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Fig. 1 | BMC Microbiology

Fig. 1

From: Antimicrobial peptidase lysostaphin at subinhibitory concentrations modulates staphylococcal adherence, biofilm formation, and toxin production

Fig. 1

(A) Schematic representation of the full length pre-proAPLss (∼53-kDa) displaying the leader sequence (LS), proregion (PRO), catalytic domain (CAT), linker (LK), and cell wall-targeting domain (CWT). The ∼28-kDa APLss was incorporated with a 6× His tag at the C terminus. (B) SDS-PAGE (Coomassie brilliant blue-stained 12% gel) analysis of crude extracts from E. coli cells expressing APLss (lane 2), uninduced E. coli cells as a negative control (lane 1), the soluble fraction of cell lysate after centrifugation (lane 3), and M represents protein marker. (C) The corresponding Western blot probed with anti-His tag antibody followed by ALP-conjugated secondary antibody. M, protein marker; Lane 1, E. coli lysate fraction containing APLss; and Lane 2, E. coli soluble fraction containing APLss. (D) SDS-PAGE (12% gel) of the purified APLss protein via IMAC (lane 1 and 2) and M represents protein marker

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BMC Microbiology

ISSN: 1471-2180

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