Fig. 1

Effect of mycothiol (MSH) deficiency on colony growth. a Construction of the mshC disruptant strain. The plasmid pGM160, including the 2,000 nucleotides upstream and downstream of mshC flanking an aac(3)IV gene, was transformed into Streptomyces coelicolor A3(2) M145. The double-crossover event is shown schematically. b The colony area of strains M145 (blue bars), ∆mshC (red bars), and ∆mshC::mshC (yellow bars) cultured for the indicated durations. Data are presented as mean and standard deviation (n = 9). ***p < 0.001; NS, not significant (Student’s t-test). c The wet weight of M145 (blue bars), ∆mshC (red bars), and ∆mshC::mshC (yellow bars) strains cultured for the indicated durations. Data are presented as mean and standard deviation values from three independent experiments. ***p < 0.001; *p < 0.05. NS, not significant (Student’s t-test). d The colony area of ∆nar (blue bars) and ∆nar/mshC (red bars) strains cultured for the indicated durations. Data are presented as mean and standard deviation (n = 9) from three independent experiments. e The wet weight of ∆nar (blue bars) and ∆nar/mshC (red bars) strains cultured for the indicated durations. Data are presented as the mean and standard deviation values from three independent experiments. ***p < 0.001; **p < 0.01; NS, not significant (Student’s t-test)