Fig. 2
Mycothiol (MSH) gene deletion decreases intracellular nitric oxide (NO) levels. a Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis of the relative expression levels of hmpA in the total RNA extracted from strains M145 (blue bars), ∆mshC (red bars), and ∆mshC::mshC (yellow bars) cultured for the indicated durations. Data are presented as the mean and standard deviation values from six independent experiments. **p < 0.01; *p < 0.05; NS, not significant (Student’s t-test). b Relative mRNA expression levels of devR in the total RNA extracted from strains M145 (blue bars), ∆mshC (red bars), and ∆mshC::mshC (yellow bars) cultured for the indicated durations. Data are presented as the mean and standard deviation values from three independent experiments. **p < 0.01, *p < 0.05 (Student’s t-test). c Relative mRNA expression levels of hmpA in the total RNA extracted from M145 and ∆mshC strains. After the strains were cultured for 72 h, they were treated with 1 ml water (control; blue bars), 100 μM NO2.− for 1 h (red bars), or 1 μM NOC5 solution (yellow bars); incubated for 30 min; and subjected to RT-qPCR analysis. Data are presented as mean and standard deviation values from three independent experiments. *p < 0.05; NS, not significant (Dunnett’s test)