Fig. 4

Mycothiol (MSH) is needed for optimal actinorhodin (ACT) synthesis. a Spores were inoculated onto yeast extract-malt extract-glutamine agar medium with a toothpick and cultured for 96 h at 30 °C. b M145 (blue bar), ∆mshC (red bar), and ∆mshC::mshC (yellow bar) strains were cultured under the same conditions as those in (a). Total RNA was extracted from each strain to measure the relative expression level of actII-ORF4 using reverse transcription-quantitative polymerase chain reaction. Data are presented as mean and standard deviation values from three independent experiments. ***p < 0.001 (Student’s t-test)