Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Table 1 Primer sequences for the target genes

Gene ID/ Name	Primers	Amplicon size (bp)
PA0524 (norB)	FP: 5’-CTACAACCCGGAAAACCTCA-3’	228
PA0524 (norB)	RP: 5’-ACGCCGATCCAGAAGTAGTG-3’	228
PA0521 (Cytochrome c subunit)	FP: 5’-CGAGCACGACACTTTCTTCA-3’	172
PA0521 (Cytochrome c subunit)	RP: 5’-GACCATGTGCCAGTAGAGCA-3’	172
PA3877 (narK1)	FP: 5’ -AAGACCGCATCCTTCATCAC-3’	238
PA3877 (narK1)	RP: 5’ -ACACCAGGAAGGTGAACAGC-3’	238
PA3617 (recA) (selected as a negative control, not expected to express differently)	FP: 5’-CGTCAAGGTGGTGAAGAACA − 3’	231
	RP: 5’-TCCAGTACCGAACCGATTTC − 3’	231

ISSN: 1471-2180