Fig. 6

Addition of ERCC spike in controls prior to or after rRNA depletion to measure the accuracy of rRNA depletion and potential library prep bias caused by the DP1 and HMv2 probes. A ERCC controls were spiked into the library prep either immediately before or after rRNA depletion. B Spearman correlation coefficients between different groups tested. C-F Representative pairwise correlations between ERCC spike-in abundance for sample pairs with the following characteristics: C) pre-depletion, different donors, D) post-depletion, different donors, E) pre- vs. post-depletion, same donor, F) pre- vs. post-depletion, different donors