iuc D

BFd5H4

Food

159

CIP,AMP,CHL,TET,SXT,STR,NAL,CXM,

≥ 4

C

mcr-1,mcr-5

ast,iss, iuc D

B8H4

cloacal

11,630

AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM

≤ 2

B1

mcr-6

ast, iuc D

B7H4

cloacal

38

CIP,AMP,CHL,TET,SXT,STR,NAL,GEN

≥ 4

D

mcr-7, mcr-1

ast,iss,papC,iucD

E43A

Evt swab

398

CIP,AMP,CHL,TET,SXT,STR,NAL,

≥ 4

F

mcr-6, CTX

ast,iss

Drw3

Water

206

CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,

≥ 4

A

mcr-1, mcr-8, SHV

ast

Drw7

Water

1285

CIP, AMP,CHL,TET,SXT,STR,NAL,CXM,GEN

≥ 4

B1

mcr-1, SHV

_

WF5

Swage

1140

CAZ,AMC,CIP,AMP,CHL,TET,SXT,STR,NAL,CXM

≥ 4

D

mcr-7

iss,irp2

L25B

Litter

106

CAZ,AMC,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM

≥ 4

D

mcr-1, SHV,CTX

irp2

Food_G

Food

43

AMP,CHL,TET,SXT,STR,NAL

≥ 4

C

mcr-1

_

7a

cloacal

_

CIP,IPM,AMP,CHL,TET,SXT,STR,NAL

≥ 4

D

mcr-6

_

B2H1

cloacal

_

CIP,AMP,CHL,TET,SXT,STR,NAL,

≥ 4

B2

mcr-6

_

BFood7H1

Food

CIP,AMP,CHL,TET,SXT,STR,NAL,CXM

≤ 2

A

mcr-7

_

Drw1

Water

_

CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,

≥ 4

D

mcr-8

_

Drw4

Water

_

CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,

≥ 4

B1

mcr-8

_

E1

Evt swab

_

CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM,GEN

≥ 4

A

mcr-1

_

E21

Evt swab

CIP,AMP,CHL,TET,SXT,STR,

≤ 2

Unknown

mcr-7

E22

Evt swab

CIP, AMP,CHL,TET,SXT,STR,NAL,

≤ 2

A

mcr-1

_

E28

Evt swab

CIP,AMP,CHL,TET,SXT,STR,NAL,

≤ 2

E

mcr-1

EVT11

Evt swab

AMC,CIP,AMP,CHL,TET,SXT,STR,NAL,

≤ 2

A

mcr-7

EVT16

Evt swab

_

CIP,AMP,CHL,TET,SXT,STR,NAL,GEN

≥ 4

A

mcr-1

_

EVT21

Evt swab

_

CIP,AMP,CHL,TET,SXT,STR,ATM,NAL,

≥ 4

A

mcr-1

_

F1

Fecal

_

CIP,AMP,CHL,TET,SXT,STR,NAL,

≥ 4

A

mcr-7

_

L16

Litter

_

CIP,AMP,CHL,TET,SXT,STR,NAL,

≥ 4

A

mcr-1

_

L17

Litter

_

CIP,AMP,CHL,TET,SXT,STR,ATM,NAL

≥ 4

C

mcr-8

_

YC23H2

cloacal

CIP,AMP,CHL,TET,SXT,STR,

≤ 2

A

mcr-1

ast

ZC13H1

cloacal

CIP, AMP,CHL,TET,SXT,STR,NAL,GEN

≤ 2

A

mcr-1

ZC22H1

cloacal

_

AMP, CHL,TET,STR

≥ 4

B1

mcr-1

_

EVT13

Evt swab

AMC, CIP,AMP,CHL,TET,SXT,STR,NAL,CXM

≤ 2

A

mcr-1

Fecal

Fecal

CIP, AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,CXM

≤ 2

Clade I or II

mcr-1

FoodB

Food

_

CAZ, MEM,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM

≥ 4

A

mcr-1

_

FoodD

Food

AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM,GEN

≤ 2

A

mcr-1

11a

cloacal

AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM

≤ 2

A

mcr-6

E2

Evt swab

_

CIP, AMP, CHL,TET,SXT,STR,NAL,GEN

≥ 4

A

mcr-8

_

12a

cloacal

CAZ,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM,GEN

≤ 2

A

mcr-6