Whole genome sequencing and pan-genome analysis of Staphylococcus/Mammaliicoccus spp. isolated from diabetic foot ulcers and contralateral healthy skin of Algerian patients

Table 4 Resistance gene profiles in S. aureus isolates recovered from DFUs and Healthy skin (n = 32)

	ST1-t127 (n = 2) n(%)	ST241-t037 (n = 5) n(%)	ST291-t937 (n = 1) n(%)	ST672-t3841 (n = 3) n(%)	ST80-t044/t1247/t639 (n = 16) n(%)	ST97-t9432 (n = 1) n(%)	STNF-t037 (n = 4) n(%)	Total (n = 32) n(%)	*p-value
β-lactams
mecA	0(0)	5(100)	0(0)	3(100)	16(100)	0(0)	3(75)	27(84.4)	< 0.001
blaZ	0(0)	5(100)	1(100)	3(100)	2(12.5)	1(100)	4(100)	16(50)	< 0.001
blaI	0(0)	0(0)	0(0)	1(33.3)	0(0)	1(100)	0(0)	2(6.3)	0.002
blaR1	0(0)	0(0)	0(0)	1(33.3)	0(0)	1(100)	0(0)	2(6.3)	0.002
Aminoglycoside
ant(6)-Ia	0(0)	0(0)	0(0)	0(0)	16(100)	1(100)	0(0)	17(53.1)	< 0.001
ant(9)-Ia	0(0)	5(100)	0(0)	0(0)	0(0)	0(0)	0(0)	5(15.6)	< 0.001
aac(6’)- aph(2’’)	0(0)	5(100)	0(0)	0(0)	0(0)	0(0)	3(75)	8(25)	< 0.001
aph(3’)-III	0(0)	5(100)	0(0)	2(66.7)	13(81.3)	0(0)	0(0)	20(62.5)	0.005
aph(3’)-IIIa	0(0)	0(0)	0(0)	1(33.3)	3(18.8)	1(100)	0(0)	5(15.6)	0.207
sat4	0(0)	0(0)	0(0)	1(33.3)	3(18.8)	1(100)	0(0)	5(15.6)	0.207
Macrolides
mph(C)	0(0)	0(0)	0(0)	3(100)	0(0)	0(0)	0(0)	3(9.4)	< 0.001
msr(A)	0(0)	0(0)	0(0)	3(100)	0(0)	0(0)	0(0)	3(9.4)	< 0.001
ermA	0(0)	5(100)	0(0)	0(0)	0(0)	0(0)	0(0)	5(15.6)	< 0.001
ermC	0(0)	0(0)	0(0)	0(0)	1(6.3)	1(100)	0(0)	2(6.3)	0.014
Tetracycline
tet(K)	0(0)	0(0)	0(0)	0(0)	2(12.5)	0(0)	0(0)	2(6.3)	0.907
tet(M)	0(0)	5(100)	0(0)	0(0)	0(0)	0(0)	4(100)	9(28.1)	< 0.001
tet(38)	1(50)	0(0)	0(0)	1(33.3)	3(18.8)	1(100)	0(0)	6(18.8)	0.214
Fusidic acid
fusB	0(0)	0(0)	0(0)	0(0)	2(12.5)	0(0)	0(0)	2(6.3)	0.907
fusC	2(100)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	2(6.3)	< 0.001
Trimethoprim-sulfamethoxazole
dfrG	0(0)	5(100)	0(0)	0(0)	0(0)	0(0)	0(0)	5(15.6)	< 0.001
Fosfomycin
fosB	0(0)	0(0)	0(0)	1(33.3)	0(0)	0(0)	0(0)	1(3.1)	0.126

*Chi-squared test was used to calculate p-values for describing the association between genes and STs, p-values < 0.05 were considered statistically significant
Values are numbers and percentages in brackets

ISSN: 1471-2180