Screening of high-efficiency nitrogen-fixing bacteria from the traditional Chinese medicine plant Astragalus mongolicus and its effect on plant growth promotion and bacterial communities in the rhizosphere

Table 1 PCR reaction system and conditions of 16S rRNA

Reaction system	2 × mix 20 µL, Primer27F 1.5 µL, Primer1492R 1.5 µL, ddH₂O 15 µL, Template gDNA 2 µL
Reaction process	Predenaturation	Transsexual	Refolding	Extend
Temperature Reflex	95 ℃	95 ℃	52 ℃	72 ℃
Reaction time	3 min	30 s	30 s	7 min

ISSN: 1471-2180