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Fig. 3 | BMC Microbiology

Fig. 3

From: Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes

Fig. 3

The Pan-Human Microbiome probe pool, HMv1, effectively depletes rRNA in stool samples from healthy adult donors to generate metatranscriptomic profiles. A Percentage of reads mapping to eukaryotic and prokaryotic rRNAs vs. coding sequences in samples depleted with Ribo-Zero Plus standard probes (DP1) vs combination of DP1 and HMv1 probes (stool RNA samples from 10 healthy adult donors). Boxed bar plot on top represents remaining bacterial rRNA (LSU & SSU) for either DP1-depleted samples (black bars) or HMv1-depleted samples (grey bars) with a dashed line at the 50% mark. B Taxonomic heatmap showing the 20 most abundant taxa for each sample based on metatranscriptomic analysis of adult stool samples. C Number of reads, taxonomic and functional features detected in undepleted, DP1-depleted, or HMv1-depleted stool samples

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BMC Microbiology

ISSN: 1471-2180

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