Fig. 6

Modulation of immunoregulatory and barrier-related genes expression in Caco-2 cells. The relative gene expression of genes related to immunomodulation and intestinal barrier (Il1b (A and B), Il8 (C and D), Mcp1 (E and F), Nfkb1a (G and H), Ptgs2 (I and J), Tnf (K and L), Ocln (M and N), and Muc2 (O and P)) on CEC15- and EcN-treated cells, respectively, were evaluated with the Gapdh, B2m, and Hprt1 genes as reference (2^{- ΔΔct}). Statistical analyses were performed by One-way ANOVA with Tukey’s post-test on GraphPad Prism 7.0. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001. NC: negative control; CEC15-SN: CEC15 supernatant; CEC15-M100: CEC15 treatment at MOI 100; CEC15-M10: CEC15 treatment at MOI 10; EcN-SN: EcN supernatant; EcN-M100: EcN treatment at MOI 100; EcN-M10: EcN15 treatment at MOI 10