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Table 3 HA production by H15 strain under different C and N sources

From: Hyaluronic acid production by Klebsiella pneumoniae strain H15 (OP354286) under different fermentation conditions

C and N sources

Concentrations of HA (mg L−1)

HPLC method

Turbidity method

Carbazole method

Glucose

751 ± 29.0a

785 ± 27.0a

796 ± 64.0a

Sucrose

889 ± 49.0a

919 ± 63.0a

941 ± 93.0a

Lactose

544 ± 21.0c

627 ± 30.0c

654 ± 27.0c

Maltose

464 ± 41.0c

531 ± 52.0c

554 ± 44.0c

Fructose

772 ± 23.0a

843 ± 44.0a

854 ± 52.0a

Peptone

555 ± 40.0b

661 ± 29.0b

683 ± 37.0b

Yeast extract

751 ± 34.0a

861 ± 37.0a

878 ± 41.0a

Yeast extract + peptone

892 ± 36.0a

924 ± 44.0a

956 ± 45.0a

Ammonium sulfate

000 ± 000

000 ± 000

000 ± 000

Sodium nitrate

000 ± 000

000 ± 000

000 ± 000

  1. Calculated mean is for triplicate measurements from two independent experiments ± SD; means with different superscripts in the same column either for C or N sources are considered statistically different (LSD test, P ≤ 0.05). In the case of testing C sources, the cultivation medium contained combination of yeast extract and peptone as N source. In the case of testing N sources, the cultivation medium contained sucrose as C source
  2. Different letters on the bars (a, b, c) indicate significant differences between the data points. Bars with the same letter are not significantly different, while bars with different letters are
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BMC Microbiology

ISSN: 1471-2180

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