Fig. 3

Defect of mycothiol (MSH) synthesis decreases low-molecular-weight (LMW) S-nitrosothiol (SNO) levels. M145 (blue bars), ∆mshC (red bars), and ∆nar (yellow bars) strains were cultured for the indicated durations. After deproteinisation, SNO was converted to NO₂⁻ using Hg.²⁺ as a catalyst. SNO levels were measured using the Saville–Griess assay. Concentrations below the detection limit (500 nM) were recorded as the detection limit concentration. Data are presented as the mean and standard deviation values from three or four independent experiments. ***p < 0.001; *p < 0.05; NS, not significant (Tukey–Kramer test)